Interaction mechanism between α-glucosidase and A-type trimer procyanidin revealed by integrated spectroscopic analysis techniques

Zhao, Li; Wen, Luming; Lu, Qun; Li, Rui

doi:10.1016/j.ijbiomac.2019.12.021

Cited by 37 publications

(27 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As presented in Figure 2 C , the 1/υ versus 1/[S] plots reveal a set of lines with various slopes and intercepts. The intersected point of lines in the second quadrant suggests that T 2 is a mixed‐type inhibitor [31] . The inhibition constants of T 1 binding with the free enzyme ( K i ) and the enzyme‐substrate complex ( K is ) were 7.7 μM and 31.46 μM, respectively ( Figure 2 D ).…”

Section: Resultsmentioning

confidence: 95%

See 1 more Smart Citation

Natural Triterpenoids Isolated from Akebia trifoliata Stem Explants Exert a Hypoglycemic Effect via α‐Glucosidase Inhibition and Glucose Uptake Stimulation in Insulin‐Resistant HepG2 Cells

Bian

Yang

Elango

et al. 2021

Chemistry & Biodiversity

View full text Add to dashboard Cite

The inhibition of α‐glucosidase activity is a prospective approach to attenuate postprandial hyperglycemia in the treatment of type 2 diabetes mellitus (T2DM). Herein, the inhibition of α‐glucosidase by three compounds T1–T3 of Akebia trifoliata stem, namely hederagenin (T1), 3‐epiakebonoic acid (T2), and arjunolic acid (T3) were investigated using enzyme kinetics and molecular docking analysis. The three triterpenoids exhibited excellent inhibitory activities against α‐glucosidase. T1–T3 showed the strongest inhibition with IC50 values of 42.1±5.4, 19.6±3.2, and 11.2±2.3 μM, respectively, compared to the acarbose positive control (IC50=106.3±8.2). Enzyme inhibition kinetics showed that triterpenoids T1–T3 demonstrated competitive, mixed, and noncompetitive‐type inhibition against α‐glucosidase, respectively. The inhibition constant (Ki) values were 21.21, 7.70, and 3.18 μM, respectively. Docking analysis determined that the interaction of ligands T1–T3 and α‐glucosidase was mainly forced by hydrogen bonds and hydrophobic interactions, which could result in improved binding to the active site of the target enzyme. The insulin resistant (IR)‐HepG2 cell model used in this study (HepG2 cells exposed to 10−7 M insulin for 24 h) and glucose uptake assays showed that compounds T1–T3 had no cytotoxicity with concentrations ranging from 6.25 to 25 μM and displayed significant stimulation of glucose uptake in IR‐HepG2 cells. Thus, triterpenoids T1–T3 showed dual therapeutic effects of α‐glucosidase inhibition and glucose uptake stimulation and could be used as potential medicinal resources to investigate new antidiabetic agents for the prevention or treatment of diabetes.

show abstract

Section: Resultsmentioning

confidence: 95%

“…Inhibitory kinetic mechanisms of α‐glucosidase by T 1 – T 3 were determined using Lineweaver–Burk plots, with methods similar to those previously reported [31,47] . Inhibition kinetics were performed by varying concentrations of T 1 – T 3 and detecting different concentrations of the inhibitor substrate.…”

Section: Methodsmentioning

confidence: 99%

Natural Triterpenoids Isolated from Akebia trifoliata Stem Explants Exert a Hypoglycemic Effect via α‐Glucosidase Inhibition and Glucose Uptake Stimulation in Insulin‐Resistant HepG2 Cells

Bian

Yang

Elango

et al. 2021

Chemistry & Biodiversity

View full text Add to dashboard Cite

show abstract

“…The chromatographic profile of a trichloroacetic acid (TCA) E. characias latex extract showed the presence of several carboxylic acids (aromatic and aliphatic) and alcoholic compounds [30]: benzoic acid (56), cinnamic acid (57), 4-hydroxybenzylalcohol (58), tyrosol (59), vanillic acid (60), p-coumaric acid (61), ferulic acid (62), sinapic acid (63), caffeic acid (64), 2-hydroxypropanoic acid (65), 2,3-dihydroxypropanoic acid (66), 3-hydroxypropanoic acid (67), 2-hydroxy-3-methylbutanoic acid (68), 4-hydroxybutanoic acid (69), 2-hydroxyesanoic acid (70), 3-phenylpropenoic acid (71) and 3-hydroxy-3-phenylpropenoic acid (72). Furthermore, some flavonoids were identified in latex but especially in aerial parts of the plant.…”

