Interaction between handling induced stress and anxiolytic effects of ethanol in zebrafish: A behavioral and neurochemical analysis

Tran, Steven; Nowicki, Magda; Fulcher, Niveen; Chatterjee, Diptendu

doi:10.1016/j.bbr.2015.10.061

Cited by 39 publications

(14 citation statements)

References 41 publications

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“…This result suggests that the interaction of METH with social isolation, a stressful stimulus in highly social species like zebrafish, is the behind of the observed differences in the mobility between the locomotor and NTT assays. In rodents and zebrafish, stressors have been shown to potentiate the effects of drugs on locomotor activity ( Arias et al, 2010 ; Tran et al, 2016 ), but the effect of the interactions between neuroactive drugs and stressors on locomotor activity is generally difficult to interpret ( Tran and Gerlai, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

A Zebrafish Model of Neurotoxicity by Binge-Like Methamphetamine Exposure

et al. 2021

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Hyperthermia is a common confounding factor for assessing the neurotoxic effects of methamphetamine (METH) in mammalian models. The development of new models of methamphetamine neurotoxicity using vertebrate poikilothermic animals should allow to overcome this problem. The aim of the present study was to develop a zebrafish model of neurotoxicity by binge-like methamphetamine exposure. After an initial testing at 20 and 40 mg/L for 48 h, the later METH concentration was selected for developing the model and the effects on the brain monoaminergic profile, locomotor, anxiety-like and social behaviors as well as on the expression of key genes of the catecholaminergic system were determined. A concentration- and time-dependent decrease in the brain levels of dopamine (DA), norepinephrine (NE) and serotonin (5-HT) was found in METH-exposed fish. A significant hyperactivity was found during the first hour of exposure, followed 3 h after by a positive geotaxis and negative scototaxis in the novel tank and in the light/dark paradigm, respectively. Moreover, the behavioral phenotype in the treated fish was consistent with social isolation. At transcriptional level, th1 and slc18a2 (vmat2) exhibited a significant increase after 3 h of exposure, whereas the expression of gfap, a marker of astroglial response to neuronal injury, was strongly increased after 48 h exposure. However, no evidences of oxidative stress were found in the brain of the treated fish. Altogether, this study demonstrates the suitability of the adult zebrafish as a model of METH-induced neurotoxicity and provides more information about the biochemical and behavioral consequences of METH abuse.

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Section: Discussionmentioning

confidence: 99%

A Zebrafish Model of Neurotoxicity by Binge-Like Methamphetamine Exposure

et al. 2021

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“…The anxiolytic effects of citalopram are likely related to its direct influence on serotonergic concentrations. For example, handling stress has been shown to increase anxiety-like behavior and reduce brain concentrations of the serotonin metabolite 5-Hydroxyindoleacetic acid (5-HIAA) (Tran et al, 2016a). Furthermore, Maximino et al (2014) observed that acute administration of the 5-HT1a receptor agonist buspirone reduced behavioral anxiety in the light-dark test.…”

Section: Discussionmentioning

confidence: 99%

Comparison between two- and three-dimensional scoring of zebrafish response to psychoactive drugs: identifying when three-dimensional analysis is needed

Macrı̀

Clément

Spinello

et al. 2019

PeerJ

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Zebrafish (Danio rerio) have recently emerged as a valuable laboratory species in the field of behavioral pharmacology, where they afford rapid and precise high-throughput drug screening. Although the behavioral repertoire of this species manifests along three-dimensional (3D), most of the efforts in behavioral pharmacology rely on two-dimensional (2D) projections acquired from a single overhead or front camera. We recently showed that, compared to a 3D scoring approach, 2D analyses could lead to inaccurate claims regarding individual and social behavior of drug-free experimental subjects. Here, we examined whether this conclusion extended to the field of behavioral pharmacology by phenotyping adult zebrafish, acutely exposed to citalopram (30, 50, and 100 mg/L) or ethanol (0.25%, 0.50%, and 1.00%), in the novel tank diving test over a 6-min experimental session. We observed that both compounds modulated the time course of general locomotion and anxiety-related profiles, the latter being represented by specific behaviors (erratic movements and freezing) and avoidance of anxiety-eliciting areas of the test tank (top half and distance from the side walls). We observed that 2D projections of 3D trajectories (ground truth data) may introduce a source of unwanted variation in zebrafish behavioral phenotyping. Predictably, both 2D views underestimate absolute levels of general locomotion. Additionally, while data obtained from a camera positioned on top of the experimental tank are similar to those obtained from a 3D reconstruction, 2D front view data yield false negative findings.

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“…The anxiolytic effects of citalopram are likely related to its direct influence on serotonergic concentrations. For example, handling stress has been shown to increase anxietylike behavior and reduce brain concentrations of the serotonin metabolite 5-HIAA (Tran et al 2016b). Furthermore, (Maximino et al 2014) observed that acute administration of the 5-HT1a receptor agonist buspirone reduced behavioral anxiety in the light-dark test.…”

Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "Comparison between two- and three-dimensional scoring of zebrafish response to psychoactive drugs: identifying when three-dimensional analysis is needed (v0.1)"

2019

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Zebrafish (Danio rerio) have recently emerged as a valuable laboratory species in the field of behavioral pharmacology, where they afford rapid and precise high-throughput drug screening. Although the behavioral repertoire of this species manifests along three dimensions (3D), most of the efforts in behavioral pharmacology rely on 2D projections acquired from a single overhead or front camera. We recently showed that, compared to a 3D scoring approach, 2D analyses could lead to inaccurate claims regarding individual and social behavior of drug-free experimental subjects. Here, we examined whether this conclusion extended to the field of behavioral pharmacology by phenotyping adult zebrafish, acutely exposed to citalopram (30 mg/L, 50 mg/L, and 100 mg/L) or ethanol (0.25%, 0.50%, and 1.00%), in the novel tank diving test over a six-minute experimental session. We observed that both compounds modulated the time course of general locomotion and anxiety-related profiles, the latter being represented by specific behaviors (erratic movements and freezing) and avoidance of anxiety-eliciting areas of the test tank (top half and distance from the side walls). We observed that 2D projections of 3D trajectories (ground truth data) may introduce a source of unwanted variation in zebrafish behavioral phenotyping. Predictably, both 2D views underestimate absolute levels of general locomotion. Additionally, while data obtained from a camera positioned on top of the experimental tank are similar to those obtained from a 3D reconstruction, 2D front view data yield false negative findings.

show abstract

Interaction between handling induced stress and anxiolytic effects of ethanol in zebrafish: A behavioral and neurochemical analysis

Cited by 39 publications

References 41 publications

A Zebrafish Model of Neurotoxicity by Binge-Like Methamphetamine Exposure

A Zebrafish Model of Neurotoxicity by Binge-Like Methamphetamine Exposure

Comparison between two- and three-dimensional scoring of zebrafish response to psychoactive drugs: identifying when three-dimensional analysis is needed

Peer Review #1 of "Comparison between two- and three-dimensional scoring of zebrafish response to psychoactive drugs: identifying when three-dimensional analysis is needed (v0.1)"

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