Interaction between two <i>cis</i>‐acting elements, ABRE and DRE, in ABA‐dependent expression of <i>Arabidopsis rd29A</i> gene in response to dehydration and high‐salinity stresses

Narusaka, Yoshihiro; Nakashima, Kenichiro; Shinwari, Zabta Khan; Sakuma, Yoh; Furihata, Takashi; Abe, Hiroshi; Narusaka, Mari; Shinozaki, Kazuo; Yamaguchi-Shinozaki, Kazuko

doi:10.1046/j.1365-313x.2003.01708.x

Cited by 663 publications

(448 citation statements)

References 43 publications

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“…Contrary to our results, Narusaka et al (2003) showed that artificial reporter constructs in which all cis elements apart from the DRE (CRT) elements were eliminated, fail to be activated by ABA treatment, indicating that this element cannot, in isolation, respond to ABA (Narusaka et al, 2003). However, this lack of response to ABA is consistent with data from the same laboratory that indicated CBF1-4 are not ABAresponsive.…”

Section: Discussionsupporting

confidence: 62%

Abscisic Acid Induces CBF Gene Transcription and Subsequent Induction of Cold-Regulated Genes via the CRT Promoter Element

et al. 2004

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Many cold-regulated genes of Arabidopsis are inducible by abscisic acid (ABA) as well as by cold. This has been thought to occur via two separate signaling pathways, with ABA acting via ABA-responsive promoter elements and low temperature activating the C-repeat element (CRT; dehydration-responsive) promoter element via CBF (DREB1) transcription factors. We show here that ABA is also capable of activating the CRT promoter element. Although the more recently discovered ABAinducible CBF4 transcription factor might have accounted for this, we show here that CBF1-3 transcript levels also increase in response to elevated ABA levels. This increase in CBF1-3 transcript levels appears to be at least in part due to increased activity of the CBF promoters in response to ABA. A total of 125 bp of the CBF2 promoter, which has previously been shown to be sufficient for cold-, mechanical-, and cycloheximide-induced expression, was also sufficient for ABA-induced expression. However, the ABA-responsive promoter element-like motif within this region is not needed for ABA-induced expression. An observed increase in CBF protein levels after ABA treatment, together with previous data showing that increased CBF levels are sufficient for cold-regulated gene induction, suggests that ABA-induced increases in CBF1-3 transcript levels do have the potential to activate the CRT. Our data indicate therefore that activation of the CRT may also occur via a novel ABA-inducible signaling pathway using the normally cold-inducible CBFs.The phytohormone abscisic acid (ABA) is involved in mediating responses to a number of environmental stresses including drought (Leung and Giraudat, 1998). Cellular ABA accumulates in response to such stresses, and increases in its concentration can lead to a number of physiological adaptations, including stomatal closure and growth inhibition, as well as up-regulation of specific genes. Many of the genes that are inducible by ABA are also expressed in response to cold and/or drought conditions; for example, the Arabidopsis cold-regulated (COR) genes RAB18, LTI78, and KIN2 (Kurkela and Franck, 1990; Lång and Palva, 1992;Nordin et al., 1993;Mäntylä et al., 1995). Several signaling pathways leading to COR gene expression have been described, including both ABA-dependent and ABA-independent pathways (Shinozaki and Yamaguchi-Shinozaki, 2000). The gene LTI78 (also known as COR78 or RD29A) has been studied as a paradigm system for a gene whose expression can be controlled by osmotic stress, low temperature, and ABA (Yamaguchi-Shinozaki and Shinozaki, 1994; Ishitani et al., 1997; Liu et al., 1998) via either ABAdependent or ABA-independent routes .Two cis-acting elements have been identified in the promoter of LTI78 and other COR genes that control expression under different stress conditions. The dehydration-responsive (DRE) element (YamaguchiShinozaki and Shinozaki, 1994) has been shown to mediate both cold-and osmotic stress-inducible ABA-independent gene expression (Liu et al., 1998;Shinozaki and Yamaguchi-Shinozak...

