Interaction between Covalent DNA Gels and a Cationic Surfactant

Costa, Diana; Hansson, Per; Schneider, Stefanie; Miguel, Maria G.; Lindman, Björn

doi:10.1021/bm0508919

Cited by 54 publications

(51 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The transition was produced by adding acetone to the solution: at about 60 vol.% acetone the DNA hydrogel suddenly collapses to a volume about 1/15 of that in the swollen state. Similar, but less marked transition is found upon increasing the concentration of divalent calcium cations [112,113], of spermidine, a trivalent cation [114], and of cationic surfactant [115,116]. This multivalent cation induced collapse is interpreted as a result of the association of DNA double strands that follows their charge neutralization [112].…”

Section: Hydrogelsmentioning

confidence: 74%

DNA-Based Soft Phases

Bellini¹,

Cerbino²,

Zanchetta³

2011

Topics in Current Chemistry

View full text Add to dashboard Cite

This chapter reviews the state-of-the-art in the study of molecular or colloidal systems whose mutual interactions are mediated by DNA molecules. In the last decade, the robust current knowledge of DNA interactions has enabled an impressive growth of self-assembled DNA-based structures that depend crucially on the properties of DNA-DNA interactions. In many cases, structures are built on design by exploiting the programmable selectivity of DNA interactions and the modularity of their strength. The study of DNA-based materials is definitely an emerging field in condensed matter physics, nanotechnology, and material science. This chapter will consider both systems that are entirely constructed by DNA and hybrid systems in which latex or metal colloidal particles are coated by DNA strands. We will confine our discussion to systems in which DNA-mediated interactions promote the formation of "phases," that is structures extending on length scales much larger than the building blocks. Their self-assembly typically involves a large number of interacting particles and often features hierarchical stages of structuring. Because of the possibility of fine-tuning the geometry and strength of the DNA-mediated interactions, these systems are characterized by a wide variety of patterns of self-assembly, ranging from amorphous, to liquid crystalline, to crystalline in one, two, or three dimensions.

show abstract

Section: Hydrogelsmentioning

confidence: 74%

DNA-Based Soft Phases

Bellini¹,

Cerbino²,

Zanchetta³

2011

Topics in Current Chemistry

View full text Add to dashboard Cite

show abstract

“…Techniques that have been used to determine the cac of DNA-surfactant systems include fluorescence spectroscopy, 16,17 potentiometric titrations, 18 and DNA gel swelling experiments. 19 Electric conductivity measurements provide more accurate measurements than potentiometric titrations and have a clear advantage over the fluorescence spectroscopy since it does not require the addition of fluorescent probes. Regarding the DNA gels, it is unclear whether the constraints suffered by the DNA inside the gel network affects the cac values.…”

Section: Introductionmentioning

confidence: 99%

Interaction between DNA and Cationic Surfactants: Effect of DNA Conformation and Surfactant Headgroup

et al. 2008

Self Cite

View full text Add to dashboard Cite

The interactions between DNA and a number of different cationic surfactants, differing in headgroup polarity, were investigated by electric conductivity measurements and fluorescence microscopy. It was observed that, the critical association concentration (cac), characterizing the onset of surfactant binding to DNA, does not vary significantly with the architecture of the headgroup. However, comparing with the critical micelle concentration (cmc) in the absence of DNA, it can be inferred that the micelles of a surfactant with a simple quaternary ammonium headgroup are much more stabilized by the presence of DNA than those of surfactants with hydroxylated headgroups. In line with previous studies of polymer-surfactant association, the cac does not vary significantly with either the DNA concentration or its chain length. On the other hand, a novel observation is that the cac is much lower when DNA is denaturated and in the single-stranded conformation, than for the double-helix DNA. This is contrary to expectation for a simple electrostatically driven association. Thus previous studies of polyelectrolytesurfactant systems have shown that the cac decreases strongly with increasing linear charge density of the polyion. Since double-stranded DNA (dsDNA) has twice as large linear charge density as single-stranded DNA (ssDNA), the stronger binding in the latter case indicates an important role of nonelectrostatic effects. Both a higher flexibility of ssDNA and a higher hydrophobicity due to the exposed bases are found to play a role, with the hydrophobic interaction argued to be more important. The significance of hydrophobic DNA-surfactant interaction is in line with other observations. The significance of nonelectrostatic effects is also indicated in significant differences in cac between different surfactants for ssDNA but not for dsDNA. For lower concentrations of DNA, the conductivity measurements presented an "anomalous" feature, i.e., a second inflection point for surfactant concentrations below the cac; this feature was not displayed at higher concentrations of DNA. The effect is attributed to the presence of a mixture of ss-and dsDNA molecules. Thus the stability of dsDNA is dependent on a certain ion atmosphere; at lower ion concentrations the electrostatic repulsions between the DNA strands become too strong compared to the attractive interactions, and there is a dissociation into the individual strands. Fluorescence microscopy studies, performed at much lower DNA concentrations, demonstrated a transformation of dsDNA from an extended "coil" state to a compact "globule" condition, with a broad concentration region of coexistence of coils and globules. The onset of DNA compaction coincides roughly with the cac values obtained from conductivity measurements. This is in line with the observed independence of cac on the DNA concentration, together with the assumption that the onset of binding corresponds to an initiation of DNA compaction. No major changes in either the onset of compaction or complete compaction...

show abstract

“…[1][2][3][4][5][6][7][8][9][10][11][12] In fact, the physicochemical properties of gels have often been explained in terms of the conformation of a subchain in network. However, an essential difference exists between a gel and a single polymer chain; subchains are fixed by a network structure, whereas a single polymer chain is not fixed.…”

Section: Introductionmentioning

confidence: 99%

Marked differences in volume phase transitions between gel and single molecule in DNA

Mayama

Nakai

Takushi

et al. 2007

The Journal of Chemical Physics

View full text Add to dashboard Cite

Volume phase transitions of a DNA gel and a single giant DNA chain caused by spermidine 3+ ͑SPD 3+ ͒ were investigated. The change in volume for the single DNA ͑V / V 0 ϳ 10 −5 ͒ was four orders of magnitude greater than that for the DNA gel ͑ϳ10 −1 ͒, while the critical SPD 3+ concentration for the gel ͑1.8 mM͒ was one order of magnitude greater than that of the single DNA ͑0.12-0.25 mM͒ at the same pH 6.86. We tried to describe mean-field theories with virial expansion, which is valid for the coil-globule transition of a single polymer chain, for the volume phase transitions to explain the reason why such marked differences appeared. Considering the degree of the ordering of Kuhn segments arising from the gel network structure together with the chain length of cross-linked polymer chains, the volume phase transitions were described and then the significant differences were reproduced quantitatively. We concluded that the network structure plays a significant role in the volume phase transition of the gel.

show abstract

Interaction between Covalent DNA Gels and a Cationic Surfactant

Cited by 54 publications

References 56 publications

DNA-Based Soft Phases

DNA-Based Soft Phases

Interaction between DNA and Cationic Surfactants: Effect of DNA Conformation and Surfactant Headgroup

Marked differences in volume phase transitions between gel and single molecule in DNA

Contact Info

Product

Resources

About