Inter simple sequence repeats separate efficiently hemp from marijuana (Cannabis sativa L.)

Hakkı, Erdoğan E.; Kayış, Seyit Ali; Pinarkara, Emine; Sag, Ayla

doi:10.2225/vol10-issue4-fulltext-4

Cited by 18 publications

(12 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ISSR primers generated a total of 157 with an average of 8 bands per primer, out of which 63 bands were monomorphic. Hakki et al (2007) also reported effective segregation of species with ISSR. Kojoma et al (2002) also used ISSR for studying DNA finger printing in C. sativa.…”

Section: Resultsmentioning

confidence: 94%

Comparative effectiveness of inter-simple sequence repeat and randomly amplified polymorphic DNA markers to study genetic diversity of Indian Garcinia

Parthasarathy¹,

Nandakishore²,

Babu³

et al. 2013

Afr. J. Biotechnol.

View full text Add to dashboard Cite

A study to compare the effectiveness of inter-simple sequence repeats (ISSR) and randomly amplified polymorphic DNA (RAPD) profiling was carried out with a total of 65 DNA samples using 12 species of Indian Garcinia. ISSR and RAPD profiling were performed with 19 and 12 primers, respectively. ISSR markers generated a total number of 156 bands with 92 polymorphic bands, while RAPD markers produced a total of 134 bands with 80 polymorphic bands. Percentage of polymorphic loci in RAPD profiling was 60.4% while in ISSR profiling, it was 59.3%. Heterogeneity index was similar for the markers, 0.86 for ISSR and 0.89 for RAPD, indicating that both the marker systems are effective in determining polymorphism in Garcinia. ISSR markers showed clear distinction among the species whereas RAPD markers showed segregation based on geographical location as well as species based.

show abstract

Section: Resultsmentioning

confidence: 94%

Comparative effectiveness of inter-simple sequence repeat and randomly amplified polymorphic DNA markers to study genetic diversity of Indian Garcinia

Parthasarathy¹,

Nandakishore²,

Babu³

et al. 2013

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…This was in agreement with Pradeep et al (2002) who found that ISSR markers are highly polymorphic and are useful in studies on genetic diversity, phylogeny, genome mapping and evolutionary biology. Hakki et al (2007) also reported effective segregation of species with ISSR. The results obtained in this study Figure 3: Scan of PAGE-electrophoretic patterns of proteins of Alex (by TotalLab Quant ® program, 2013).…”

Section: Discussionmentioning

confidence: 94%

Use of inter simple sequence repeats and protein markers in assessing genetic diversity and relationships among four rabbit genotypes

El-Sabrout

Aggag

2015

World Rabbit Sci.

View full text Add to dashboard Cite

The importance of DNA and protein applications as powerful tools in breeding programmes is revealed. The inter simple sequence repeats (ISSR) technique was used to characterise and determine phylogenetic relationships among 4 genotypes of rabbit, namely Alexandria (Alex), V line (V), New Zealand White (NZW) and California (Cal). Six out of 7 ISSR primers exhibited sufficient variability and were used to characterise the genetic diversity and relationships among studied genotypes. A total of 141 DNA bands were detected. DNA fragments were generated with 87 (61.7%) being polymorphic, indicating considerable genetic variation among the examined genotypes. While protein electrophoresis provides a precise method for assaying variation in serum proteins which play an important part in productive performance. The results demonstrated 2 specific protein markers in Alexandria rabbits; these specific protein markers may be responsible for the superiority of Alexandria line in weight. Phylogenetic analysis based on Nei and Li unbiased genetic distance illustrated that (Alex & V) and (Cal & V) were genetically closely related. Our results showed that ISSR and protein electrophoresis are useful methods to detect different genetic expressions and understand the variability in some productive traits in rabbits.

show abstract

“…Some of the materials, used in this study, were previously also utilized for discriminating drug type Cannabis from hemp types (Hakki et al 2007). All the information relevant to the seized samples and accessions are shown in Table 1.…”

Section: Plant Materialsmentioning

confidence: 99%

“…In a preliminary work conducted with three strains of C. sativa from different sources, Kojoma et al (2002) reported that different samples were identified by means of inter simple sequence repeats (ISSR). In a recent study by using ISSRs marijuana (Cannabis sativa L.) was separated efficiently from hemp (Hakki et al 2007). Gillan et al (1995) reported the differentiation of C. sativa samples with the use of RAPDs when HPLC analysis was inefficient.…”

mentioning

confidence: 99%

RAPD analysis of seized marijuana (Cannabis sativa L.) in Turkey

Pinarkara¹,

Kayış²,

Hakkı³

et al. 2009

Electron. J. Biotechnol.

Self Cite

View full text Add to dashboard Cite

Inter simple sequence repeats separate efficiently hemp from marijuana (Cannabis sativa L.)

Cited by 18 publications

References 20 publications

Comparative effectiveness of inter-simple sequence repeat and randomly amplified polymorphic DNA markers to study genetic diversity of Indian Garcinia

Comparative effectiveness of inter-simple sequence repeat and randomly amplified polymorphic DNA markers to study genetic diversity of Indian Garcinia

Use of inter simple sequence repeats and protein markers in assessing genetic diversity and relationships among four rabbit genotypes

RAPD analysis of seized marijuana (Cannabis sativa L.) in Turkey

Contact Info

Product

Resources

About