Interaction of a plasma-sprayed hydroxyapatite coating in contact with human osteoblasts and culture medium

Abstract

The loss of calcium from plasma-sprayed calcium phosphate ceramics (CPCs) on bioinert metal substrate (Ti-6Al-4V) immersed in cell culture medium with or without human osteoblast culture was measured. The ceramics were a CPC and a duplex system composed of a CPC layer on an alumina coating. The dissolution of calcium compounds was monitored by measuring the calcium leaked from the coatings into the culture medium in 15 days. Calcium was measured by flame photometry. The surfaces of the ceramics exposed to the culture medium and in contact with osteoblasts were analysed by X-ray diffraction (XRD). The dissolution process occurred in the first 6 days of contact, but the calcium released into the culture medium was only a small fraction of the calcium content of the coatings. The presence or absence of osteoblasts on the surface of the ceramics did not make significant difference for the calcium release. The XRD spectra of the ceramics before and after immersion and in contact with cells did not show a significant change in the compounds of the coatings.

In vitro osteogenic potential of human bone marrow stromal cells cultivated in porous scaffolds from mineralized collagen

“…Some studies21, 23 suggest, that the Ca 2+ uptake of HA scaffolds is caused by the enhanced absorption of proteins which in turn bind Ca 2+ , acting as nucleating agents of mineralisation. On the other hand several HA‐containing ceramics are reported to release Ca 2+ ions 19, 25. This varying behavior could be addressed to differences in physicochemical properties of HA like crystallinity and particle size.…”

“…On the other hand several HA-containing ceramics are reported to release Ca 2þ ions. 19,25 This varying behavior could be addressed to differences in physicochemical properties of HA like crystallinity and particle size. To examine cell response to the HA collagen surface of the porous 3D scaffolds, enriching with serum proteins and calcium over the cell-cultivation period, we investigated the proliferation of osteogenic induced as well as non-induced hBMSC on these scaffolds.…”

Color tone and interfacial microstructure of white oxide layer on commercially pure Ti and Ti–Nb–Ta–Zr alloys

“…Thus, coating Ti alloys with a polymer or a ceramic on metallic dental devices is one of the possible techniques to conceal its metallic color or improve osseoconnectivity. To improve corrosion resistance 7,8) or to enhance osseointegration and regeneration of the bone tissue on a surface, 9,10) surface coating of Ti with an oxide layer, calcium phosphates such as hydroxyapatite [11][12][13] or other ceramics 9,14,15) has been investigated. 4,16) In addition, as mentioned above, the shade of color on dental devices such as an artificial tooth or orthodontic devices is as important as corrosion resistance and mechanical properties for improving the patients' quality of life (QOL).…”

A cross‐linking model for estimating Young's modulus of artificial bone tissue grown on carbon nanotube scaffold

“…HAp has the ability to bond chemically with living bone tissue owing to its chemical, compositional, biological, and crystal structural similarities to native apatite in the human skeleton 37–39. Furthermore, HAp bioactivity and biocompatibility encourage osteoblast adhesion and proliferation 39–41. However, brittle HAp is weak in tensile strength and offers a low fracture toughness in comparison to natural bone, and is thus unsuitable for main load bearing sites 42.…”