Interaction between C18 fatty acids and DOPE PEG2000 in Langmuir monolayers: effect of degree of unsaturation

Gew, Lai Ti; Misran, Misni

doi:10.1007/s10867-017-9459-2

Cited by 9 publications

(10 citation statements)

References 48 publications

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“…The estimated surface area is in good agreement with the extra contribution found in the lost molecular area. Although PEG is usually regarded as inert, it is known to interact with lipid monolayers at low π especially when conjugated to a membrane constituent. − The observed single peaks in the C S /π graph show the simultaneous squeeze out of both spacer and peptide, which demonstrates the interdependent behavior of both during the squeeze out process.…”

Section: Resultsmentioning

confidence: 97%

Influence of Membrane–Fusogen Distance on the Secondary Structure of Fusogenic Coiled Coil Peptides

Daudey¹,

Schwieger

Rabe

et al. 2019

Langmuir

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Liposomal membrane fusion is an important tool to study complex biological fusion mechanisms. We use lipidated derivatives of the specific heterodimeric coiled coil pair E: (EIAALEK)3 and K: (KIAALKE)3 to study and control the fusion of liposomes. In this model system, peptides are tethered to their liposomes via a poly(ethylene glycol) (PEG) spacer and a lipid anchor. The efficiency of the fusion mechanism and function of the peptides is highly affected by the PEG-spacer length and the lipid anchor type. Here, the influence of membrane–fusogen distance on the peptide–membrane interactions and the peptide secondary structures is studied with Langmuir film balance and infrared reflection absorption spectroscopy. We found that the introduction of a spacer to monolayer-tethered peptide E changes its conformation from solvated random coils to homo-oligomers. In contrast, the described peptide–monolayer interaction of peptide K is not affected by the PEG-spacer length. Furthermore, the coexistence of different conformations when both lipopeptides E and K are present at the membrane surface is demonstrated empirically, which has many implications for the design of effective fusogenic recognition units and the field of artificial membrane fusion.

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Section: Resultsmentioning

confidence: 97%

Influence of Membrane–Fusogen Distance on the Secondary Structure of Fusogenic Coiled Coil Peptides

Daudey¹,

Schwieger

Rabe

et al. 2019

Langmuir

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“…This effect is followed by the growth of the fluidity with further addition of 2OHOA. Because 2OHOA has a cis double bond, bending the hydrocarbon residue, and it is well known that the presence of unsaturated fatty acid molecules has a fluidizing effect on lipid membranes, − it could be expected that the incorporation of its molecules into the EPOPC monolayer should induce acyl chain disordering and film expansion. On the other hand, the 2OHOA molecule possesses a carboxylic group undergoing dissociation and becoming negatively charged in the aqueous solution.…”

Section: Discussionmentioning

confidence: 99%

Studies on the Interactions of 2-Hydroxyoleic Acid with Monolayers and Bilayers Containing Cationic Lipid: Searching for the Formulations for More Efficient Drug Delivery to Cancer Cells

et al. 2019

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Drug delivery in cationic liposomes seems to be a promising therapeutic approach in cancer treatment. The rational design of the positively charged lipid vesicles as anticancer drug carriers should be supported by a detailed analysis of the interactions of the carrier components with anticancer drugs. In the present work, 2-hydroxyoleic acid (2OHOA; Minerval), a membrane lipid therapy drug, was incorporated into positively charged mono- and bilayer membranes containing 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine (EPOPC), the synthetic cationic lipid, and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). The intermolecular interactions, fluidity, and miscibility of the studied monolayers were analyzed by utilizing Langmuir balance experiments. The morphology of two-dimensional films was inspected using a Brewster angle microscopy technique. The properties of the liposomes were investigated by dynamic light scattering (DLS) and zeta potential measurements, steady-state fluorescence anisotropy experiments, and the spectrofluorimetric titration of calcein-encapsulated vesicles with a lysis-inducing agent. According to the collected results, 2OHOA intercalation into films of pure phospholipids or a binary EPOPC/DOPC film is thermodynamically favorable. Surprisingly, no significant effect of the presence of unsaturated 2OHOA chains on the EPOPC/DOPC monolayer order was observed. The experiments carried out for 2OHOA-inserted cationic EPOPC/DOPC (1:4) liposomes indicate effective incorporation of the drug into the liposome bilayer and the formation of stable vesicles without affecting their properties markedly. On the basis of the obtained results, EPOPC/DOPC/2OHOA cationic liposomes with 15% 2OHOA content in the phospholipid bilayer seem to be the most suitable for potential biomedical applications.

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“…The surface pressure of the LB films was measured by a computer-controlled Langmuir balance (KSV 5000, Langmuir System, Helsinki, Finland) equipped Teflon trough (48840 mm 2 trough area) with symmetric barriers was used to obtain the Π−A isotherms. The obtained Π−A isotherms were analyzed quantitatively by the compression modulus Cs -1 , excess mean molecular area, Aex, and the total Gibbs free energy of mixing ∆𝐺𝐺 𝑚𝑚𝑚𝑚𝑚𝑚 which we had described in our previous publications [4,5].…”

Section: Methodsmentioning

confidence: 99%

Mixed Langmuir Monolayers of C18 Fatty Acids: Effect of Degree of Saturation

Gew¹,

Misran

2020

MSF

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The degree of unsaturation of hydrocarbon chains in the lipid diversity of biological membrane will greatly influence the membrane protein function. Lipid-lipid interactions in the membrane domain are crucial in the development of novel liposomal nanocarrier as the stability and function of liposomes are important points to be considered. The embedded therapeutic proteins are dependent on lipid membrane composition and their physical properties. In this study, we elucidated lipid-lipid interactions in the biological membrane domain with energetic quantitative data by mixing any two C18 fatty acids namely, stearic acid (SA), oleic acid (L1), linoleic acid (L2), and linolenic acid (L3) in Langmuir-Blodgett trough with water subphase. In general, SA has a saturated hydrocarbon chain; meanwhile, L1, L2 and L3 have increasing degree of unsaturation in their respective hydrocarbon chain. The tail-to-tail interactions were studied by the combinations of SA/L1, SA/L2, SA/L3, L1/L2, L1/L3 and L2/L3 at various mole ratios ranging from 9:1, 8:2, 7:3 ... to 1:9. Among the mixtures of C18 fatty acids, L2/L3 is considered as the energetically stable at the optimum amount of XL2 and XL3 = 0.5 respectively, at all discrete surface pressure.

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Interaction between C18 fatty acids and DOPE PEG2000 in Langmuir monolayers: effect of degree of unsaturation

Cited by 9 publications

References 48 publications

Influence of Membrane–Fusogen Distance on the Secondary Structure of Fusogenic Coiled Coil Peptides

Influence of Membrane–Fusogen Distance on the Secondary Structure of Fusogenic Coiled Coil Peptides

Studies on the Interactions of 2-Hydroxyoleic Acid with Monolayers and Bilayers Containing Cationic Lipid: Searching for the Formulations for More Efficient Drug Delivery to Cancer Cells

Mixed Langmuir Monolayers of C18 Fatty Acids: Effect of Degree of Saturation

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