Interacting active surfaces: a model for three-dimensional cell aggregates

Torres-Sánchez, Alejandro; Winter, Max Kerr; Salbreux, Guillaume

doi:10.1101/2022.03.21.484343

Cited by 5 publications

(4 citation statements)

References 71 publications

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“…According to this model, a decrease in cell polarity would lead to a reduced ability to form strong actin fibres and to adapt to a lower-stiffness substrate surface. Mathematical models also suggest that stronger intercellular adhesion makes it easier for colonies to aggregate and form a circle [ 39 ]. In the present study, the decreased expression of intercellular adhesion factors was observed in hiPSCs cultured on iMatrix-221 but not on iMatrix-511.…”

Section: Resultsmentioning

confidence: 99%

Semi-3D cultures using Laminin 221 as a coating material for human induced pluripotent stem cells

Nakashima

Yoshida

Tsukahara

2022

Regenerative Biomaterials

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It was previously believed that human induced pluripotent stem cells (hiPSCs) did not show adhesion to the coating material laminin 221, which is known to have specific affinity for cardiomyocytes. In this study, we report that human mononuclear cell-derived hiPSCs, established with Sendai virus vector, form peninsular-like colonies rather than embryonic stem cell-like colonies; these peninsular-like colonies can be passaged more than 10 times after establishment. Additionally, initialization-deficient cells with residual Sendai virus vector adhered to the coating material Laminin 511 but not to Laminin 221. Therefore, the expression of undifferentiated markers tended to be higher in hiPSCs established on Laminin 221 than on Laminin 511. On Laminin 221, hiPSCs15M66 showed a semi-floating colony morphology. The expression of various markers of cell polarity was significantly lower in hiPSCs cultured on Laminin 221 than in hiPSCs cultured on Laminin 511. Furthermore, 201B7 and 15M66 hiPSCs showed three-dimensional cardiomyocyte differentiation on Laminin 221. Thus, the coating material Laminin 221 provides semi-floating culture conditions for the establishment, culture, and induced differentiation of hiPSCs.

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Section: Resultsmentioning

confidence: 99%

Semi-3D cultures using Laminin 221 as a coating material for human induced pluripotent stem cells

Nakashima

Yoshida

Tsukahara

2022

Regenerative Biomaterials

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“…Furthermore, in a more general sense, SEM might be regarded as a class of models which describe the cellular body or intracellular structures of each cell, such as a cell surface (membrane and cortex) and a nucleus, by a certain meshwork of many nodes. This might include the model relying on the interconnected particles representing subcellular structures [29,30] and the continuous descriptions of cell surfaces implemented by triangularmesh discretizations or some finite-element discretization [154][155][156].…”

Section: Experimental Readouts For Comparisonmentioning

confidence: 99%

Computational approaches for simulating luminogenesis

Fuji¹,

Tanida²,

Sano³

et al. 2022

Seminars in Cell & Developmental Biology

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“…6a-c, Supplementary Information section 5.4). Simulations were performed using the Interacting Active Surfaces (IAS) numerical framework 17 (Fig. 4d, Supplementary Information, sections 7.1 and 7.2).…”

Section: An Active Surface Model Recapitulates Doublet Rotationmentioning

confidence: 99%

Polarity-driven three-dimensional spontaneous rotation of a cell doublet

Guyomar

Vagne

et al. 2022

Preprint

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Cell mechanical interactions play a fundamental role in the self-organisation of organisms. How these interactions drive coordinated cell movement in three-dimensions remains unclear. Here we report that cell doublets embedded in a 3D extracellular matrix undergo spontaneous rotations and we investigate the rotation mechanism using live cell imaging, quantitative measurements, mechanical perturbations, and theory. We find that rotation is driven by a polarized distribution of myosin within cell cortices. The mismatched orientation of this polarized distribution breaks the doublet mirror symmetry. In addition, cells adhere at their interface through adherens junctions and with the extracellular matrix through focal contacts near myosin clusters. Using a physical theory describing the doublet as two interacting active surfaces, we find that rotation is driven by myosin-generated gradients of active tension, whose profiles are dictated by interacting cell polarity axes. We show that interface three-dimensional shapes can be understood from the Curie principle: shapes symmetries are related to broken symmetries of myosin distribution in cortices. To test for the rotation mechanism, we suppress myosin clusters using laser ablation and we generate new myosin clusters by optogenetics. Our work clarifies how polarity-oriented active mechanical forces drive collective cell motion in three dimensions.

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Interacting active surfaces: a model for three-dimensional cell aggregates

Cited by 5 publications

References 71 publications

Semi-3D cultures using Laminin 221 as a coating material for human induced pluripotent stem cells

Semi-3D cultures using Laminin 221 as a coating material for human induced pluripotent stem cells

Computational approaches for simulating luminogenesis

Polarity-driven three-dimensional spontaneous rotation of a cell doublet

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