Interaction between Campylobacter and intestinal epithelial cells leads to a different proinflammatory response in human and porcine host

Aguilar, Carmen; Jiménez-Marín, Ángeles; Martins, Rodrigo Prado; Garrido, Juan J.

doi:10.1016/j.vetimm.2014.09.003

Cited by 13 publications

(18 citation statements)

References 56 publications

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“…eIF2 signaling, critical for stress-induced regulation of translation in eukaryotic cells, is activated by pathogens and is part of a general antibacterial defense system (Shrestha et al, 2012). In agreement with previous studies (Skjolaas et al, 2007; Horrocks et al, 2009; Bratz et al, 2013; Aguilar et al, 2014), we found just a slight inflammatory response in porcine intestinal cells after Campylobacter infection, indicating a potential commensal behavior.…”

Section: Discussionsupporting

confidence: 93%

“…Much less is known about why Campylobacter is pathogenic to humans but acts as commensal bacterium in the gastrointestinal tract of pigs (Horrocks et al, 2009; Bratz et al, 2013). In a previous work we demonstrated that C. jejuni adheres preferentially to human intestinal epithelial cells and that this cellular tropism was accompanied by a strong inflammatory response (Aguilar et al, 2014). In this work, using a next generation proteomic approach, we sought to understand better the duality of behavior from commensal to pathogen that Campylobacter manifests in the porcine and human host, respectively.…”

Section: Discussionmentioning

confidence: 87%

“…The optical density (OD 600 ) was adjusted to 1 to achieve 10 8 CFU/ml for their straight inoculation into INT-407 and IPEC-1 cells at a multiplicity of infection (MOI) of 100/1. All in vitro cell infections with bacteria were performed in triplicate as previously described (Aguilar et al, 2014) using two points in the study time course: 3 h (early time, T3h) and 24 h (late time, T24h). The same time course was used with the uninfected controls.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, little is known about the mechanisms that determine the pathogenic of commensal character of Campylobacter in different hosts. In a previous in vitro study, we revealed that C. jejuni preferentially interacted with human intestinal epithelial cells in which the level of bacterial invasion was up to 10-fold higher than in porcine intestinal cells (Aguilar et al, 2014). This study also showed that a strong inflammatory response occurred in human cells after bacterial infection while no response was observed in intestinal epithelial cells of porcine origin.…”

Section: Introductionmentioning

confidence: 92%

“…usually colonizes the gut of chickens and pigs, where it seems to establish a commensal relationship, harboring concentrations of 10 7 –10 10 CFU/gram of feces in their intestines but lacking the development of clinical signs in the host (Battersby et al, 2016). Despite the importance of the disease in humans, our understanding of the virulence mechanisms of Campylobacter is still relatively poor (Dasti et al, 2010; Aguilar et al, 2014). Moreover, little is known about the mechanisms that determine the pathogenic of commensal character of Campylobacter in different hosts.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Proteomics Reveals Differences in Host-Pathogen Interaction between Infectious and Commensal Relationship with Campylobacter jejuni

Ayllón

Jiménez-Marín

Argüello

et al. 2017

Front. Cell. Infect. Microbiol.

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Campylobacter jejuni is the leading food-borne poisoning in industrialized countries. While the bacteria causes disease in humans, it merely colonizes the gut in poultry or pigs, where seems to establish a commensal relationship. Until now, few studies have been conducted to elucidate the relationship between C. jejuni and its different hosts. In this work, a comparative proteomics approach was used to identify the underlying mechanisms involved in the divergent outcome following C. jejuni infection in human and porcine host. Human (INT-407) and porcine (IPEC-1) intestinal cell lines were infected by C. jejuni for 3 h (T3h) and 24 h (T24h). C. jejuni infection prompted an intense inflammatory response at T3h in human intestinal cells, mainly characterized by expression of proteins involved in cell spreading, cell migration and promotion of reactive oxygen species (ROS). Proteomic analysis evidenced significantly regulated biofunctions in human cells related with engulfment and endocytosis, and supported by canonical pathways associated to infection such as caveolar- and clathrin-mediated endocytosis signaling. In porcine IPEC-1 cells, inflammatory response as well as signaling pathways that control cellular functions such as cell migration, endocytosis and cell cycle progression resulted downregulated. These differences in the host response to infection were supported by the different pattern of adhesion and invasion proteins expressed by C. jejuni in human and porcine cells. No marked differences in expression of virulence factors involved in adaptive response and iron acquisition functions were observed. Therefore, the results of this study suggest that both host and pathogen factors are responsible for commensal or infectious character of C. jejuni in different hosts.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comparative Proteomics Reveals Differences in Host-Pathogen Interaction between Infectious and Commensal Relationship with Campylobacter jejuni

Ayllón

Jiménez-Marín

Argüello

et al. 2017

Front. Cell. Infect. Microbiol.

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Campylobacter sp.: Pathogenicity factors and prevention methods—new molecular targets for innovative antivirulence drugs?

Kreling

Falcone

Kehrenberg

et al. 2020

Appl Microbiol Biotechnol

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Infections caused by bacterial species from the genus Campylobacter are one of the four main causes of strong diarrheal enteritis worldwide. Campylobacteriosis, a typical food-borne disease, can range from mild symptoms to fatal illness. About 550 million people worldwide suffer from campylobacteriosis and lethality is about 33 million p.a. This review summarizes the state of the current knowledge on Campylobacter with focus on its specific virulence factors. Using this knowledge, multifactorial prevention strategies can be implemented to reduce the prevalence of Campylobacter in the food chain. In particular, antiadhesive strategies with specific adhesion inhibitors seem to be a promising concept for reducing Campylobacter bacterial load in poultry production. Antivirulence compounds against bacterial adhesion to and/or invasion into the host cells can open new fields for innovative antibacterial agents. Influencing chemotaxis, biofilm formation, quorum sensing, secretion systems, or toxins by specific inhibitors can help to reduce virulence of the bacterium. In addition, the unusual glycosylation of the bacterium, being a prerequisite for effective phase variation and adaption to different hosts, is yet an unexplored target for combating Campylobacter sp. Plant extracts are widely used remedies in developing countries to combat infections with Campylobacter. Therefore, the present review summarizes the use of natural products against the bacterium in an attempt to stimulate innovative research concepts on the manifold still open questions behind Campylobacter towards improved treatment and sanitation of animal vectors, treatment of infected patients, and new strategies for prevention. Key points • Campylobacter sp. is a main cause of strong enteritis worldwide. • Main virulence factors: cytolethal distending toxin, adhesion proteins, invasion machinery. • Strong need for development of antivirulence compounds.

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Mechanisms of gastrointestinal pathogenesis and landscape of intestinal immunity

Samanta¹

2023

Viral, Parasitic, Bacterial, and Fungal Infections

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Interaction between Campylobacter and intestinal epithelial cells leads to a different proinflammatory response in human and porcine host

Cited by 13 publications

References 56 publications

Comparative Proteomics Reveals Differences in Host-Pathogen Interaction between Infectious and Commensal Relationship with Campylobacter jejuni

Comparative Proteomics Reveals Differences in Host-Pathogen Interaction between Infectious and Commensal Relationship with Campylobacter jejuni

Campylobacter sp.: Pathogenicity factors and prevention methods—new molecular targets for innovative antivirulence drugs?

Mechanisms of gastrointestinal pathogenesis and landscape of intestinal immunity

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