Interaction between myoglobin and hyaluronic acid in layer-by-layer structures—An electrochemical study

Methylene blue and neutral red electropolymerisation on AuQCM and on modified AuQCM electrodes: an electrochemical and gravimetric study

“…At this potential the unmodified AuQCM electrode also has a small value of R ct , due to the proximity to the hydrogen evolution potential. At À0.48 V vs. SCE, both PNR-modified electrodes present the same charge transfer resistance of 5.8 kO cm 2 .…”

“…1 Other modified AuQCM electrodes, which showed viability for use in biosensors, were recently developed: hyaluronic acid and myoglobin layer-by-layer modified AuQCM electrodes. 2,3 This type of protein immobilisation allows the intercalation of very small amounts of compound in an ultrathin nano-ordered structure, without alteration of its initial conformation.…”

Microwave-assisted synthesis of hemin–graphene/poly(3,4-ethylenedioxythiophene) nanocomposite for a biomimetic hydrogen peroxide biosensor

“…4 As an articial enzyme mimetic, 5 hemin has numerous advantages compared with traditional enzymes and precious metal catalysts, which are either extremely costly or easily inuenced by environmental temperature, pH value and toxic chemicals. 6 However, to date, too many efforts have concentrated on exploring how to enhance the direct electrochemistry of heme proteins such as myoglobin 7,8 and hemoglobin [9][10][11][12] with deeply buried redoxactive centers inside the proteins, ignoring the role of hemin itself, the actual active center of the heme-proteins. If we can obtain new hemin-based composites, an excellent level of direct electrochemistry may be conveniently achieved.…”

Mechanism of Formation and Construction of Self-Assembled Myoglobin/Hyaluronic Acid Multilayer Films: An Electrochemical QCM, Impedance, and AFM Study

“…A previous paper described the cyclic voltammetric behavior of the {HA/Mb} n films during the film formation and also the stability of the films in time. The electrochemical characteristics of the {HA/Mb} n modified electrode were compared with the response of the electrode in solution containing the Mb or of an electrode modified with a thin layer of Mb, in order to identify the electroactive species and elucidate the electron-transfer mechanism . There were indications of the release of electroactive heme contained by the protein in such LBL structures.…”

“…HA is a polymer of disaccharides, themselves composed of d -glucuronic acid and d - N -acetylglucosamine, linked via alternating β-1,4 and β-1,3 glycosidic bonds. At pH 5.0 (p K a = 2.9), the carboxyl groups of the glucuronic acids are deprotonated so that the polymer will be negatively charged …”