Interaction Effects of Temperature, Light, Nutrients, and pH on Growth and Competition of Chlorella vulgaris and Anabaena sp. Strain PCC

Meng, Shun Long; Chen, Xi; Wang, Jing; Fan, Liang Jen; Qiu, Li Ping; Zheng, Yao; Chen, Jia Zhang; Xu, Pao

doi:10.3389/fenvs.2021.690191

Cited by 14 publications

(2 citation statements)

References 51 publications

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“…A total of 600 mg gold particles with 3.3 mg plasmid DNA and 250 mg gold particles with 20 mg plasmid DNA were employed by different groups (Fukuda et al, 2008;Son et al, 2012). Furthermore, interactions among different parameters can affect transformation efficiency (Meng et al, 2021). Previously, only single factors have been analyzed while not considering the interactions among factors.…”

Section: Discussionmentioning

confidence: 99%

A Toolbox for Constructing a Stable Genetic Transformation Platform Allowing Foreign Fragment Integration in the Genome of Neopyropia yezoensis

et al. 2022

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Neopyropia yezoensis, previously known as Pyropia yezoensis, is regarded as a model macroalgae because of its economic value and typical intertidal seaweed species. However, the genetic transformation system to introduce foreign genes into its cells is inefficient and not stable. This study developed a more efficient transformation toolbox that allows the stable expression of foreign genes in N. yezoensis cells. We constructed an efficient transformation platform with the parameters of biolistic bombardment of 6 cm target distance, 600 μg gold particles/shot, 10 μg plasmid DNA/shot, 1,350 psi of helium, and 30 mmHg vacuum pressure. Thalli at 35 days of age were the most suitable transformation conditions, in which the highest transformation efficiency was generated. The endogenous promoter pPyACT1 could control gene expression efficiently compared to pPyUBC, pPyDPE2, and pPyEF1-a, especially the exogenous promoter d35S. Finally, the foreign genes PyGUS and PyHygR were stably expressed in different generations of transformants, including monospores, gametophytes, and filamentous sporophytes. Southern blotting analysis confirmed that PyGUS was integrated into the genome of N. yezoensis transformants. Establishing an efficient gene expression toolbox provides a strong foundation for functional genomics research and molecular genetic breeding on N. yezoensis.

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Section: Discussionmentioning

confidence: 99%

A Toolbox for Constructing a Stable Genetic Transformation Platform Allowing Foreign Fragment Integration in the Genome of Neopyropia yezoensis

et al. 2022

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“…The growth rate of algae is only significantly limited by the N/P ratio at low N and P concentrations, and the optimal N/P ratio required for the growth of algae varies depending on the species of algae. For example, the competitive inhibition parameter (α) of Microcystis aeruginosa against Oscillatoria was greater than that (β) of Oscillatoria against Microcystis aeruginosa at medium and high N/P ratios, whereas the opposite phenomenon was observed at low N/P ratios [42]. Japanese scholar Seung HoBaek proposed the luxury consumption theory that excessive cell storage is not related to the growth rate [43].…”

Section: Long-term Nutrient Limitation Of Long-term Water Bodies In D...mentioning

confidence: 99%

Long-Term Variations of Biogenic Elements and Nutritional Status in Daya Bay, Northern South China Sea

Guo

Xiao

Liu

et al. 2023

JMSE

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This study explored the variations in the characteristics of the trophic structure of Daya Bay island waters over the last four decades based on the survey findings and research data on biogenic elements (dissolved inorganic nitrogen (DIN), NO2−, NO3−, NH4+, PO43−, and SiO32−) in Daya Bay during 1985–2021. At this time, the DIN concentration increased from 21.14 µg·L−1 to 558.42 µg·L−1 (26.41-fold increase), whereas the SiO32− concentration increased by only 3.6-fold. The PO43− concentrations attained a peak in 2004 and experienced a steady decline over the rest of the survey period. The fractions of NH4+, NO3−, and NO2− in DIN changed from 0.45, 0.40, and 0.15 in 1986 to 0.26, 0.74, and 0.003 in 2021, respectively. Overall, the mean values of NH4+, NO3−, and NO2− accounted for 45.2%, 42.5%, and 12.3%, respectively. The N/P(DIN/PO43−) ratio in Daya Bay increased from 28.08 in the 1980s to 51.63 in the 2010s. Meanwhile, the nutrient limitation conditions showed a gradual shift from N-limited to P-limited conditions. According to the nutrient quality index (NQI) analysis, the trophic state level of Daya Bay waters fell into the oligotrophic category 30 years ago (1985–2002, NQI < 2), whereas it increased from the mesotrophic level in 2005 (NQI = 2.03) to the eutrophic level in 2019 (NQI = 3.33) over the last 20 years. The results based on the eutrophication index (EI) of Daya Bay waters were generally consistent with those based on the NQI, displaying that the trophic level of Daya Bay waters indicated an increasing trend from 2005 to 2019. Moreover, the assessment data in 2021 indicated a decrease in the NQI to 0.90, thereby attaining the oligotrophic level again. This may be related to the decrease in aquacultural area in the bay over the last two years. The correlation analysis among the DIN, PO43−, and nutrient levels of Daya Bay waters indicated that the input of nitrogen and phosphorus was the primary reason for the higher nutrient levels in the water bodies; among them, municipal sewage discharge, aquaculture, and atmospheric deposition from industry are the main factors for the over importation. This indicates that the changes in the biogenic element concentrations led to variations in the trophic structure and level of Daya Bay and may be attributed to population growth and the development of the seaside industry and agriculture in the region.

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Nitrogen fixation rate and phosphorus enrichment effects on diazotrophic cyanobacteria in the Gulf of Riga

Liepina-Leimane,

Barda,

Jurgensone

et al. 2024

Mar Biol

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In eutrophied marine systems such as the Baltic Sea, diazotrophic cyanobacteria have the potential to add additional bioavailable nitrogen (N) to the system through fixation of atmospheric dinitrogen (N2). However, their growth is regarded to be limited by phosphorus availability (P). This study investigates the response of two cyanobacteria species, Aphanizomenon flosaquae and Nodularia spumigena, collected from the Gulf of Riga under different environmental conditions to a short-period dissolved inorganic phosphorus (DIP) enrichment. The samples were collected during the summer cyanobacterial bloom of 2022 in the central region of the Gulf of Riga. Contrary to expectations, neither species demonstrated a significant increase in biomass. The study also established that N2-fixation rates did not correlate directly with the total diazotrophic cyanobacteria biomass, but showed a significant correlation with heterocyst presence in both species addressed during this study. The findings suggest the influence of additional factors beyond DIP availability on the N2-fixing cyanobacteria growth in the Gulf of Riga.

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Interaction Effects of Temperature, Light, Nutrients, and pH on Growth and Competition of Chlorella vulgaris and Anabaena sp. Strain PCC

Cited by 14 publications

References 51 publications

A Toolbox for Constructing a Stable Genetic Transformation Platform Allowing Foreign Fragment Integration in the Genome of Neopyropia yezoensis

A Toolbox for Constructing a Stable Genetic Transformation Platform Allowing Foreign Fragment Integration in the Genome of Neopyropia yezoensis

Long-Term Variations of Biogenic Elements and Nutritional Status in Daya Bay, Northern South China Sea

Nitrogen fixation rate and phosphorus enrichment effects on diazotrophic cyanobacteria in the Gulf of Riga

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