Interaction between 6/94 virus, a parainfluenza type 1 strain, and mouse macrophages

Verini, M.A.; Lief, Florence S.

doi:10.1128/iai.24.3.720-728.1979

Cited by 3 publications

(11 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Susceptibility of lymphocytes from immunized mice to infection. The immunization of mice with 6/94 virus and their humoral antibody responses as well as the susceptibility of their macrophages to infection with the same virus have been reported (11). Lymphocytes from the same immunized and normal mice were infected with 6/94 egg-grown virus.…”

Section: Resultsmentioning

confidence: 98%

“…Infection was carried out by incubating the cellvirus mixtures at 370C for 1 to 2 h and discarding the unadsorbed inoculum by a series of centrifugations and washings in phosphate-buffered saline, so that less than 10' TCID50 (50% tissue culture infective dose) was calculated to remain in the last supernatant fluid. In some experiments, residual virus was neutralized by incubating the washed inoculated cells with specific antiserum as described for macrophage infections (11). The dosage of virus used for inoculation varied with the experiments.…”

Section: Methodsmentioning

confidence: 99%

“…(vi) Viral assay. Hemagglutination titers and infectious virus (TCID50) production were measured as described previously for macrophage cultures (11). The total production of infectious virus by lymphocytes was always determined by conducting titrations of sonicated suspensions of the infected cells.…”

Section: Methodsmentioning

confidence: 99%

“…immunization of C3H mice and assays for development of humoral antibodies were as reported in the previous paper (11).…”

Section: Infect Immunmentioning

confidence: 99%

See 3 more Smart Citations

Interaction between 6/94 virus, a parainfluenza type 1 strain, and unstimulated mouse lymphocytes

Verini¹,

Lief²

1979

Infect Immun

Self Cite

View full text Add to dashboard Cite

6/94 virus was found to grow in unstimulated splenic mouse lymphocytes depleted of monocyte macrophages. Both egg-grown virus and virus produced in mouse macrophage cultures induced the development of hemadsorption and the appearance of new infectious virus in the lymphocyte cultures. An antigenic relative. Sendai virus, on the contrary, was quickly inactivated in these lymphocyte cultures, in agreement with previous reports in the literature. The T lymphocyte population seemed to be the fraction principally involved in the replication of 6/94 virus. The immunization of mice with 6/94 virus and the appearance of high levels of humoral neutralizing antibodies did not inhibit completely the susceptibility of their lymphocytes to the infectious agent.

show abstract

Section: Resultsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…immunization of C3H mice and assays for development of humoral antibodies were as reported in the previous paper (11).…”

Section: Infect Immunmentioning

confidence: 99%

See 2 more Smart Citations

Interaction between 6/94 virus, a parainfluenza type 1 strain, and unstimulated mouse lymphocytes

Verini¹,

Lief²

1979

Infect Immun

Self Cite

View full text Add to dashboard Cite

show abstract

“…The assumption could be made, therefore, that the variability of results in our experiments was due to unavoidable contamination of lymphocytes which produced cross-reacting, non-neutralizing antibodies. On the other hand, reports have been presented showing that multiplication of viruses, such as vaccinia (24), Semliki Forest (28), influenza (27), and parainfluenza (30) viruses, is suppressed in macrophage cultures from immunized animals. The discrepancies may be due to differences in the virus species used, or more likely, to differences in methods of immunization, in particular, homotypic or heterotypic.…”

Section: Discussionmentioning

confidence: 99%

Dengue Virus Multiplication in Cultures of Mouse Peritoneal Macrophages: Effects of Macrophage Activators

