Inter-laboratory Testing of GMO Detection by Combinatory SYBR®Green PCR Screening (CoSYPS)

Barbau-Piednoir, Elodie; Stragier, Pieter; Roosens, Nancy; Mazzara, Marco; Cristian, Savini; Eede, Guy Van den; Bulcke, Marc Van den

doi:10.1007/s12161-014-9837-3

Cited by 14 publications

(11 citation statements)

References 23 publications

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“…In the WIV-ISP GMOlab, currently seventeen inhouse validated screening markers are available for routine detection of GMO in food and feed samples [42]. Eleven of these methods have been fully validated in an EU inter-laboratory test [49] and will be added to the EU Compendium (http://gmo-crl.jrc.ec.europa.eu/gmomethods/). To supplement this list, two new trait-specific SYBR ® Green screening methods have been developed and in-house validated.…”

Section: Resultsmentioning

confidence: 99%

New qualitative trait-specific SYBR®Green qPCR methods to expand the panel of GMO screening methods used in the CoSYPS

Broeders

Fraiture

Vandermassen

et al. 2015

Eur Food Res Technol

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specificity and repeatability were assessed as well as the robustness of the methods. Additionally, the reproducibility was evaluated by transferring the methods to a second laboratory. Both methods allow a specific, sensitive and repeatable detection of the respective targets in food and feed samples and can be easily applied in a routine laboratory. Moreover, they can be used together with previously validated SYBR ® Green qPCR methods to expand the panel of screening methods. This allows an extended coverage of the GM events authorised in the EU and adds discriminative power to the screening phase.

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Section: Resultsmentioning

confidence: 99%

New qualitative trait-specific SYBR®Green qPCR methods to expand the panel of GMO screening methods used in the CoSYPS

Broeders

Fraiture

Vandermassen

et al. 2015

Eur Food Res Technol

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“…Using the JRC GMO-Amplicons, it is possible to identify GM-related sequences that are positive for the elements detected in the analysed samples but negative for the event-specific methods included into the GMOMETHODS database. A case-study example is shown in Figure 5 , where a maize sample was found positive for two construct specific methods, QL-CON-00-011 ( 30 ) and QT-CON-00-005 ( 29 ) and to two element detection methods, QT-ELE-00-001 ( 31 ) and QL-ELE-00-021 ( 32 ). Through the JRC GMO-Amplicons it was possible to identify a new patent sequence record ( 33 ) and to download it for further characterization.…”

Section: Resultsmentioning

confidence: 99%

JRC GMO-Amplicons: a collection of nucleic acid sequences related to genetically modified organisms

et al. 2015

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The DNA target sequence is the key element in designing detection methods for genetically modified organisms (GMOs). Unfortunately this information is frequently lacking, especially for unauthorized GMOs. In addition, patent sequences are generally poorly annotated, buried in complex and extensive documentation and hard to link to the corresponding GM event. Here, we present the JRC GMO-Amplicons, a database of amplicons collected by screening public nucleotide sequence databanks by in silico determination of PCR amplification with reference methods for GMO analysis. The European Union Reference Laboratory for Genetically Modified Food and Feed (EU-RL GMFF) provides these methods in the GMOMETHODS database to support enforcement of EU legislation and GM food/feed control. The JRC GMO-Amplicons database is composed of more than 240 000 amplicons, which can be easily accessed and screened through a web interface. To our knowledge, this is the first attempt at pooling and collecting publicly available sequences related to GMOs in food and feed. The JRC GMO-Amplicons supports control laboratories in the design and assessment of GMO methods, providing inter-alia in silico prediction of primers specificity and GM targets coverage. The new tool can assist the laboratories in the analysis of complex issues, such as the detection and identification of unauthorized GMOs. Notably, the JRC GMO-Amplicons database allows the retrieval and characterization of GMO-related sequences included in patents documentation. Finally, it can help annotating poorly described GM sequences and identifying new relevant GMO-related sequences in public databases. The JRC GMO-Amplicons is freely accessible through a web-based portal that is hosted on the EU-RL GMFF website.Database URL: http://gmo-crl.jrc.ec.europa.eu/jrcgmoamplicons/

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“…Numerous methods have been described to detect GMO-derived material in food, feed and seeds (Fernandez, S., et al, 2005;Žel, J. et al, 2008;Querci, M. et al, Barbau-Piednoir E et al, 2010;Cottenet, G. et al, 2013;Wu, Y., et al 2014;Huber, I. et al, 2013;Barbau-Piednoir E. et al, 2014;Bhoge, R. K. et al, 2016). Most studies in the available literature focuses on the design and development of these methods, but only a few study the GMO content in food found in the market (Cardarelli et.al, 2005;Viljoen, C. D., 2006;Prins, T. W.,et al, 2016;Elsanhoty et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Gmo labeling in food products in montevideo, uruguay

Arleo

Benavente²,

Cardozo³

et al. 2020

Preprint

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Montevideo establishes the mandatory labeling of foods containing genetically modified material through the Departmental Decree No. 36.554, positioning Uruguay within the 65 countries that have incorporated this type of regulation. The Food Regulation Service, in its role of sanitary police, and through its Laboratory of Bromatology, in agreement with the Food Molecular Traceability Laboratory (Faculty of Sciences, University of the Republic), carried out the analysis of 206 products made with ingredients derived from corn and/or soybean, during the 2015-2017 period, within the framework of compliance with the aforementioned Decree. The strategy used consisted of the application of molecular techniques (Real Time PCR), for the detection of common sequences present in the transgenic events of soybean and corn, and the subsequent quantification of the content of GM material, in relation to an established labeling threshold of 1%. As a result of this study, it was found that 36.9% of the analyzed foods presented sequences derived from genetically modified plant organisms (GMOs); and in 95% of the cases, its content exceeded the threshold established for its labeling. This study, constitutes the first approach to the knowledge of different transgenic elements distribution in food commercialized in MontevideoThese results provide valuable information to both the consumer, for decision making about the food to be consumed, and also for the official control organizations, which must enforce the regulations. This type of work has demonstrated, once again, the importance of the interrelation between academy and state agencies, in the generation of knowledge and in the implementation of new analytical methodologies, as well as in the training of qualified human resources and in the compliance with current regulations.

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Inter-laboratory Testing of GMO Detection by Combinatory SYBR®Green PCR Screening (CoSYPS)

Cited by 14 publications

References 23 publications

New qualitative trait-specific SYBR®Green qPCR methods to expand the panel of GMO screening methods used in the CoSYPS

New qualitative trait-specific SYBR®Green qPCR methods to expand the panel of GMO screening methods used in the CoSYPS

JRC GMO-Amplicons: a collection of nucleic acid sequences related to genetically modified organisms

Gmo labeling in food products in montevideo, uruguay

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