Interaction between immobilized polyelectrolyte complex nanoparticles and human mesenchymal stromal cells

Woltmann, Beatrice; Torger, Bernhard; Müller, Martin; Hempel, Ute

doi:10.2147/ijn.s61198

Cited by 6 publications

(3 citation statements)

References 46 publications

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“…Of course, the drug delivery system must be biocompatible. The cytocompatibility of PECNP in human MSC has been proven in previous studies [ 20 , 32 ]. Only osteoclasts showed a reduced activity and delayed differentiation after addition of high concentrations of PECNP [ 17 ].…”

Section: Discussionmentioning

confidence: 89%

Effects of BDNF and PEC Nanoparticles on Osteocytes

et al. 2020

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Bone substitute materials loaded with mediators that stimulate fracture healing are demanded in the clinical treatment in trauma surgery and orthopedics. Brain-derived neurotrophic factor (BDNF) enhances the proliferation and differentiation of mesenchymal stem cells into osteoblast. To load the implants with BDNF, a drug delivery system that allows the release of BDNF under spatiotemporal control would improve functionality. Polyelectrolyte complex nanoparticles (PECNP) have been reported as a suitable drug delivery system. The suitability of PECNP in contact with osteocytes as the main cell type of bone is not known so far. Thus, we aimed to verify that BDNF and PECNP loaded with BDNF (PECNP+BDNF) as well as pure PECNP have no negative effects on osteocytes in vitro. Therefore, the murine osteocyte cell line MLO-Y4 was treated with BDNF and PECNP+BDNF. The effects on proliferation were analyzed by the BrdU test (n = 5). The results demonstrated a significant increase in proliferation 24 h after BDNF application, whereas PECNP+BDNF did not lead to significant changes. Thus, we conclude that BDNF is an appropriate mediator to stimulate osteocytes. Since the addition of PECNP did not affect the viability of osteocytes, we conclude that PECNP are a suitable drug delivery system for bone implants.

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Section: Discussionmentioning

confidence: 89%

Effects of BDNF and PEC Nanoparticles on Osteocytes

et al. 2020

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“…The Hofmeister series was discovered in protein precipitation experiments, , and this series classifies anions and cations according to their increasing precipitation power as follows: SCN − < ClO 4 − < normalI − < NO 3 − < Br − < Cl − < normalF − < normalH 2 PO 4 − Li + < Na + < normalK + < Rb + < Cs + < NH 4 + < false( CH 3 false) 4 normalN + This list of ions rank-ordered in terms of how strongly they modulate protein solubility provides a path for the modification of PEC properties simply by changing the type of salt during their preparation. Clearly, PECs are versatile materials that could possibly be used in a wide range of applications, such as matrices for enzyme immobilization, biocompatible coatings for drug delivery, and vectors for gene therapy, , to name a few.…”

Section: Introductionmentioning

confidence: 99%

“…This list of ions rank-ordered in terms of how strongly they modulate protein solubility provides a path for the modification of PEC properties simply by changing the type of salt during their preparation. Clearly, PECs are versatile materials that could possibly be used in a wide range of applications, such as matrices for enzyme immobilization, 19 biocompatible coatings for drug delivery, 20 and vectors for gene therapy, 21,22 to name a few. The process of interpolyelectrolyte neutralization is commonly studied by light scattering methods, turbidimetry, conductometry, potentiometry, spectrophotometry, and calorimetry.…”

Section: Introductionmentioning

confidence: 99%

High-Resolution Ultrasonic Spectroscopy: Looking at the Interpolyelectrolyte Neutralization from a Different Perspective

et al. 2023

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In this study, the high-resolution ultrasonic spectroscopy (HR-US) technique was applied to examine interpolyelectrolyte neutralization. The mentioned method was tested on the example of complexation between poly(allylammonium) cations and poly(acrylate) anions in aqueous solutions at pH = 7. It was confirmed by HR-US that the type of titration (stepwise or abrupt), the direction of titration, and the type of background salt affect the outcome of interpolyelectrolyte neutralization. The obtained results were explained on the basis of ultrasonic velocity and attenuation changes in the context of suspension compressibility, a parameter that is extremely sensitive to molecular organization and intermolecular interactions. Moreover, the results of HR-US measurements proved to be consistent with previous results obtained by more traditional methods such as dynamic light scattering, microcalorimetry, and electrokinetics. This research demonstrates that HR-US is a convenient and reliable method that can be employed for the investigation of interpolyelectrolyte neutralization and polyelectrolyte-related processes.

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Biorelated Polyelectrolyte Coatings Studied by in Situ Attenuated Total Reflection–Fourier Transform Infrared Spectroscopy: Deposition Concepts, Wet Adhesiveness, and Biomedical Applications

2018

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In this conceptual contribution, thin functional coatings consisting of either pure polyelectrolytes (PELs) or complexes between oppositely charged PELs at model and applied substrates are outlined. Latter PEL/PEL complexes were deposited by two concepts. In a first well-known concept, PEL multilayers (PEM) were consecutively deposited according to the layer-by-layer (LbL) technique. In a second less known concept, PEL complex (PEC) nanoparticles (NPs) preformed by mixing polycation (PC) and polyanion (PA) solutions were deposited in one step. Both concepts based on binary oppositely charged PELs are compared to one based on a single polycation system. Examples shall be given on adhesiveness, nanostructure, and biomedical applications of PEM and PEC NP coatings. In situ attenuated total reflection (ATR) infrared (IR) spectroscopy, circular dichroism (CD), and scanning force microscopy (SFM) were used for molecular, optical, and microscopic characterization. At first, results on the adsorbed amount and wet adhesiveness of pure (single-component) PEL coatings as a function of charge density are given to motivate coatings of mixed oppositely charged PELs. Second, the wet adhesiveness of PEM and PEC NP coatings of identical PEL compounds in aqueous media varying the molar charge ratio ( n-/ n+) and the deposition step z, respectively, is compared. Upon comparing the three PEL deposition concepts, it is suggested that the lack or absence of excess charge at the PEL/surface interface is one of the main factors for the wet adhesiveness of all pure PEL, PEM, and PEC NP coatings. Finally, the potential of PEM and PEC NP coatings for biomedical applications is outlined. Concerning biopassivation, PEM coatings excessed or terminated by PA repel proteins with low isoelectric points. Concerning bioactivation, PEM coatings loaded with antibiotics as well as PEC NP coatings loaded with therapeutic bisphosphonates showed retarded, optionally temperature responsive drug release for applications in acute surgery and bone healing, and immunoglobulin/PEL complex coatings might open theranostic applications.

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Interaction between immobilized polyelectrolyte complex nanoparticles and human mesenchymal stromal cells

Cited by 6 publications

References 46 publications

Effects of BDNF and PEC Nanoparticles on Osteocytes

Effects of BDNF and PEC Nanoparticles on Osteocytes

High-Resolution Ultrasonic Spectroscopy: Looking at the Interpolyelectrolyte Neutralization from a Different Perspective

Biorelated Polyelectrolyte Coatings Studied by in Situ Attenuated Total Reflection–Fourier Transform Infrared Spectroscopy: Deposition Concepts, Wet Adhesiveness, and Biomedical Applications

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