Inter simple sequence repeat (ISSR) based analysis of genetic diversity ofLobelia rhynchopetalum(Campanulaceae)

Abstract

An understanding of the patterns of genetic variation within and among populations of plant species is essential for devising optimum genetic management strategies for their conservation. Here, the inter simple sequence repeat (ISSR) technique was used to study genetic variation of the Afroalpine giant lobelia, Lobelia rhynchopetalum, based on ten populations sampled from Bale and Simen mountains in Ethiopia. The percentage of polymorphic loci across all samples (P(S)) and within population (Pp) was 78% and 27%, respectively. Regardless of a high total genetic variation, the species has quite low variation within populations. All genetic variation analyses revealed higher variation among populations than within populations (G'(ST)= 0.59, G(ST)=0.63, F(ST)=0.58). Analysis of molecular variance (AMOVA) that employed 156 ISSR markers revealed significant variations among populations; among the two mountain systems and among the three altitudinal groups (P < 0.001). The implications of these findings are discussed, especially from conservation point of view.

Population Genetic Analysis ofLobelia rhynchopetalumHemsl. (Campanulaceae) Using DNA Sequences fromITSand Eight Chloroplast DNA Regions

“…Two major clades supported by high bootstrap values (>90%) were formed, in which the haplotypes from the two mountain systems were clearly separated. The result is in agreement with the ISSR-based study [34] in grouping populations according to mountain system of origin. Generally, all analyses revealed a significant differentiation of L. rhynchopetalum populations at various hierarchical levels.…”

“…For in situ conservation, priority should be given to populations with relatively high genetic diversity. The results of this study and the ISSR-based study [34] are not in complete agreement as to which of these populations have high diversity, which makes it difficult to prioritize specific populations for in situ conservation. However, simultaneous consideration of the two data sets and environmental factors suggests Goba-1 and Debark-1 as good candidates for in situ conservation.…”

Population genetics of self-incompatibility and developing self-compatible genotypes in niger (Guizotia abyssinica)

“…To distinguish the F 1 hybrids from the parental and selfed plants, we extracted DNA from young leaves collected from 15-day-old G 3 plants using a modified CTAB procedure as described in Bekele et al (2007). Representative DNA samples were analyzed using inter-simple sequence repeat (ISSR) molecular marker techniques as described in Geleta and Bryngelsson (2009).…”

Identification and characterisation of functional expressed sequence tags-derived simple sequence repeat (eSSR) markers for genetic linkage mapping of Schistosoma mansoni juvenile resistance and susceptibility loci in Biomphalaria glabrata

“…However, primers were successfully designed from 33 ESTs. Primers from these sequences, including 46 primers previously designed from gSSRs (Wake and Vredenburg, 2008), and the set of six ISSR primers (Hashizume et al, 2003; Geleta and Bryngelsson, 2009) were used for the marker transferability test.…”

“…However, it proved to be difficult or impossible to design primers for most of the Bg -eSSRs since potential priming sites were inconveniently located either at the border of the EST or surrounded by excessive AT-rich sequences. Another two groups of primers were included: (i) genomic microsatellite primers that were shown previously to differentiate between resistant and susceptible snails (Wake and Vredenburg, 2008) and (ii) and ISSR primers previously designed and identified with high frequency from Bg -eSSRs (Geleta and Bryngelsson, 2009). The major parameters for Bg -eSSR primer design were set as follows: expected size of PCR product, between 100 and 350 bp; optimum annealing temperature between 57 and 60 °C so all PCR amplifications could be conveniently achieved at the same annealing temperature.…”