Inter-laboratory Validation Study of a Singlet Oxygen Absorption Capacity Assay Method for Determining the Antioxidant Capacities of Antioxidant Solutions and Food Extracts

Wakagi, Manabu; Watanabe, Jun; Takahashi, Sakiko; Yasui, Akemi; Takano-Ishikawa, Yuko

doi:10.3136/fstr.23.481

Cited by 2 publications

(3 citation statements)

References 14 publications

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“…Furthermore, a modified method using a microplate reader has been established, enabling SOAC to be applied as a high throughput assay (Takahashi et al, 2016). This modified SOAC method has been validated in an inter-laboratory test (Wakagi et al, 2017). Previous studies have reported ORAC values of various foods as their antioxidant capacity; however, the values provide only the radical scavenging activity within the total antioxidant activity.…”

Section: Introductionmentioning

confidence: 99%

Daily Radical Scavenging and Singlet Oxygen Quenching Capacity Intake from Fruits and Vegetables in Japan

Takahashi¹,

Tsutsumi²,

Aizawa³

et al. 2018

FSTR

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Radical scavenging and singlet oxygen quenching activities of 32 kinds of fruits and vegetables typically consumed in Japan were evaluated using both the oxygen radical absorbance capacity (ORAC) and singlet oxygen absorption capacity (SOAC) methods. Total-ORAC and SOAC values correlated with total polyphenol (r = 0.92) and carotenoid (r = 0.85) contents. From the data of daily fruit and vegetable intake (110.0 and 286.4 g, respectively) in the National Health and Nutrition Survey in Japan, daily ORAC and SOAC intakes were estimated to be 3177.7 μmol trolox equivalents/day and 1610.3 μmol α-tocopherol equivalents/ day, respectively. The major contributors to ORAC intake were apples, Satsuma mandarins, edible burdock, and onions, accounting for 44.3 % of the total; whereas Japanese squash, carrots, tomatoes, and spinach accounted for 73.2 % of total SOAC intake. Total antioxidant capacity assessment of foods may facilitate the identification of relationships between antioxidant intake and disease risk reduction.

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Section: Introductionmentioning

confidence: 99%

Daily Radical Scavenging and Singlet Oxygen Quenching Capacity Intake from Fruits and Vegetables in Japan

Takahashi¹,

Tsutsumi²,

Aizawa³

et al. 2018

FSTR

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“…The precision of the SOAC assay method was evaluated in an inter-laboratory validation study (41). SOAC values were measured for 3 AO solutions (i.e.…”

Section: Validation Studymentioning

confidence: 99%

“…The SOAC values measured using a microplate reader were in good agreement with the corresponding those measured using an UV-vis spectrophotometer. The SOAC assay method was validated by inter-laboratory validation study due to 14 laboratories (41).…”

Section: Carotenoidmentioning

confidence: 99%

Antioxidant Activity of Foods: Development of Singlet Oxygen Absorption Capacity (SOAC) Assay Method

Mukai

2019

J Nutr Sci Vitaminol

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Recently a new assay method that can quantify the singlet oxygen absorption capacity (SOAC) of antioxidants (AOs) and food extracts was proposed. Singlet oxygen (1 O2) quenching rates (kQ) and the relative SOAC values were measured for many carotenoids and phenolic AOs in ethanol/chloroform/D2O (50 : 50 : 1, v/v/v) solution at 35˚C using UVvis spectrophotometry. It has been clarified that the SOAC method is useful to evaluate the 1 O2-quenching activity of lipophilic and hydrophilic AOs having 5 orders of magnitude different rate constants (kQ). Measurements of the kQ and SOAC values were also performed for 39 kinds of food extracts. The results indicate that the SOAC method is useful to evaluate the 1 O2-quenching activity of food extracts having two orders of magnitude different kQ values. Further, the kQ values for the reaction of 1 O2 with 8 carotenoids and 8 vitamin E homologues were measured in an aqueous Triton X-100 (5.0 wt %) micellar solution (pH 7.4). Results obtained demonstrate that the kQ values of AOs in homogeneous and heterogeneous solutions vary notably depending on (i) polarity (dielectric constant (e)) of the reaction field between AOs and 1 O2, (ii) local concentration of AOs, and (iii) mobility of AOs in solution. Measurements of kQ and SOAC values in a micellar solution may be useful for evaluating the 1 O2-quenching activity of AOs in biological systems. Furthermore, measurements of the SOAC values were performed for 32 kinds of food extracts using a microplate reader. The SOAC assay method was validated by inter-laboratory validation study due to 14 laboratories.

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Inter-laboratory Validation Study of a Singlet Oxygen Absorption Capacity Assay Method for Determining the Antioxidant Capacities of Antioxidant Solutions and Food Extracts

Cited by 2 publications

References 14 publications

Daily Radical Scavenging and Singlet Oxygen Quenching Capacity Intake from Fruits and Vegetables in Japan

Daily Radical Scavenging and Singlet Oxygen Quenching Capacity Intake from Fruits and Vegetables in Japan

Antioxidant Activity of Foods: Development of Singlet Oxygen Absorption Capacity (SOAC) Assay Method

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