Interaction of a Tat Substrate and a Tat Signal Peptide with Thylakoid Lipids at the Air–Water Interface

Kerth, Andreas; Brehmer, Tina; Meister, Annette; Hanner, Peter; Jakob, Mario; Klösgen, Ralf Bernd; Blume, Alfred

doi:10.1002/cbic.201100458

Cited by 6 publications

(2 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This model includes the accepted current model and only extends it by the herein described TatA interaction. Tat substrates will first interact with membranes [56,57,58,59,60] and in E . coli , the first Tat component encountered by membrane-interacting Tat substrates is likely to be TatA.…”

Section: Discussionmentioning

confidence: 99%

TatBC-Independent TatA/Tat Substrate Interactions Contribute to Transport Efficiency

et al. 2015

View full text Add to dashboard Cite

The Tat system can transport folded, signal peptide-containing proteins (Tat substrates) across energized membranes of prokaryotes and plant plastids. A twin-arginine motif in the signal peptide of Tat substrates is recognized by TatC-containing complexes, and TatA permits the membrane passage. Often, as in the model Tat systems of Escherichia coli and plant plastids, a third component – TatB – is involved that resembles TatA but has a higher affinity to TatC. It is not known why most TatA dissociates from TatBC complexes in vivo and distributes more evenly in the membrane. Here we show a TatBC-independent substrate-binding to TatA from Escherichia coli, and we provide evidence that this binding enhances Tat transport. First hints came from in vivo cross-linking data, which could be confirmed by affinity co-purification of TatA with the natural Tat substrates HiPIP and NrfC. Two positions on the surface of HiPIP could be identified that are important for the TatA interaction and transport efficiency, indicating physiological relevance of the interaction. Distributed TatA thus may serve to accompany membrane-interacting Tat substrates to the few TatBC spots in the cells.

show abstract

Section: Discussionmentioning

confidence: 99%

TatBC-Independent TatA/Tat Substrate Interactions Contribute to Transport Efficiency

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The three major components that are unique in plants are glyceroglycolipids, i.e., monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), and sulfoquinovosyldiacylglycerol (SQDG). Some studies report on the use of glyceroglycolipid-containing vesicles, − black lipid membranes, and lipid monolayers at the air–water interface and deposited on a solid support by the Langmuir–Blodgett technique. − However, to the best of our knowledge, supported lipid bilayers (SLBs) composed of mixtures of all three of these glyceroglycolipids have not been produced. Although SLBs lack a large internal compartment as compared to vesicles, they have the advantage of being planar and long-term stable .…”

Section: Introductionmentioning

confidence: 99%

3D-Membrane Stacks on Supported Membranes Composed of Diatom Lipids Induced by Long-Chain Polyamines

Gräb¹,

Abacilar

Daus

et al. 2016

Langmuir

View full text Add to dashboard Cite

Long-chain polyamines (LCPAs) are intimately involved in the biomineralization process of diatoms taking place in silica deposition vesicles being acidic compartments surrounded by a lipid bilayer. Here, we addressed the question whether and how LCPAs interact with lipid membranes composed of glycerophospholipids and glyceroglycolipids mimicking the membranes of diatoms and higher plants. Solid supported lipid bilayers and monolayers containing the three major components that are unique in diatoms and higher plants, i.e., monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), and sulfoquinovosyldiacylglycerol (SQDG), were prepared by spreading small unilamellar vesicles. The integrity of the membranes was investigated by fluorescence microscopy and atomic force microscopy showing continuous flat bilayers and monolayers with small protrusions on top of the membrane. The addition of a synthetic polyamine composed of 13 amine groups separated by a propyl spacer (C3N13) results in flat but three-dimensional membrane stacks within minutes. The membrane stacks are connected with the underlying membrane as verified by fluorescence recovery after photobleaching experiments. Membrane stack formation was found to be independent of the lipid composition; i.e., neither glyceroglycolipids nor negatively charged lipids were required. However, the formation process was strongly dependent on the chain length of the polyamine. Whereas short polyamines such as the naturally occurring spermidine, spermine, and the synthetic polyamines C3N4 and C3N5 do not induce stack formation, those containing seven and more amine groups (C3N7, C3N13, and C3N18) do form membrane stacks. The observed stack formation might have implications for the stability and expansion of the silica deposition vesicle during valve and girdle band formation in diatoms.

show abstract

IRRA-Spektroskopie: Wechselwirkung von Proteinen mit Lipidmonoschichten

Kerth

Blume

2012

Biospektrum

View full text Add to dashboard Cite

Interaction of a Tat Substrate and a Tat Signal Peptide with Thylakoid Lipids at the Air–Water Interface

Cited by 6 publications

References 68 publications

TatBC-Independent TatA/Tat Substrate Interactions Contribute to Transport Efficiency

TatBC-Independent TatA/Tat Substrate Interactions Contribute to Transport Efficiency

3D-Membrane Stacks on Supported Membranes Composed of Diatom Lipids Induced by Long-Chain Polyamines

IRRA-Spektroskopie: Wechselwirkung von Proteinen mit Lipidmonoschichten

Contact Info

Product

Resources

About