Inter‐organ signaling in plants: regulation of ATP sulfurylase and sulfate transporter genes expression in roots mediated by phloem‐translocated compound

Lappartient, Anne G.; Vidmar, J. John; Leustek, Thomas; Glass, Anthony D. M.; Touraine, Bruno

doi:10.1046/j.1365-313x.1999.00416.x

Cited by 269 publications

(188 citation statements)

References 33 publications

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“…34 It has been reported that withdrawal of S from the medium leads to decreased levels of sulfate and GSH in Arabidopsis and canola plants tissues resulting in the induction of sulfate transporter systems and ATPS activity. 35,36 The inhibition in the activity of ATPS1 and APR results in the inhibition of GSH synthesis. 37,38,39 Supplementation of 0.5 mM SA results in the substantial increase in ATP sulfurylase, serine acetyl transferase activity and Cys content under salt stress and helps in reversing the effects of NaCl ¡ induced ROS on photosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Exogenous salicylic acid improves photosynthesis and growth through increase in ascorbate-glutathione metabolism and S assimilation in mustard under salt stress

Nazar

Umar

Khan

2015

Plant Signaling & Behavior

202

114

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Ascorbate (AsA)-glutathione (GSH) cycle metabolism has been regarded as the most important defense mechanism for the resistance of plants under stress. In this study the influence of salicylic acid (SA) was studied on ascorbateglutathione pathway, S-assimilation, photosynthesis and growth of mustard (Brassica juncea L.) plants subjected to 100 mM NaCl. Treatment of SA (0.5 mM) alleviated the negative effects of salt stress and improved photosynthesis and growth through increase in enzymes of ascorbate-glutathione pathway which suggest that SA may participate in the redox balance under salt stress. The increase in leaf sulfur content through higher activity of ATP sulfurylase (ATPS) and serine acetyl transferase (SAT) by SA application was associated with the increased accumulation of glutathione (GSH) and lower levels of oxidative stress. These effects of SA were substantiated by the findings that application of SAanalog, 2,6, dichloro-isonicotinic acid (INA) and 1 mM GSH treatment produced similar results on rubisco, photosynthesis and growth of plants establishing that SA application alleviates the salt-induced decrease in photosynthesis mainly through inducing the enzyme activity of ascorbate-glutathione pathway and increased GSH production. Thus, SA/GSH could be a promising tool for alleviation of salt stress in mustard plants.

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Section: Discussionmentioning

confidence: 99%

Exogenous salicylic acid improves photosynthesis and growth through increase in ascorbate-glutathione metabolism and S assimilation in mustard under salt stress

Nazar

Umar

Khan

2015

Plant Signaling & Behavior

202

114

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“…1B). The phylogenic relationships of plant sulfate transporters indicated that Sultr1;3 falls into the group of highaffinity sulfate transporters in the vascular plants (Smith et al, 1995(Smith et al, , 1997Vidmar et al, 1999;Takahashi et al, 2000;Shibagaki et al, 2002;Yoshimoto et al, 2002;Fig. 2).…”

Section: Identification Of a Novel High-affinity Sulfate Transportermentioning

confidence: 99%

“…Glutathione and S-methyl-Met are the major sulfur compounds in the phloem sap (Bourgis et al, 1999). Furthermore, glutathione translocated in the phloem is suggested to mediate transmission of the interorgan signal of sulfur status in vascular plants (Lappartient et al, 1999). Studies with poplar more recently suggested a correlation between the demand of sulfur in shoots and the sulfate to glutathione ratio in the phloem sap (Herschbach et al, 2000).…”

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confidence: 99%

“…In the past few years, numbers of genes encoding high-affinity sulfate transporters in vascular plants have been isolated and characterized (Smith et al, 1995(Smith et al, , 1997Vidmar et al, 1999;Takahashi et al, 2000;Shibagaki et al, 2002;Yoshimoto et al, 2002). These high-affinity sulfate transporters are predominantly expressed in roots of sulfur-starved plants and are suggested to serve for the initial uptake of sulfate from the soil.…”

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confidence: 99%

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Phloem-Localizing Sulfate Transporter, Sultr1;3, Mediates Re-Distribution of Sulfur from Source to Sink Organs in Arabidopsis

et al. 2003

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For the effective recycling of nutrients, vascular plants transport pooled inorganic ions and metabolites through the sieve tube. A novel sulfate transporter gene, Sultr1;3, was identified as an essential member contributing to this process for redistribution of sulfur source in Arabidopsis. Sultr1;3 belonged to the family of high-affinity sulfate transporters, and was able to complement the yeast sulfate transporter mutant. The fusion protein of Sultr1;3 and green fluorescent protein was expressed by the Sultr1;3 promoter in transgenic plants, which revealed phloem-specific expression of Sultr1;3 in Arabidopsis. Sultr1;3-green fluorescent protein was found in the sieve element-companion cell complexes of the phloem in cotyledons and roots. Limitation of external sulfate caused accumulation of Sultr1;3 mRNA both in leaves and roots. Movement of 35 S-labeled sulfate from cotyledons to the sink organs was restricted in the T-DNA insertion mutant of Sultr1;3. These results provide evidence that Sultr1;3 transporter plays an important role in loading of sulfate to the sieve tube, initiating the source-to-sink translocation of sulfur nutrient in Arabidopsis.