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Section: Discussionsupporting

confidence: 62%

Abscisic Acid Induces CBF Gene Transcription and Subsequent Induction of Cold-Regulated Genes via the CRT Promoter Element

et al. 2004

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“…A5 and BS, Expressing the TaCHP transgene; C13, negative control (empty vector only). AtRD29A promoter, and the two pathways coactivate the expression of AtRD29A (Narusaka et al, 2003). In Figure 9, we present the model we have elaborated to describe the function of TaCHP.…”

Section: Tachp Is Involved In An Aba-dependent Pathwaymentioning

confidence: 99%

“…The latter is mediated mainly through an IP3-Ca 2+ -DREB cascade (Zhu, 2001), while the former involves AREB and other modules. Both pathways require participation of many regulatory factors (Chinnusamy et al, 2004), and there is a degree of cross talk between the two pathways (Haake et al, 2002;Narusaka et al, 2003). However, the detailed characteristic of the cross talk is still not well dissected.…”

mentioning

confidence: 99%

TaCHP: A Wheat Zinc Finger Protein Gene Down-Regulated by Abscisic Acid and Salinity Stress Plays a Positive Role in Stress Tolerance

Zhao

et al. 2010

Plant Physiology

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The plant response to abiotic stresses involves both abscisic acid (ABA)-dependent and ABA-independent signaling pathways. Here we describe TaCHP, a CHP-rich (for cysteine, histidine, and proline rich) zinc finger protein family gene extracted from bread wheat (Triticum aestivum), is differentially expressed during abiotic stress between the salinity-sensitive cultivar Jinan 177 and its tolerant somatic hybrid introgression cultivar Shanrong No.3. TaCHP expressed in the roots of seedlings at the three-leaf stage, and the transcript localized within the cells of the root tip cortex and meristem. TaCHP transcript abundance was higher in Shanrong No.3 than in Jinan 177, but was reduced by the imposition of salinity or drought stress, as well as by the exogenous supply of ABA. When JN17, a salinity hypersensitive wheat cultivar, was engineered to overexpress TaCHP, its performance in the face of salinity stress was improved, and the ectopic expression of TaCHP in Arabidopsis (Arabidopsis thaliana) also improved the ability of salt tolerance. The expression level of a number of stress reporter genes (AtCBF3, AtDREB2A, AtABI2, and AtABI1) was raised in the transgenic lines in the presence of salinity stress, while that of AtMYB15, AtABA2, and AtAAO3 was reduced in its absence. The presence in the upstream region of the TaCHP open reading frame of the cis-elements ABRE, MYBRS, and MYCRS suggests that it is a component of the ABA-dependent and -independent signaling pathways involved in the plant response to abiotic stress. We suggest that TaCHP enhances stress tolerance via the promotion of CBF3 and DREB2A expression.

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“…MYB, CBF/DREB1, HSF, TGA6, BOS1, bZIP, AP2/EREBP etc. are indicated for their role in plant development and stress tolerance (Meshi and Iwabuchi 1995;Xiong et al 1997;Kizis et al 2001;Narasuka et al 2003;Mengiste et al 2003).…”

Section: Introductionmentioning

confidence: 99%

MYB transcription factor genes as regulators for plant responses: an overview

Ambawat

Sharma

Yadav

et al. 2013

Physiol Mol Biol Plants

838

544

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Regulation of gene expression at the level of transcription controls many crucial biological processes. Transcription factors (TFs) play a great role in controlling cellular processes and MYB TF family is large and involved in controlling various processes like responses to biotic and abiotic stresses, development, differentiation, metabolism, defense etc. Here, we review MYB TFs with particular emphasis on their role in controlling different biological processes. This will provide valuable insights in understanding regulatory networks and associated functions to develop strategies for crop improvement.

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Interaction between two cis‐acting elements, ABRE and DRE, in ABA‐dependent expression of Arabidopsis rd29A gene in response to dehydration and high‐salinity stresses

Cited by 663 publications

References 43 publications

Abscisic Acid Induces CBF Gene Transcription and Subsequent Induction of Cold-Regulated Genes via the CRT Promoter Element

Abscisic Acid Induces CBF Gene Transcription and Subsequent Induction of Cold-Regulated Genes via the CRT Promoter Element

TaCHP: A Wheat Zinc Finger Protein Gene Down-Regulated by Abscisic Acid and Salinity Stress Plays a Positive Role in Stress Tolerance

MYB transcription factor genes as regulators for plant responses: an overview

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