Section: Phenolic and Carboxylic Acid Compoundsmentioning

confidence: 99%

Euphorbia characias: Phytochemistry and Biological Activities

Fais

Delogu

Floris

et al. 2021

Plants

View full text Add to dashboard Cite

The aim of this review is to summarize all the compounds identified and characterized from Euphorbia characias, along with the biological activities reported for this plant. Euphorbia is one of the greatest genera in the spurge family of Euphorbiaceae and includes different kinds of plants characterized by the presence of milky latex. Among them, the species Euphorbia characias L. is an evergreen perennial shrub widely distributed in Mediterranean countries. E. characias latex and extracts from different parts of the plant have been extensively studied, leading to the identification of several chemical components such as terpenoids, sterol hydrocarbons, saturated and unsaturated fatty acids, cerebrosides and phenolic and carboxylic acids. The biological properties range between antioxidant activities, antimicrobial, antiviral and pesticidal activities, wound-healing properties, anti-aging and hypoglycemic properties and inhibitory activities toward target enzymes related to different diseases, such as cholinesterases and xanthine oxidase. The information available in this review allows us to consider the plant E. characias as a potential source of compounds for biomedical research.

show abstract

“…Procyanidins, a kind of flavonoids formed by the combination of catechin and epicatechin, was widely found in the plant such as vegetables, fruits, grains, and seeds (Rauf et al, 2019). Procyanidins were beneficial to humans for their antioxidant ability, metabolic regulation, obesity prevention, and hepatoprotective potential (Lobo et al, 2020;Wu et al, 2020;Zhao et al, 2020). Consequently, procyanidins can be used as a natural antioxidant in meat and meat product (Priyadarshi et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Microencapsulated procyanidins by extruding starch improved physicochemical properties, inhibited the protein and lipid oxidant of chicken sausages

Song

Jiang

et al. 2022

Journal of Food Science

View full text Add to dashboard Cite

Microencapsulated procyanidins by extruding starch (MPS) were used in meat and meat products as an antioxidant for their simple production process and high stability. This study investigated the controlled released properties of MPS and their effect on antioxidant capacity, physicochemical properties, and sensory qualities of chicken sausages during 4 • C storage within 28 days. Antioxidant capacity, particle size analysis, and simulated digestion in vitro demonstrated that microencapsulation by extruding starch delayed the procyanidins release. The reduced crystal structure of MPS was determined by the morphology observation (SEM) and the decrease of the typical diffraction peak at 2θ of 20.9 • (XRD). The MPS-added sausage had a higher (p < 0.05) ABTS and DPPH radical scavenging ratio (97.6% and 67.3%) and sulfhydryl contents (114.69 nmol/g protein) than other groups. Moreover, lower (p < 0.05) thiobarbituric acid reactive substances (TBARS) (0.67 mg MDA/kg sausage) and carbonyl values (3.24 nmol/mg protein) were detected in MPS-added sausages than others at the end of storage. The MPS addition increased redness (a* value) and decreased the lightness (L* value). The sensory analysis suggested that the sausage with the increased redness was favorable. These results denominated that MPS was an alternative antioxidant in chicken sausages.Practical Application: In this study, microencapsulated procyanidins were prepared by extrusion technology, and the effect on the quality of chicken sausages was investigated, which provides an alternative natural antioxidant for meat and meat products.

show abstract

Interaction mechanism between α-glucosidase and A-type trimer procyanidin revealed by integrated spectroscopic analysis techniques

Cited by 37 publications

References 47 publications

Natural Triterpenoids Isolated from Akebia trifoliata Stem Explants Exert a Hypoglycemic Effect via α‐Glucosidase Inhibition and Glucose Uptake Stimulation in Insulin‐Resistant HepG2 Cells

Natural Triterpenoids Isolated from Akebia trifoliata Stem Explants Exert a Hypoglycemic Effect via α‐Glucosidase Inhibition and Glucose Uptake Stimulation in Insulin‐Resistant HepG2 Cells

Euphorbia characias: Phytochemistry and Biological Activities

Microencapsulated procyanidins by extruding starch improved physicochemical properties, inhibited the protein and lipid oxidant of chicken sausages

Contact Info

Product

Resources

About