Interaction between PAMAM-NH2 G4 dendrimer and 5-fluorouracil in aqueous solution

Buczkowski, Adam; Sękowski, Szymon; Grala, Aleksandra; Palecz, Danuta; Miłowska, Katarzyna; Urbaniak, Paweł; Gabryelak, Teresa; Piekarski, Henryk; Pałecz, Bartłomiej

doi:10.1016/j.ijpharm.2011.02.014

Cited by 56 publications

(38 citation statements)

References 45 publications

(53 reference statements)

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“…However, the encapsulation of fluorouracil (5-FU) by PAMAM showed different results. The maximum combined 5-FU was 90 which indicated that the drug molecules are combined not only with the surface amine groups but also with those inside (27). Thus, the binding sites on PAMAM were likely the surface amine groups through hydrogen bonds, electrostatic and/or hydrophobic interactions (12).…”

Section: H Nmr Spectroscopymentioning

confidence: 99%

“…7b). The coefficients of the line could be ascribed to a physical conception (27). The directional coefficient (12.82) was interpreted as the maximum amount of AS combined with one PAMAM molecule.…”

Section: H Nmr Spectroscopymentioning

confidence: 99%

“…A single molecule of PAMAM G4 contains 250 potential bonding sites, which includes 64 surface primary amino groups, 62 internal tertiary amine groups, and 124 amide groups (27). The maximum amount of combined AS was far less than the total potential binding sites or any part of binding sites of PAMAM.…”

Section: H Nmr Spectroscopymentioning

confidence: 99%

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Encapsulation of Acetylshikonin by Polyamidoamine Dendrimers for Preparing Prominent Nanoparticles

Peng¹,

Zhou²,

Xia³

et al. 2014

AAPS PharmSciTech

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Abstract. Acetylshikonin (AS) has demonstrated antitumor potential. However, the development of therapeutic applications utilizing AS is inhibited by its poor solubility in water. In the present work, polyamidoamine (PAMAM) dendrimers and their PEGylated derivatives were employed to increase the solubility of AS. A distinct color transition was observed during the encapsulation of AS suggesting strong intermolecular forces between PAMAM and AS. Ultraviolet-visible, high-performance liquid chromatography, and 1 H NMR were used to verify the interaction between PAMAM and AS. The maximum amount of combined AS to each PAMAM molecule was determined. The cytotoxicity of AS nanoparticles was evaluated against leukemia (K562) and breast cancer (SK-BR-3) cell lines; the AS nanoparticles were shown to effectively inhibit tumor cells.

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Section: H Nmr Spectroscopymentioning

confidence: 99%

Section: H Nmr Spectroscopymentioning

confidence: 99%

See 1 more Smart Citation

Encapsulation of Acetylshikonin by Polyamidoamine Dendrimers for Preparing Prominent Nanoparticles

Peng¹,

Zhou²,

Xia³

et al. 2014

AAPS PharmSciTech

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“…The concentration of 5-fluorouracil in the solutions of PAMAM G5-NH 2 and PAMAM G5-OH dendrimer was determined by the spectrophotometric method (Specord50 Analytic Jena), as in our previous works (Buczkowski et al, 2011(Buczkowski et al, , 2012a. There was prepared 1 ml of each dendrimer under investigation with a concentration of 2.5 mM-50 mM.…”

Section: Measurements Of Solubilitymentioning

confidence: 99%

“…To date our team has investigated the processes of attachment of various ligands of biological and pharmacological importance Buczkowski et al, 2013, including 5-fluorouracil (Buczkowski et al, 2011(Buczkowski et al, , 2012a, to the macromolecules of PAMAM G4-NH 2 dendrimer. The aim of this study is to study the interactions of two commercially available dendrimers: PAMAM G5-NH 2 and PAMAM G5-OH with 5-fluorouracil molecules, to assess the effect of the terminal group character and increase in steric hindrance in the macromolecule structure on the saturation of active sites with oncological drug.…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic and calorimetric studies of formation of the supramolecular complexes of PAMAM G5-NH2 and G5-OH dendrimers with 5-fluorouracil in aqueous solution

Buczkowski

Olesiński

Zbicinska

et al. 2015

International Journal of Pharmaceutics

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Spectroscopic and molecular modeling studies of the interaction between morin and polyamidoamine dendrimer

2013

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Interactions between the polyamidoamine (PAMAM) dendrimer and drug molecules are of interest for their potential biomedical applications. The goal of this work is to examine the interaction of PAMAM-C12 25% dendrimer with morin. The ultraviolet-visible, fluorescence spectroscopic methods as well as molecular modeling were used to analyze drug-binding mode, binding constants and binding sites, etc. The experimental data showed that the binding constant of morin-PAMAM-C12 25% is about 10(5) L/mol. The interaction of morin with PAMAM-C12 25% is mainly driven by the hydrophobic, electrostatic, hydrogen bonds and van der Waals forces. There are mainly three classes of binding site of morin at the interface of PAMAM-C12 25%. These results provided some useful information for self-assembling and disassembling the PAMAM dendrimer as well as efficient drug delivery and therapeutic applications.

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Interaction between PAMAM-NH2 G4 dendrimer and 5-fluorouracil in aqueous solution

Cited by 56 publications

References 45 publications

Encapsulation of Acetylshikonin by Polyamidoamine Dendrimers for Preparing Prominent Nanoparticles

Encapsulation of Acetylshikonin by Polyamidoamine Dendrimers for Preparing Prominent Nanoparticles

Spectroscopic and calorimetric studies of formation of the supramolecular complexes of PAMAM G5-NH2 and G5-OH dendrimers with 5-fluorouracil in aqueous solution

Spectroscopic and molecular modeling studies of the interaction between morin and polyamidoamine dendrimer

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