Hotta

1982

Microbiology and Immunology

View full text Add to dashboard Cite

Dengue-2 virus multiplied in cultures of methylcellulose-induced peritoneal macrophages of BALBjc mice. The in vitro-cultivated macrophages from dengue-I virus-immune mice produced larger amounts of dengue-2 virus than did those from nonimmune controls. The effect of macrophage activators was examined by using nonimmune macrophages. Enhanced virus production was demonstrated in cultures of macrophages pretreated with phytohemagglutinin (PHA) or bacterial lipopolysaccharide (LPS). The number of virus-infected cells in the pretreated cultures was estimated to be about 0.01 % or less of the total macrophages. Continuous treatment of macrophages with PHA before and after virus inoculation brought about the most marked enhancement of dengue-2 virus multiplication. On the other hand, treatment with concanavalin A or pokeweed mitogen showed little effect on the multiplication of the same virus. Treatment with carrageenan, a specific macrophage blocking agent, markedly suppressed dengue-2 virus production in both dengue-I virus-immune macrophages and LPStreated macrophages. The indirect fluorescent-antibody (FA) technique revealed dengue-2 viral antigen in the cytoplasm of infected macrophages, and the FApositive macrophages were more numerous in PHA-treated cultures than in untreated controls. The results obtained are discussed in relation to a possible role of activated monocytesjmacrophages in the pathogenesis of dengue hemorrhagic fever.

show abstract

Interaction between 6/94 virus, a parainfluenza type 1 strain, and human leukocytes

Verini¹,

Lief²

1979

Infect Immun

Self Cite

View full text Add to dashboard Cite

/94 virus, a parainfluenza type 1 virus recovered by lysolecithin fusion of multiple sclerosis brain cell cultures with CV-1 cells, replicated in monocyte macrophages and lymphocytes from normal human donors and from a patient with multiple sclerosis. In macrophage cultures, hemadsorption-positive cells and high levels of infectious virus became apparent within 24 to 48 h after infection, persisted for 6 days, and then began to decrease. Phytohemagglutinin-stimulated macrophages yielded similar titers of virus, but the levels were maintained for a longer period of time. Macrophage-produced virus appeared to be infectious for other macrophages in the same culture. Both unstimulated and phytohemagglutinin-stimulated lymphocytes also supported virus replication. Significantly higher titers were produced in the stimulated cultures, T cell-enriched populations producing more virus than unseparated populations whether stimulated or unstimulated. The presence or absence of antibodies to the virus in the donors did not appear to influence the levels of virus obtained in any of the leukocyte cultures. However, an increase in blastic forms after 6/94 virus infection was noted in lymphocytes from donors with antibodies as revealed morphologically and by increased incorporations of tritiated thymidine. Furthermore, 6/94 virusinfected lymphocytes, unlike Sendai virus-infected lymphocytes, were able to respond well to mitogenic stimulation by phytohemagglutinin. Two preceding papers (22, 23) described studies on the interaction of mouse macrophages and lymphocytes with 6/94 virus, an agent recovered from brain cell-derived cultures from two patients with multiple sclerosis (MS) after lysolecithin-mediated fusion with CV-1 cells (20). The results of these studies indicated that the virus replicated in both macrophages and unstimulated lymphocytes. Of interest was the finding that macrophages and lymphocytes derived from immunized mice that had high levels of specific humoral neutralizing antibodies were still variably susceptible to 6/94 infection. Zisman and Denman (26) reported that Sendai virus, an antigenic relative of the 6/94 agent (13), was inactivated by human and mouse lymphocytes independent of the presence of humoral antibodies. Differences in infectivity between 6/94 and Sendai viruses for mouse macrophages (4, 22) and lymphocytes (23, 26) made it of interest to investigate the interaction of 6/94 virus with human leukocytes.

show abstract

Interaction between 6/94 virus, a parainfluenza type 1 strain, and mouse macrophages

Cited by 3 publications

References 24 publications

Interaction between 6/94 virus, a parainfluenza type 1 strain, and unstimulated mouse lymphocytes

Interaction between 6/94 virus, a parainfluenza type 1 strain, and unstimulated mouse lymphocytes

Dengue Virus Multiplication in Cultures of Mouse Peritoneal Macrophages: Effects of Macrophage Activators

Interaction between 6/94 virus, a parainfluenza type 1 strain, and human leukocytes

Contact Info

Product

Resources

About