We report a new electron interaction effect in GaAs/AlGaAs quantum wires. Using DC-bias spectroscopy, we show that large and abrupt changes occur to the energies of spin-down (lower energy) states as they populate. The effect is not observed for spin-up energy states. At B=0, interactions have a pronounced effect, in the form of the well-known 0.7 Structure. However, our new results show that interactions strongly affect the energy spectrum at all magnetic fields, from 0 to 16 T, not just in the vicinity of the 0.7 Structure.PACS numbers: 72.25.Dc, 73.21.Hb, 73.23.Ad Semiconductor nanostructures such as quantum wires are involved in the development of future quantum technologies, so it is important that their electronic properties are understood. Ballistic quantum wires are ideal quantum laboratories for studying many-body physics, due to the low electron densities combined with the low levels of disorder which can now be achieved. However, despite their simple geometry, the properties of these quasione-dimensional (Q1D) systems are much less well understood than those of 2D or 0D systems. An example of this is the 0.7 Structure [1], a spin-related phenomenon which has attracted much interest in recent years, although its exact origin is still the subject of debate. It has recently been discovered that the 0.7 Structure is not an isolated phenomenon, but has a high-magnetic field variant which occurs at crossings of Zeeman-split 1D subbands [2].In this paper, we report a new interaction effect which demonstrates that electron-electron interactions profoundly affect the electronic structure of the quantum wire at all magnetic fields, not just in the region of the 0.7 Structure or its high-field version, the Analog [2]. Using DC-bias spectroscopy [3], we show that as spin-down (lower energy) subbands begin to populate, they abruptly drop in energy by as much as 0.5 meV, within a vanishingly small range of gate-voltage. These results also cast doubt on basic assumptions which are often made when analysing quantum-wires -such as the idea that electron density is proportional or equivalent to gate-voltage, and the validity of an 'exchange-enhanced g-factor' in quantum wires [4].Our new findings also provide an explanation for one of the most characteristic features of the 0.7 Structure, which has, to our knowledge, not been explained by any model or theory so far -the question of how the 0.7 Structure can survive to such high temperatures, when all other conductance features have disappeared [4].Our samples consist of split-gate devices [5] defined by electron-beam lithography on a Hall bar etched from a GaAs/Al x Ga 1−x As heterostructure. All the samples used in this work have a length of 0.4 µm and a width of 0.7 µm. The two-dimensional electron gas (2DEG) formed 292 nm below the surface has a mobility of 1.1 × 10 6 cm 2 /Vs and a carrier density of 1.15 × 10 11 cm −2 . In the parallel magnetic field regime, using the Hall voltage, the out-of-plane misalignment was measured to be 0.3 • . The measurement temper...