Interaction mechanism of aloe-emodin with trypsin: molecular structure–affinity relationship and effect on biological activities

Ren, Guoyan; Sun, He; Li, Gen; Fan, Jie; Du, Lin; Cui, Guoting

doi:10.1039/d0ra02712j

Cited by 7 publications

(1 citation statement)

References 40 publications

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“…They are so abundant that over 700 serine endopeptidases have been identified, accounting for a third of known peptidases [15]. They perform a variety of physiological functions such as digestion, immune response, blood clotting, fibrinolysis and reproduction [16]. For aquatic products, endogenous serine proteases may be present in the muscle tissue or digestive tract, such as myofibril-bound serine endopeptidase, sarcoplasmic serine proteinase, trypsin, etc.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of the Intestine Extracts and Their Effect on the Crude Collagen Fibers of the Body Wall from Sea Cucumber Apostichopus japonicus

Zhang

Nie

et al. 2023

Biology

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Sea cucumbers Apostichopus japonicus will vomit their intestines during certain stimulations, and the collagen of the body wall will then be degraded. To define the effect of the sea cucumber intestine extracts on the body wall, the intestinal extracts and crude collagen fibers (CCF) of sea cucumber A. japonicus were prepared. According to the gelatin zymography, the type of endogenous enzymes in intestinal extracts were mainly serine endopeptidases with optimal activities at pH 9.0 and 40 °C. According to the rheology results, the viscosity of 3% CCF decreased from 32.7 Pa·s to 5.3 Pa·s by adding intestine extracts. The serine protease inhibitor phenylmethanesulfonyl fluoride inhibited the activity of intestinal extracts and increased the viscosity of collagen fibers to 25.7 Pa·s. The results proved that serine protease in the intestinal extracts participated in the process of body wall softening in sea cucumbers.

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Section: Introductionmentioning

confidence: 99%

Characteristics of the Intestine Extracts and Their Effect on the Crude Collagen Fibers of the Body Wall from Sea Cucumber Apostichopus japonicus

Zhang

Nie

et al. 2023

Biology

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Effects of combined binding of chlorogenic acid/caffeic acid and gallic acid to trypsin on their synergistic antioxidant activity, enzyme activity and stability

Ren

et al. 2023

Food Chemistry: X

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Competitive binding of synergistic antioxidant chlorogenic acid and (−)-epigallocatechin gallate with lysozyme: Insights from multispectroscopic characterization, molecular docking and activity evaluation

Liu

Wang

et al. 2021

Journal of Molecular Liquids

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Interaction mechanism of aloe-emodin with trypsin: molecular structure–affinity relationship and effect on biological activities

Abstract: The mechanism of interaction between AE and trypsin was studied firstly. The biological activity of both decreased after the interaction. These results provide a basis for the development and utilization of AE.

Cited by 7 publications

References 40 publications

Characteristics of the Intestine Extracts and Their Effect on the Crude Collagen Fibers of the Body Wall from Sea Cucumber Apostichopus japonicus

Characteristics of the Intestine Extracts and Their Effect on the Crude Collagen Fibers of the Body Wall from Sea Cucumber Apostichopus japonicus

Effects of combined binding of chlorogenic acid/caffeic acid and gallic acid to trypsin on their synergistic antioxidant activity, enzyme activity and stability

Competitive binding of synergistic antioxidant chlorogenic acid and (−)-epigallocatechin gallate with lysozyme: Insights from multispectroscopic characterization, molecular docking and activity evaluation

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