Interaction between denatured DNA and RNA from Bacillus stearothermophilus involving only one-half of total DNA

Interaction between Denatured DNA and Ribosomal RNA from Bacteria: Characterisation of DNA Sites Interacting with Ribosomal RNA at Room Temperature

“…As the extent of DNA-RNA interaction a t room temperature seems to be dependent on the source of DNA, but not of ribosomal RNA [8], the same…”

“…This kind of DNA-RNA interaction has been observed in DNA-RNA mixtures containing DNA from several bacilli but no formation of a pseudohybrid band had been observed when the mixture contained DNA:from Escherichiu coli [8,5].…”

“…After two doublings, a hundred fold excess of unlabeled uracil was added and, after 0.8 doublings, growth was stopped by pouring the culture on crushed ice. Cells were collected by centrifugation and RNA extracted as previously reported [8]. This RNA had a specific activity of about 5,000 counts/min/pg and contained two components having sedimentation coefficients of 16 and 23 S respectively.…”

“…I n previous works [8,5] it has been demonstrated that only one of the two complementary DNA strands is involved in this kind of interaction with ribosomal RNA, and work subsequently performed using DNA from phage a [9] has shown that only the strand which is physioEnzymes. Pancreatic ribonuclease, ribonucleate pyrimidine-nucleotido-2'-transferase (cyclizing) (EC 2.7.7.16); ribonuclease T 1, ribonucleate guaninenucleotide-2'-transferase (cyclizing) (EC 2.7.7.26); deoxyribonuclease, deoxyribonucleate oligonucleotidohydrolase (EC 3.1.4.5).logically transcribed participates to the formation of pseudohybrids.This kind of DNA-RNA interaction has been observed in DNA-RNA mixtures containing DNA from several bacilli but no formation of a pseudohybrid band had been observed when the mixture contained DNA:from Escherichiu coli [8,5].A similar kind of interaction has been shown to take place between denatured DNA and polyguanylic acid [5,6,10].I n phage 1, in which transcription occurs on alternating regions of both DNA strands [ll, 121 the sites interacting with poly G are located on the transcribed region of each strand [13,14].The interaction a t room temperature between denatured DNA and ribosomal RNA has now been investigated by means of the nitrocellulose membrane filter technique [15] and by gel atration. Moreover we have further investigated DNA-RNA mixtures containing DNA from E. coli and observed in this case too the existence of a DNA-RNA interaction a t room temperature.…”

“…I n previous works [8,5] it has been demonstrated that only one of the two complementary DNA strands is involved in this kind of interaction with ribosomal RNA, and work subsequently performed using DNA from phage a [9] has shown that only the strand which is physioEnzymes. Pancreatic ribonuclease, ribonucleate pyrimidine-nucleotido-2'-transferase (cyclizing) (EC 2.7.7.16); ribonuclease T 1, ribonucleate guaninenucleotide-2'-transferase (cyclizing) (EC 2.7.7.26); deoxyribonuclease, deoxyribonucleate oligonucleotidohydrolase (EC 3.1.4.5).…”