Interaction between Polyamines and Bacterial Outer Membranes as Investigated with Ion-Selective Electrodes

Katsu, Takashi; Nakagawa, Hideki; Yasuda, Keiko

doi:10.1128/aac.46.4.1073-1079.2002

Cited by 49 publications

(51 citation statements)

References 27 publications

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“…Although required, the cationic charge is not sufficient per se to cause either the permeabilisation of bacterial membranes or the bacteria killing (Katsu et al, 2002;Savage et al, 2002). In fact, the hydrophobic moiety of CAAs is responsible for their insertion into the hydrophobic core of the bacterial lipid bilayer, leading to membrane permeabilisation and eventually to CAAs-mediated bacterial killing as convincingly indicated by the lack of membrane permeabilisation of a deacylated polymyxin analogue (Vaara and Vaara, 1983;Tsubery et al, 2000Tsubery et al, , 2001Tsubery et al, , 2002Katz et al, 2003;Clausell et al, 2005Clausell et al, , 2007.…”

Section: Introductionmentioning

confidence: 97%

“…Whereas the interaction of other CAAs with biological membranes has been the subject of numerous studies, particularly with polymyxin B (PMB) (Chen and Feingold, 1972;Clausell et al, 2004Clausell et al, , 2005Clausell et al, , 2007Hartmann et al, 1978;Katsu et al, 1984Katsu et al, , 2002Katz et al, 2003;Galla, 1981, 1982;Tsubery et al, 2000Tsubery et al, , 2001Tsubery et al, , 2002Wiese et al, 1998Wiese et al, , 2003, relatively little information is available regarding the interaction of SQ with cellular membranes (Chen et al, 2006;Salmi et al, 2008a;Selinsky et al, 1998Selinsky et al, , 2000.…”

Section: Introductionmentioning

confidence: 99%

“…CAAs include cationic surfactants, cyclic lipopeptides, ␤-sheet and ␣-helix-forming peptides and cholesterol derivatives (Savage et al, 2002). The antibacterial activity of CAAs relies on their ability to interact with bacterial lipids, especially lipopolysaccharide (LPS) and phosphatidylglycerol (PG), resulting in: (i) the release of divalent cations bridging LPS molecules together, (ii) a change in the outer membrane permeability barrier, (iii) the release of membrane vesicle, (iv) membrane lipid exchange, which in turn causes membrane permeabilisation and bacteria killing (Katsu et al, 1984(Katsu et al, , 2002Savage et al, 2002;Clausell et al, 2004Clausell et al, , 2005Clausell et al, , 2007McBroom and Kuehn, 2007). Binding of CAAs to bacterial membranes involves both electrostatic and hydrophobic interactions (Savage et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biophysical studies of the interaction of squalamine and other cationic amphiphilic molecules with bacterial and eukaryotic membranes: importance of the distribution coefficient in membrane selectivity

Pasquale

Salmi-Smail

Brunel

et al. 2010

Chemistry and Physics of Lipids

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biophysical studies of the interaction of squalamine and other cationic amphiphilic molecules with bacterial and eukaryotic membranes: importance of the distribution coefficient in membrane selectivity

Pasquale

Salmi-Smail

Brunel

et al. 2010

Chemistry and Physics of Lipids

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“…Katsu et al 63 reported that the outer membrane of gramnegative bacteria prevents the penetration of hydrophobic antibiotics into cells. Hence, the cationic compounds are used to enhance the permeability of the outer membrane and thereby increase the sensitivity of gram-negative bacteria to several antibiotics.…”

Section: Resultsmentioning

confidence: 99%

Tailored Silica Nanoparticles Surface to Increase Drug Load and Enhance Bactericidal Response