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“…Exogenous application of GSH downregulated expression of a sulfur transporter gene Sultr2;2, and a gene encoding ATP sulfurylase (Lappartient et al 1999). Exogenous application of OAS resulted in upregulation of expression of genes encoding adenosine 5Ј-phosphosulfate reductase, chloroplastic and cytosolic cysteine synthase and serine acetyltransferase (Koprivova et al 2000).…”

mentioning

confidence: 99%

Independent roles of glutathione and O-acetyl-L-serine in regulation of sulfur-responsive gene expression in Arabidopsis thaliana

Sogawa

Ohkama‐Ohtsu

Hayashi

et al. 2005

Plant Biotechnology

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We investigated the effects of a four-day treatment with 0.3 mM glutathione (GSH) and 10 mM O-acetyl-Lserine (OAS), negative and positive regulators of sulfur-responsive gene expression, respectively, alone or in combination, on the sulfur-responsive gene expression in ten-day-old Arabidopsis thaliana plants. We determined the relationship between the concentrations of GSH, OAS and sulfate, and expression of sulfur-responsive genes in rosette leaves. The concentrations of GSH and OAS correlated negatively and positively with expression of genes, respectively, suggesting that GSH and OAS independently affect expression of sulfur-responsive genes. A simple scheme of sulfur assimilation pathway from sulfate to glutathione. Sulfate in the environment is taken up and reduced to form cysteine (Cys). O-acetyl-L-serine (OAS) is synthesized from the N assimilation pathway and conjugate with reduced sulfur to form Cys. Glutathione (GSH) is synthesized from Cys and the synthesis is inhibited by buthionine sulfoximine (BSO). OAS and GSH have been shown to affect expression of sulfur-responsive genes.We examined the effects of exogenous application of GSH and OAS on the expression of sulfur-responsive genes or elements, b SR , Sultr2;2, APR1, and SAT1 in NOB7 plants. There was no significant difference in expression patterns of Sultr2;2, APR1, and SAT1 between the NOB7 plants and Col-0 wild-type plants in any of the following experiments (data not shown). Plants were initially grown on agarose plates containing 1,500 mM sulfate for ten days. Then they were cultured for four days on fresh agarose plates containing 1,500 mM or 1.5 mM sulfate with or without 0.3 mM GSH, 10 mM OAS or 1 mM BSO alone or in combination (Table 1). The media used in the present study were as described previously (Hirai et al. 1995). OAS, GSH and BSO were filter-sterilized and added after autoclaving the media to avoid possible degradation during autoclaving. To avoid any changes in pH in the media by addition of these reagents, we dissolved OAS, GSH and BSO in a small volume of media and readjusted the pH prior to filter sterilization.After the treatment, we collected the rosette leaves of the plants and determined the concentrations of GSH, OAS and sulfate, referred to hereafter as [GSH] (Table 2), and levels of GFP fluorescence, and accumulation of mRNA for Sultr2;2, APR1, and SAT1 (Table 3). For the quantification of GFP, we used relative GFP fluorescence (Table 3), which represents the intensity of fluorescence corresponding to GFP in NOB7 plants relative to chlorophyll autofluorescence as described by Niwa et al. (1999). The relative levels of Sultr2;2, APR1 or SAT1 transcript accumulation to that of b-tubulin transcript were caluculated (Table 3) (Table 2, compare C and CG). These results suggest that the negative effect of GSH on the expression of sulfur-responsive genes was not evident in plants grown with normal levels of sulfate. In these plants, [GSH] GSH, OAS and BSO were applied at the initial concentrations of 0.3, 10, and...

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Inter‐organ signaling in plants: regulation of ATP sulfurylase and sulfate transporter genes expression in roots mediated by phloem‐translocated compound

Cited by 269 publications

References 33 publications

Exogenous salicylic acid improves photosynthesis and growth through increase in ascorbate-glutathione metabolism and S assimilation in mustard under salt stress

Exogenous salicylic acid improves photosynthesis and growth through increase in ascorbate-glutathione metabolism and S assimilation in mustard under salt stress

Phloem-Localizing Sulfate Transporter, Sultr1;3, Mediates Re-Distribution of Sulfur from Source to Sink Organs in Arabidopsis

Independent roles of glutathione and O-acetyl-L-serine in regulation of sulfur-responsive gene expression in Arabidopsis thaliana

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