Interaction mechanism of flavonoids with whey protein isolate: A spectrofluorometric and theoretical investigation

Li, Jiaqi; Tian, Ruijun; Liang, Guizhao; Shi, Ruijie; Hu, Jialun; Jiang, Zhanmei

doi:10.1016/j.foodchem.2021.129617

Cited by 38 publications

(12 citation statements)

References 31 publications

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“…In this process, the maximum peak of fluorescence spectrum changed slightly, indicating that the microenvironment around tyrosine residue and tryptophan residue changed. Similar results had previously been reported that whey protein changed its molecular conformation through interaction with puerarin, rutin and phloridin (Li et al, 2021).…”

Section: Synchronous Fluorescence Analysissupporting

confidence: 90%

Interaction mechanism of icariin and whey protein based on spectrofluorimetry and molecular docking

LI¹,

GE²

2023

Food Sci. Technol

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Icariin has low bioavailability and poor stability, which limits its wide application. The complexation of icariin and whey protein is expected to solve this problem, but there is no research on their interaction mechanism. In view of this, the related mechanism was studied systematically by spectrofluorimetry and molecular docking method in this study. The fluorescence analysis showed that icariin and whey protein could form a non-covalent complex driven by hydrophobic force, which led to the fluorescence quenching of whey protein. In this process, the microenvironment around the tyrosine residue and tryptophan residue of whey protein changed. The molecular docking analysis confirmed the existence of hydrophobic interaction and hydrogen bonding in the complex, which well confirmed fluorescence results. The obtained results can promote the application of icariin in food.

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Section: Synchronous Fluorescence Analysissupporting

confidence: 90%

Interaction mechanism of icariin and whey protein based on spectrofluorimetry and molecular docking

LI¹,

GE²

2023

Food Sci. Technol

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“…3D uorescence is oen utilized to investigate the structural changes in proteins bound to small molecules (HYP), and it shows the changes in protein Trp and Tyr residues. 41 Fig. 2(C and D) reveals that with the expansion of the HYP concentrations, the uorescence intensities of Peak 1 and 2 decreased.…”

Section: Synchronous and 3d Uorescence Spectrummentioning

confidence: 94%

Exploring the binding effects and inhibiting mechanism of hyperoside to lipase using multi-spectroscopic approaches, isothermal titration calorimetry, inhibition kinetics and molecular dynamics

Zeng

Tang

et al. 2023

RSC Adv.

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“…For static quenching, the binding constant (Ka) and binding site number (n) were computed using the double-logarithm equation [23]:…”

Section: Fluorescence Experimentsmentioning

confidence: 99%

Epicatechin attenuates lead (Pb)-induced cognitive impairment in mice: regulation on Nrf2 signaling pathway, and interference on the interaction between Pb with albumin

Cheng¹,

Zhu²,

Bu³

et al. 2024

Food Science and Human Wellness

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Epicatechin (EC) was used in this study to antagonize the cognitive dysfunction caused by lead (Pb) exposure in mice. Eight-week-old male Kunming mice were treated with PbCl 2 (20 mg/kg) and/or EC (50 mg/kg) by gavage administration for 4 weeks. Morris water maze test showed that EC could improve memory dysfunction induced by Pb. EC antagonized Ca 2+ overload, activated Nrf2 signaling pathway and reduced the accumulation of Pb in the brain and serum, which suggested that EC might alter Pb distribution in mice. In vitro, spectroscopic analysis, potentiometric titration and docking studies were applied to inquiry into the interaction between bovine serum albumin (BSA) and Pb 2+ in presence or absence of EC. EC was proved to chelate Pb 2+ and reduced the interaction between BSA and Pb 2+ . In summary, EC might protect Pb-induced cognitive impairment by activating Nrf2 signaling pathway, and suppressing Pb accumulation via interference on the binding of Pb to albumin.

show abstract

Interaction mechanism of flavonoids with whey protein isolate: A spectrofluorometric and theoretical investigation

Cited by 38 publications

References 31 publications

Interaction mechanism of icariin and whey protein based on spectrofluorimetry and molecular docking

Interaction mechanism of icariin and whey protein based on spectrofluorimetry and molecular docking

Exploring the binding effects and inhibiting mechanism of hyperoside to lipase using multi-spectroscopic approaches, isothermal titration calorimetry, inhibition kinetics and molecular dynamics

Epicatechin attenuates lead (Pb)-induced cognitive impairment in mice: regulation on Nrf2 signaling pathway, and interference on the interaction between Pb with albumin

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