Interaction Between Ketoconazole and Human Serum Albumin on Epoxy Modified Magnetic Nanoparticles for Drug Delivery

Abstract

Superparamagnetic iron oxide nanoparticles (SPIONs) were modified with [3-(2,3-epoxy-propoxy)propyl] trimethoxy silane, which resulted in formation of epoxy groups on the particles surface. The epoxy functionalized SPIONs can bind to human serum albumin (HSA). The binding amount of HSA to epoxy modified SPIONs was found to be as 32.7 microM for 1 mg epoxy modified SPIONs at 280 and 342 nm of excitation and emission wavelengths by using fluorescence spectroscopy. Interaction of ketoconazole with HSA immobilized epoxy functionalized SPIONs was studied at 300 and 390 nm of excitation and emission wavelengths, respectively. Binding mechanism of ketoconazole and HSA was identified by Stern-Volmer equation. Thermodynamic parameters (deltaH, deltaS and deltaG) were also estimated for the interaction. Therefore, the nature of the binding forces was found to be as hydrophobic interaction. Protein and drug attachments were also examined by Infrared spectroscopy (IR) and Scanning Electron Microscopy (SEM). This study show that prepared albumin-based magnetic nanoparticles carrier systems represents an attractive strategy for drug delivery.

Spectrofluorometric and thermal gravimetric study on binding interaction of thiabendazole with hemoglobin on epoxy-functionalized magnetic nanoparticles

“…1b). The peaks at 2913 cm −1 and 2854 cm −1 come from C\ \H stretching vibration [17,18]. The presence of the anchored epoxy silane group is confirmed by the stretching vibration of Si\ \O that appears at 1080 cm −1 [19].…”

“…We have identified interactions of several drugs with proteins on functionalized Fe 3 O 4 nanoparticles in previous studies [18,21]. In this study, GPTS modified Fe 3 O 4 magnetic nanoparticles were synthesized for hemoglobin binding as shown in Scheme 1.…”

Spectroscopic investigation of water‐soluble alloyed QDs with bovine serum albumin

“…As such there is an enhanced interest in the studies on interaction between nanomaterials and biomolecules such as proteins (1)(2)(3)(4)(5)(6)(7). As such there is an enhanced interest in the studies on interaction between nanomaterials and biomolecules such as proteins (1)(2)(3)(4)(5)(6)(7).…”

“…Nanotechnology, an extremely powerful emerging technology, is expected to have a substantial impact on the field of pharmaceutical and medical diagnostics. As such there is an enhanced interest in the studies on interaction between nanomaterials and biomolecules such as proteins (1)(2)(3)(4)(5)(6)(7). Nanomaterials can influence the structural and functional properties of proteins and as such there is increasing interest to understand the effects of various nanomaterials on their interaction with biomolecules.…”

Surface Modification of Superparamagnetic Magnetite Nanoparticles and Its Application for Detection of Anti-CEA Using Electrochemiluminescent Immunosensor

“…In comparison with the other methods, such as radioimmunoassay [21], fluorescence immunoassay [22], chemiluminescence immunoassay [23], and enzyme-linked immunosorbent assay (ELISA) [24], the electrochemiluminescence immunoassay (ECLIA) method has considerable advantages, including lack of radioactive pollution, high sensitivity, good selectivity, wide linear range, good stability, fast inspection speed, high automation, and wide application range [25]. In recent years, with the development of nanoscience and nanotechnology, nanoparticles have been applied as the labels in different field [26][27][28][29][30]. Magnetic nanoparticles have revealed unique physical, chemical, thermal, and magnetic properties that made them attractive in many applications [31][32][33][34].…”