Interaction between CD44 and highly condensed hyaluronic acid through crosslinking with proteins

Modification of the antigenicity of cancer cells by conjugates consisting of hyaluronic acid and foreign antigens

“…In our previous study, the addition of HA to cells uncoated with rCD44 did not induce a decrease in the QCM frequency. 21 Therefore, the frequency decreases observed in this study also reflect the specific binding of HA to rCD44. Although 100k1HA-1OVA showed a slightly larger decrease than 100kHA, this was due to it containing an OVA protein and having a heavier weight per molecule than 100kHA.…”

“…In our previous study, a conjugate containing multiple HA (80kHA) and BSA molecules and adopting a highly condensed structure was recognized by the rCD44 molecule more than 80kHA and was internalized into CT 26 cells with high efficiency. 21 In this study, 100k4HA-3OVA was well recognized by the rCD44 molecule and the cells expressing CD44, compared with the recognition levels for 100kHA and 100k1HA-1OVA. These results suggest that CD44 prefers HA with a condensed structure.…”

“…2B). 21 100kHA and 500kHA are fitted by a straight line with slopes of 0.52 and 0.62, respectively, which correspond to a random coil swollen by the excluded volume effect. After conjugation with OVA, the slopes obtained from the double logarithmic plot suggest that the 100k1HA-1OVA conjugate adopts a random coil conformation like 100kHA.…”

“…We previously prepared various conjugates consisting of HA and bovine serum albumin (BSA) by changing the mixing ratio between the carboxylic groups of HA and the amino groups of BSA. 21 The resultant conjugates contained multiple HA and BSA molecules and showed almost the same size and morphology as HA, indicating that the conjugates adopted a highly condensed structure. The recombinant CD44 molecule recognized the conjugates more than it did HA before the conjugation.…”

“…17 Because HA possesses high biocompatibility and biodegradability, along with no toxicity, immunogenicity, or inflammatory effects, HA and its derivatives have been widely used as medical materials, such as for visco-supplementation, drug delivery, and tissue engineering. [18][19][20][21] HA can specifically bind to various HA receptors on the cell surface such as CD44 22,23 and receptors for HA-mediated motility (RHAMM). 24 Cells from a variety of cancers, such as epithelial, ovarian, colon, and stomach, overexpress these HA-binding receptors.…”