Interaction between Acidic Polysaccharides and Proteins

Biocatalyst and Colorimetric/Fluorescent Dual Biosensors of H2O2 Constructed via Hemoglobin–Cu3(PO4)2 Organic/Inorganic Hybrid Nanoflowers

“…Subsequently, the four subunits were dissociated from each other, and then they can stretch into loose peptide chains . It is known that the pI point of Hb is 6.7, which is lower than the pH value of the incubation system . Therefore, the protein molecules were negatively charged and can form complexes with Cu 2+ through coordination interactions of −COO – and amide groups in the Hb backbone .…”

“…39 It is known that the pI point of Hb is 6.7, which is lower than the pH value of the incubation system. 40 Therefore, the protein molecules were negatively charged and can form complexes with Cu 2+ through coordination interactions of −COO − and amide groups in the Hb backbone. 41 Thus, these chains are Cu 2+ /Hb complexes and provided the growth sites for nucleation of the primary crystals.…”

Conformational Changes in ι- and κ-Carrageenans Induced by Complex Formation with Bovine β-Casein

“…Information about conformational changes in protein−polyelectrolyte complexes in dilute solutions is limited. Formation of complexes with unstructured anionic polysaccharides decreases the conformational stability of some globular proteins. , Complex formation ,− and phase separation ,,− in mixtures of helix-forming polysaccharides and proteins, as well as rheology ,,,, and gelation , of these mixtures, have been intensively studied. Nevertheless, conformational changes in ordered polysaccharides induced by complex formation with proteins have not been investigated despite their potential use as models of the nonspecific interactions between double stranded DNA, proteins, and amphiphilic polymers.…”

Highly sensitive electrochemical detection of genomic DNA based on stem loop probes structured for magnetic collection and measurement via metalised hollow polyelectrolyte shells

“…The anti-DIG antibody was a fab fragment labeled with alkaline phosphatase (AP). The enzyme enabled adsorption to the positively charged APTS-coated Fe 3 O 4 by electrostatic attraction (AP PI = 4.5, Tadera et al, 2003) and could be used to test for successful connection to magnetic latex by colorimetric observation of p-nitrophenol, following AP-catalysed dephosphorylation of the substrate p-nitrophenyl phosphate (pNPP). We found that the characteristic yellow colour of p-nitrophenol was clearly observed when exposing anti-DIG modified magnetic latex to pNPP, but not in the absence of either anti-DIG or pNPP (see Supporting Information Fig.…”