Interaction between Human Serum Albumin and Different Anatase TiO₂ Nanoparticles: A Nano-bio Interface Study

Abstract

In this investigation, differently shaped and surface functionalized TiO 2 anatase nanoparticles and human serum albumin (HSA) were selected to study proteinnanoparticles interaction both in a solution and on flat surfaces, thereby mimicking a medical device. Anatase nanocrystals were characterized by transmission electron microscopy (TEM), Brunauer-Emmett-Teller (BET) surface analysis and dynamic light scattering (DLS). The proteinnanoparticles' interactions and their eventual reversibility were studied by pH dependent ζ-potential measurements in different media: ultra-pure water, a phosphate buffer simulating physiological conditions and in a culture medium supplemented with foetal bovine serum. The protein corona masking effect was evidenced and the interaction HSA-nanocrystals resulted irreversible. The interaction HSA-silicon supported TiO 2 nanocrystals films was studied by atomic force microscopy (AFM), and resulted driven by the substrate hydrophilicity degree plus was different for the diverse range of nanocrystals tested. Surface roughness measurements showed that on some of the nanocrystals, HSA were arranged in a more globular manner. A lower protein affinity was found for nanocrystals that had a smaller primary particle size, which may correspond to their higher biocompatibility. This nano-bio interface research aimed to study the HSA protein-TiO 2 anatase nanocrystals under conditions similar to those for in vitro and in vivo toxicity analyses.

Immobilization of Titanium(IV) Oxide onto 3D Spongin Scaffolds of Marine Sponge Origin According to Extreme Biomimetics Principles for Removal of C.I. Basic Blue 9

“…Based on the obtained data and literature reports regarding TiO 2 –protein interactions [59,60,61,62,63], we proposed a plausible mechanism of titanium dioxide bonding to the surface of spongin (see Figure 7). Multiple functional groups are present at the surface of spongin and might be binding positions involved in the bonding of titanium dioxide.…”

A comparative study of simulated body fluids in the presence of proteins

“…major criticism of the method is the lack of organics, especially proteins, which are abundant in human blood plasma (roughly 70 g/L in human blood plasma [17]) and are well-known to have specific/nonspecific adsorption on various surfaces, which could affect the nucleation & growth of apatite in SBF [18] [19] [20] [21] [22] [23]. In fact, proteins, along with other organic molecules, are also active players in the regulation of the biomineralization processes in vivo [24].…”

A Facile Approach to Fabricate Dual Purpose Hybrid Materials for Tissue Engineering and Water Remediation

“…On the other hand, TiO 2 nanoparticles can participate in electrostatic interaction with amino groups of collagen. It is known that the anatase phase of TiO 2 nanoparticles can interact with biomolecules electrostatically 37,38 . When both OSS and TiO 2 nanoparticles are used to synthesize the hybrid scaffolds, the combined interactions induce enhanced thermal and other stabilities to the collagen matrix in comparison to the treatment with OSS alone.…”