Interaction network of the mitochondrial outer membrane protein Mcp3

Sinzel, Monika; Zeitler, Andreas; Dimmer, Kai Stefan

doi:10.1002/1873-3468.13243

Cited by 3 publications

(3 citation statements)

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“…Overexpression of none of these proteins resulted in a growth defect of psd1 Δ cells (Figure 5C). Additionally, we tested the overexpression of the third Mdm10-complementing protein, Mcp3, which was recently identified as an outer membrane component (Sinzel et al , 2016, 2018). Again, we did not observe an effect on the growth of cells lacking Psd1 (Figure 5C).…”

Section: Resultsmentioning

confidence: 99%

“…Although other sites of contact for MOM and MIM have been proposed, such as the interaction of the translocase of the outer membrane (TOM) and translocase of the inner membrane (TIM) machineries (Waegemann et al , 2015), their relevance to mitochondrial lipid trafficking is unknown. Recently, we demonstrated that another MOM protein, Mcp3, which functions as a weak suppressor of loss of ERMES function, interacts physically with the MIM protein Aim19 (Sinzel et al , 2018).…”

Section: Introductionmentioning

confidence: 99%

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The mitochondrial intermembrane space–facing proteins Mcp2 and Tgl2 are involved in yeast lipid metabolism

Odendall

Backes

Tatsuta

et al. 2019

MBoC

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Mitochondria are unique organelles harboring two distinct membranes, the mitochondrial inner and outer membrane (MIM and MOM, respectively). Mitochondria comprise only a subset of metabolic pathways for the synthesis of membrane lipids; therefore most lipid species and their precursors have to be imported from other cellular compartments. One such import process is mediated by the ER mitochondria encounter structure (ERMES) complex. Both mitochondrial membranes surround the hydrophilic intermembrane space (IMS). Therefore, additional systems are required that shuttle lipids between the MIM and MOM. Recently, we identified the IMS protein Mcp2 as a high-copy suppressor for cells that lack a functional ERMES complex. To understand better how mitochondria facilitate transport and biogenesis of lipids, we searched for genetic interactions of this suppressor. We found that MCP2 has a negative genetic interaction with the gene TGL2 encoding a neutral lipid hydrolase. We show that this lipase is located in the intermembrane space of the mitochondrion and is imported via the Mia40 disulfide relay system. Furthermore, we show a positive genetic interaction of double deletion of MCP2 and PSD1, the gene encoding the enzyme that synthesizes the major amount of cellular phosphatidylethanolamine. Finally, we demonstrate that the nucleotide-binding motifs of the predicted atypical kinase Mcp2 are required for its proper function. Taken together, our data suggest that Mcp2 is involved in mitochondrial lipid metabolism and an increase of this involvement by overexpression suppresses loss of ERMES.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The mitochondrial intermembrane space–facing proteins Mcp2 and Tgl2 are involved in yeast lipid metabolism

Odendall

Backes

Tatsuta

et al. 2019

MBoC

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“…In S. cerevisiae Fzo1 is an important mitochondrial integral protein involved specifically in the fusion of outer membranes which promotes mitochondrial fusion. Initiation of membrane fusion in other organelles requires small GTPases and SNAREs whereas in mitochondria dynamin-like large GTPases and the mitofusins (Mfn1 and Mfn2 in mammals; Fzo1 in yeast) are required [62,63,115,116]. Like Dnm1, Fis1 (fission 1) proteins have been shown to be essential for mitochondrial fission after forming a complex with each other to induce mitophagy and promote cell apoptosis [73,117].…”

Section: Mitochondrial Fission and Fusionmentioning

confidence: 99%

Autophagy related genes mediated mitophagy in yeast, mammals and higher plants

Muhammad Riaz,

Razia Sultana,

Javed Ahmad

et al. 2024

Cell Mol Biol (Noisy-le-grand)

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Autophagy is classified into macro-autophagy and micro-autophagy. Two major types of autophagy in the complex eukaryotic organism are microautophagy and macroautophagy. During microautophagy, cytoplasmic components that need to be degraded are taken up by lysosomes in animals and by vacuole in yeast and plants via the invagination of tonoplast. While macroautophagy is initiated after the formation of a cup-shaped membrane structure, a phagophore develops at cargo that grows in size and is sealed by double-membrane vesicles to form autophagosome; a generalized mechanism for degradation of the organelle. Autophagic removal of damaged mitochondria is a conserved cellular process to maintain a healthy mitochondrion called Mitophagy. In plants and animals, mitophagy has crucial roles in stress responses, senescence, development, and programmed cell death. Mitophagy appears in mammals, fungi, and plants but many genes that controlled mitophagy are absent from plants. Numerous studies have been conducted by using ATG mutants for the identification of functional roles of Autophagy Related Genes (ATG) required during the autophagy process at various steps like; auto phagosome formation, ATG protein recruitment, etc. The role of more than 25 ATG genes in mitophagy has been discussed in this review paper. The main parameters, reviewed and summarized in this review paper, are the name of species, common name, function, domain, deletion, induction, and localization of these autophagy-related genes in the cell. This review will facilitate the students, researchers, and academics for their further research insights.

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