Interaction between Room Temperature Ionic Liquid [bmim]BF₄ and DNA Investigated by Electrochemical Micromethod

Abstract

Ionic liquids (ILs) as a kind of novel green solvent are being widely used in various researches related to the life sciences and chemistry, which demands the knowledge of interaction between ILs and biomacromolecules. However, the almost completely inert optical, electric, thermal properties of ILs make it difficult to directly obtain information about the interactions. Herein, by using a hydrophilic ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim]BF4) as a model, the electrostatic interaction between ILs and calf thymus DNA (ctDNA) was investigated by a surface electrochemical micromethod. A convenient and simple method was established to obtain the thermodynamic and kinetic information about the DNA-IL interaction only with microscale sample consumption. The quantitative thermodynamic and kinetic parameters about the interaction of [bmim]BF4 and ctDNA, such as the binding constant (K), the Gibbs energy of surface binding (DeltaGb), and the dissociation rate constant (k), were obtained for the first time.

Electrochemical Biosensor Based on Carbon Ionic Liquid Electrode Modified with Nafion/Hemoglobin/DNA Composite Film

“…As indicated in [24], on the surface of CILE a layer of cationic ion was present. Also the interaction of DNA with imidazolium type ionic liquid had been reported by resonance light scattering spectroscopy [31,32] and the electrochemical micromethod [33]. After applied DNA on the surface of CILE, the cationic 1-ethyl-3-methylimidazolium (EMIM þ ) from the ionic liquid can attack the P-O bonds of the phosphate groups in DNA fragments by electrostatic attraction.…”

Exploring the thermal stability of DNA-linked gold nanoparticles in ionic liquids and molecular solvents

“…15 Various spectroscopic and theoretic studies have also been performed to understand the interaction between IL and DNA. [16][17][18][19] In the past two decades, the function and application of DNA have expanded significantly. In addition to its genetic role, DNA has been used in nanostructure design, [20][21][22] catalysis, 23 biosensor development, [24][25][26][27] and materials science.…”

Application of ionic liquid–dsDNA biocomposite film for the direct electrochemistry of myglobin on carbon ionic liquid electrode

“…According to the references [20,23,24], IL can interact with dsDNA and entrap into the three-dimensional dsDNA network by intercalation to give a flexible composite with high ionic conductivity. Xie et al also investigated the interaction between BMIMBF 4 and dsDNA with electrochemical micromethod in detail [25]. The resulting IL-robed DNA adduct showed specific characteristics and had the potential application as a new kind of biomaterial.…”