Inter-protein interactions govern protein loading into porous vaterite CaCO<sub>3</sub> crystals

Feoktistova, Natalia; Балабушевич, Н. Г.; Skirtach, André G.; Volodkin, Dmitry; Vikulina, Anna S.

doi:10.1039/d0cp00404a

Cited by 22 publications

(21 citation statements)

References 73 publications

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“…Together with mild vaterite decomposition conditions, this opportunity opens broader avenues for further utilization of such SERS platforms. Another important advantage of this platform is the ability to selectively locate probe macromolecules inside the microshell pores by pre-loading of the probes into the vaterite crystals; this is especially interesting, because mesopores of vaterite and those of Au microshells are in the same dimension as typical biomacromolecules [40]. Such a host-guest approach for SERS selectivity has several advantages in comparison, for example, with antibody-antigen binding-based techniques.…”

Section: Discussionmentioning

confidence: 99%

Mesoporous One-Component Gold Microshells as 3D SERS Substrates

et al. 2021

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Surface-enhanced Raman scattering (SERS) is a powerful analytical tool for label-free analysis that has found a broad spectrum of applications in material, chemical, and biomedical sciences. In recent years, a great interest has been witnessed in the rational design of SERS substrates to amplify Raman signals and optionally allow for the selective detection of analytes, which is especially essential and challenging for biomedical applications. In this study, hard templating of noble metals is proposed as a novel approach for the design of one-component tailor-made SERS platforms. Porous Au microparticles were fabricated via dual ex situ adsorption of Au nanoparticles and in situ reduction of HAuCl4 on mesoporous sacrificial microcrystals of vaterite CaCO3. Elimination of the microcrystals at mild conditions resulted in the formation of stable mesoporous one-component Au microshells. SERS performance of the microshells at very low 0.4 µW laser power was probed using rhodamine B and bovine serum albumin showing enhancement factors of 2 × 108 and 8 × 108, respectively. The proposed strategy opens broad avenues for the design and scalable fabrication of one-component porous metal particles that can serve as superior SERS platforms possessing both excellent plasmonic properties and the possibility of selective inclusion of analyte molecules and/or SERS nanotags for highly specific SERS analysis.

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Section: Discussionmentioning

confidence: 99%

Mesoporous One-Component Gold Microshells as 3D SERS Substrates

et al. 2021

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“…The core principle of the former synthesis method is the reaction of Ca 2+ and CO 3 2− ions in an aqueous solution to form insoluble precipitate, as shown in Figure 3 [ 53 ]. It has been identified that water [ 54 ], ions [ 55 ], and biomolecules [ 56 ] play an important role in the formation process of calcium carbonate. In regard with water, the mechanism of different routes shown in the Figure 3 is as follows: (i, ii) when solutions are undersaturated with respect to ACC but supersaturated with respect to any other crystalline CaCO 3 phases, the crystalline nuclei will bypass ACC and grow into larger crystals through diffusion and adsorption of Ca 2+ and CO 3 2− ions [ 57 ].…”

Section: Caco 3 Carriersmentioning

confidence: 99%

Hard, Soft, and Hard-and-Soft Drug Delivery Carriers Based on CaCO3 and Alginate Biomaterials: Synthesis, Properties, Pharmaceutical Applications

et al. 2022

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Because free therapeutic drug molecules often have adverse effects on normal tissues, deliver scanty drug concentrations and exhibit a potentially low efficacy at pathological sites, various drug carriers have been developed for preclinical and clinical trials. Their physicochemical and toxicological properties are the subject of extensive research. Inorganic calcium carbonate particles are promising candidates as drug delivery carriers owning to their hardness, porous internal structure, high surface area, distinctive pH-sensitivity, low degradability, etc, while soft organic alginate hydrogels are also widely used because of their special advantages such as a high hydration, bio-adhesiveness, and non-antigenicity. Here, we review these two distinct substances as well as hybrid structures encompassing both types of carriers. Methods of their synthesis, fundamental properties and mechanisms of formation, and their respective applications are described. Furthermore, we summarize and compare similarities versus differences taking into account unique advantages and disadvantages of these drug delivery carriers. Moreover, rational combination of both carrier types due to their performance complementarity (yin-&yang properties: in general, yin is referred to for definiteness as hard, and yang is broadly taken as soft) is proposed to be used in the so-called hybrid carriers endowing them with even more advanced properties envisioned to be attractive for designing new drug delivery systems.

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“…This approach allows for the encapsulation of various bioactive molecules at mild conditions with a controlled loading mechanism (e.g. proteins and enzymes as well as small drugs) [ 15 , 16 , 42 , 43 , 44 , 45 , 46 ] and demonstrates simple methods of release via external stimuli such as temperature and ionic strength [ 47 , 48 ] variations of which are relevant to biomedical applications such as implant coatings [ 49 , 50 ]. It is of note that bioactive molecules can be themselves a solid matrix treated by the layer-by-layer deposition, for instance, protein aggregates [ 51 , 52 ].…”

Section: Modification Of Various Surfaces With Vaterite Cacomentioning

confidence: 99%

Modification of Surfaces with Vaterite CaCO3 Particles

et al. 2022

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Former studies have demonstrated a strong interest toward the crystallization of CaCO3 polymorphs in solution. Nowadays, CaCO3 crystallization on solid surfaces is extensively being studied using biomolecules as substrates for the control of the growth aiming at various applications of CaCO3. Calcium carbonate exists in an amorphous state, as three anhydrous polymorphs (aragonite, calcite and vaterite), and as two hydrated polymorphs (monohydrocalcite and ikaite). The vaterite polymorph is considered as one of the most attractive forms due to its large surface area, biocompatibility, mesoporous nature, and other features. Based on physical or chemical immobilization approaches, vaterite can be grown directly on solid surfaces using various (bio)molecules, including synthetic polymers, biomacromolecules such as proteins and peptides, carbohydrates, fibers, extracellular matrix components, and even biological cells such as bacteria. Herein, the progress on the modification of solid surfaces by vaterite CaCO3 crystals is reviewed, focusing on main findings and the mechanism of vaterite growth initiated by various substances mentioned above, as well as the discussion of the applications of such modified surfaces.

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Inter-protein interactions govern protein loading into porous vaterite CaCO₃ crystals

Abstract: Loading of therapeutic proteins into mesoporous vaterite crystals is driven by inter-protein interactions in bulk solution and inside the crystals.

Cited by 22 publications

References 73 publications

Mesoporous One-Component Gold Microshells as 3D SERS Substrates

Mesoporous One-Component Gold Microshells as 3D SERS Substrates

Hard, Soft, and Hard-and-Soft Drug Delivery Carriers Based on CaCO3 and Alginate Biomaterials: Synthesis, Properties, Pharmaceutical Applications

Modification of Surfaces with Vaterite CaCO3 Particles

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Inter-protein interactions govern protein loading into porous vaterite CaCO3 crystals

Abstract: Loading of therapeutic proteins into mesoporous vaterite crystals is driven by inter-protein interactions in bulk solution and inside the crystals.

Cited by 22 publications

References 73 publications

Mesoporous One-Component Gold Microshells as 3D SERS Substrates

Mesoporous One-Component Gold Microshells as 3D SERS Substrates

Hard, Soft, and Hard-and-Soft Drug Delivery Carriers Based on CaCO3 and Alginate Biomaterials: Synthesis, Properties, Pharmaceutical Applications

Modification of Surfaces with Vaterite CaCO3 Particles

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Inter-protein interactions govern protein loading into porous vaterite CaCO₃ crystals