Interaction between Cervical Microbiota and Host Gene Regulation in Caesarean Section Scar Diverticulum

Yang, Xing; Pan, Xinyi; Li, Manchao; Zeng, Zhi; Guo, Yanxian; Chen, Panyu; Liang, Xiaoyan; Chen, Peigen; Liu, Guihua

doi:10.1128/spectrum.01676-22

Cited by 14 publications

(12 citation statements)

References 48 publications

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“…Metabolite detection was performed using liquid chromatography (LC) and mass spectrometry (MS) (Acquity UPLC I Class plus, Waters). The extraction and detection procedures of metabolites were described in previous studies ( 35 ). Briefly, after samples were thawed at room temperature, 1 mg was added to a 1.5-mL Eppendorf tube, and 20 μL of an ice-cold mixture of methanol and acetonitrile (2/1 [vol/vol]) was added.…”

Section: Methodsmentioning

confidence: 99%

Interaction between Host and Microbes in the Semen of Patients with Idiopathic Nonobstructive Azoospermia

Chen

Zhu

et al. 2023

Microbiol Spectr

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Section: Methodsmentioning

confidence: 99%

Interaction between Host and Microbes in the Semen of Patients with Idiopathic Nonobstructive Azoospermia

Chen

Zhu

et al. 2023

Microbiol Spectr

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“…Caesarean section scar diverticulum (CSD) significantly affects female fertility, posing a challenge to women who wish to conceive. Our research team conducted a preliminary investigation on bacteria-host interactions [12]. Current findings suggest that fungi play crucial roles in maintaining bacterial community and microbiome stability [32, 33].…”

Section: Discussionmentioning

confidence: 99%

“…We achieved more precise bacterial community compositions by implementing higher-resolution metagenomics than our previous study [12]. Lactobacillus jensenii of the Lactobacillus genus exhibited the greatest discrepancy between the two groups.…”

Section: Discussionmentioning

confidence: 99%

“…The sample collection procedure is as described above [12]. In short, after cleaning the external genitalia and vagina, a sterile disposable swab is inserted into the cervical canal, and the swab is rotated five times to collect the sample fully.…”

Section: Participant Recruitment and Sample Collectionmentioning

confidence: 99%

“…Our previous studies have demonstrated that the abnormal alterations in the local microbiota of Cesarean scar defects (CSD) cause continuous leakage through local inflammation and immune imbalance [11]. Further investigations of ours revealed the underlying mechanism demonstrating that abnormal bacteria in CSD deplete protective fatty acids and generate N-(3-hydroxy-eicosanoyl)-homoserine lactone, thus leading to promoting apoptosis of vascular endothelial cells and endometrial epithelial cells, ultimately impairing women’s reproductive capacity [12]. Nonetheless, the causative factors behind bacterial dysbiosis in complex microbial communities remain poorly understood.…”

Section: Introductionmentioning

confidence: 99%

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Fungal-bacteria interactions provide shelter for bacteria in Caesarean section scar diverticulum

Chen,

Liu

et al. 2023

Preprint

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Caesarean section scar diverticulum (CSD) is a significant cause of infertility among women who have previously had a Caesarean section, primarily due to persistent inflammatory exudation associated with this condition. Even though abnormal bacterial composition is identified as a critical factor leading to this chronic inflammation, clinical data suggests that a long-term cure is often unattainable with antibiotic treatment alone. In our study, we employed metagenomic analysis and mass spectrometry techniques to investigate the fungal composition in CSD and its interaction with bacteria. We discovered that local fungal abnormalities in CSD can disrupt the stability of the bacterial population and the entire microbial community by altering bacterial abundance via specific metabolites. For instance, Lachnellula suecica reduces the abundance of several Lactobacillus spp., such as Lactobacillus jensenii, by diminishing the production of metabolites like Goyaglycoside A and Janthitrem E. Concurrently, Clavispora lusitaniae and Ophiocordyceps australis can synergistically impact the abundance of Lactobacillus spp. by modulating metabolite abundance. Our findings underscore that abnormal fungal composition and activity are key drivers of local bacterial dysbiosis in CSD.

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Effect of hysteroscopic surgery on IVF/ICSI pregnancy outcomes for different cesarean scar diverticulum severity: A retrospective cohort study

Pan,

Xu,

Liu

et al. 2024

Intl J Gynecology & Obste

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ObjectiveTo investigate associations between hysteroscopic surgery for patients with varying cesarean scar diverticulum (CSD) severity and in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) embryo transfer (ET) pregnancy outcomes, focusing also on the correlation between the CSD size with its severity, and pregnancy outcomes.MethodsA retrospective study was conducted on patients with CSD who underwent IVF/ICSI‐ET at a university‐based hospital between January 2017 and July 2023. Patients were categorized into four groups based on CSD severity and whether they received hysteroscopic surgery: a mild surgical group (Group A, n = 86), a mild non‐surgical group (Group B, n = 30), a moderate‐to‐severe surgical group (Group C, n = 173), and a moderate‐to‐severe non‐surgical group (Group D, n = 96). Baseline characteristics and pregnancy outcomes were compared among these groups. Correlation assessments were conducted to explore relationships between CSD size with its severity, and pregnancy outcomes.ResultsCompared with Group D, Group C exhibited significantly increased rates of biochemical pregnancy (odds ratio [OR] 1.90; 95% confidence interval [CI] 1.03–3.51, P = 0.041), clinical pregnancy (OR 2.30; 95% CI1.18–4.45; P = 0.014), and live birth (OR 2.77; 95% CI 1.10–7.00, P = 0.031). However, no differences in pregnancy outcomes were observed between Groups A and B. Correlation analyses revealed significant positive associations between CSD severity and its depth, length, width, and volume.ConclusionsPatients with moderate‐to‐severe CSD achieved favorable IVF/ICSI pregnancy outcomes following hysteroscopic surgery. The CSD size was significantly related to its severity.

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Interaction between Cervical Microbiota and Host Gene Regulation in Caesarean Section Scar Diverticulum

Abstract: Cesarean section scar diverticulum (CSD) has become a formidable obstacle preventing women receiving CS from reproducing. In this study, we revealed that potentially harmful microbes do have adverse effects on the host endometrium.

Cited by 14 publications

References 48 publications

Interaction between Host and Microbes in the Semen of Patients with Idiopathic Nonobstructive Azoospermia

Interaction between Host and Microbes in the Semen of Patients with Idiopathic Nonobstructive Azoospermia

Fungal-bacteria interactions provide shelter for bacteria in Caesarean section scar diverticulum

Effect of hysteroscopic surgery on IVF/ICSI pregnancy outcomes for different cesarean scar diverticulum severity: A retrospective cohort study

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