Oliveira

Bouchmella

Picco

et al. 2017

J. Braz. Chem. Soc.

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Nanoparticles' surface properties can be used as triggers to regulate or even enhance biological response and generate tailored structures to substitute conventional antibiotics. Here, silica nanoparticles surface was duly tuned in order to increase the water-insoluble drug load (curcumin) and improve the antibacterial activity. Our main motivation was based on the electrostatic attraction between the positively charged amino groups and the negatively charged curcumin and/or bacteria membrane. In addition, the variation of amino grafting amount on silica nanoparticles indicated that the grafting increase was directly related to the extent of drug entrapped into the nanoparticles as well as to the bactericidal activity. The combination of amino-functionalized silica nanoparticles associated with the presence of curcumin allowed to produce a dual bactericidal system that shows promising perspective for its use in biomedical applications.Keywords: amino-functionalized silica nanoparticles, curcumin, bactericidal activity, Escherichia coli IntroductionPathogenic microorganisms have proved to develop resistance to currently available antibiotics becoming a worldwide concern.1-5 Thus, it is necessary to find new systems or drugs presenting alternative mechanisms of action to substitute the existing commercial antibiotics.Nanoparticles are promising materials for therapeutic applications since they possess unique physical and chemical properties. [6][7][8][9] Thus, silica-based nanoparticles have been widely investigated and used in different fields, including materials science and biomedicine, due to their stability, high hydrophilic surface, biocompatibility and easy surface functionalization. [10][11][12][13] In particular, mesoporous silica nanoparticles are potential candidates as drug carriers since they provide the possibility of encapsulating and delivering large quantities of drugs due to large surface areas and pore volumes. [14][15][16][17][18][19][20] Using the well-known silane chemistry, silica nanoparticles have been functionalized with a variety of different functional groups such as carboxyl, 21 vinyl, 22 amino, 23 mercapto 24 and epoxy. 25 The surface functionalization with organic functional groups allows increasing the storage capacity of drugs or biomolecules within the silica pores. 21,[25][26][27][28] Moreover, the chemical surface modification of the nanoparticles is an important tool to control the interaction of nanoparticles with biological systems while reducing toxicity and increasing the therapeutic effects. [29][30][31][32] Typical surface modification methods via covalent bonds are (i) the co-condensation (one-pot synthesis method); (ii) the postsynthetic grafting (PSG) and (iii) the use of bissilylated organic precursors that generate periodic mesoporous organosilicas (PMOs). [33][34][35] The PSG involves modification of silica after the synthesis. In this method, it is possible to restrict the functionalization of surface-accessible silanol groups both within the mesopore network and o...

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“…The TPP ϩ electrode was constructed as reported previously. 25) The E. coli cells harboring recombinant plasmids were grown in Tanaka medium supplemented with 1% tryptone, 20 mM potassium lactate and 10 mg/ml of chloramphenicol at 37°C until OD 650 of 0.5. The cells were harvested and washed 3 times with 0.1 M MOPS-KOH, pH 7.0.…”

Section: Tppmentioning

confidence: 99%

Gene Cloning and Characterization of EfmA, a Multidrug Efflux Pump, from Enterococcus faecium

Nishioka

Ogawa

Kuroda

et al. 2009

Biological & Pharmaceutical Bulletin

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A DNA fragment responsible for resistance to antimicrobial agents was cloned from chromosomal DNA of Enterococcus faecium FN-1, a clinically isolated strain. Escherichia coli KAM32, a drug-hypersusceptible mutant, was used as a host for gene cloning. Cells of E. coli KAM32 harboring a recombinant plasmid (pTFM8) carrying the DNA fragment became resistant to fluoroquinolones, macrolides, ethidium bromide, 4,6-diamidino-2-phenylindole (DAPI) and tetraphenylphosphonium chloride (TPPCl). Three complete open reading frames (ORFs) were found in the DNA insert of pTFM8, and the deduced amino acid sequences of one of the ORFs showed high similarity to Mdt(A) from Lactococcus lactis. Mdt(A) is a multidrug efflux pump belonging to a major facilitator superfamily. We designated the ORF efmA. E. coli KAM32 cells harboring the efmA showed energy-dependent efflux of DAPI and TPP ؉ . We also observed norfloxacin/H ؉ antiport due to EfmA. The mRNA expression of efmA was observed in E. faecium FN-1 grown without any exogenously added antimicrobial agents. Thus, we conclude that efmA is constitutively expressed under laboratory growth conditions and would contribute to intrinsic resistance against multiple antimicrobial agents in E. faecium FN-1.

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Interaction between Polyamines and Bacterial Outer Membranes as Investigated with Ion-Selective Electrodes

Cited by 49 publications

References 27 publications

Biophysical studies of the interaction of squalamine and other cationic amphiphilic molecules with bacterial and eukaryotic membranes: importance of the distribution coefficient in membrane selectivity

Biophysical studies of the interaction of squalamine and other cationic amphiphilic molecules with bacterial and eukaryotic membranes: importance of the distribution coefficient in membrane selectivity

Tailored Silica Nanoparticles Surface to Increase Drug Load and Enhance Bactericidal Response

Gene Cloning and Characterization of EfmA, a Multidrug Efflux Pump, from Enterococcus faecium

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