Interaction of ACC deaminase and antioxidant enzymes to induce drought tolerance in Enterobacter cloacae 2WC2 inoculated maize genotypes

Maqbool, Saman; Amna, Amna; Maqbool, Ammarah; Mehmood, Shehzad; Suhaib, Muhammad; Sultan, Tariq; Munis, Muhammad Farooq Hussain; Rehman, Shafiqur; Chaudhary, Hassan Javed

doi:10.30848/pjb2021-3(28)

Cited by 18 publications

(10 citation statements)

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“…Moreover, the maize seedlings inoculated with Enterobacter sp. exhibited significantly higher antioxidant activity than uninoculated control, also reported by Maqbool et al (2021). The enhanced expression of ROS-scavenging antioxidant enzymes in PGPB-inoculated plants could be associated to the ability of bacterial inoculants to exhibit increased ACCdeaminase activity, siderophore production, and phosphate solubilization under adverse conditions (Gururani et al 2013).…”

Section: Discussionmentioning

confidence: 54%

Comparative Effects of Individual and Consortia Plant Growth Promoting Bacteria on Physiological and Enzymatic Mechanisms to Confer Drought Tolerance in Maize (Zea mays L.)

Saleem

Nawaz

Hussain

et al. 2021

J Soil Sci Plant Nutr

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Mitigation strategies based on plant–microbe interactions to increase the performance of plants under water-deficit conditions are well documented. However, little is known about a suitable consortium of bacterial inoculants and underlying physiological and enzymatic events to improve drought tolerance in maize. We performed laboratory and pot experiments to understand the synergistic interactions among plant growth-promoting bacteria to alleviate the drought-induced damages in maize. Initially, ten bacterial strains were evaluated for their osmotic stress tolerance capacity by growing them in a media containing 0, 10, 20, and 30% polyethylene glycol (PEG-6000). Also, the seeds of a drought tolerant (NK-6654) and sensitive (SD-626) maize cultivar were inoculated with these bacterial strains in the first pot experiment to determine their effects on the growth and physiological processes. Later, in the second pot experiment, the best performing inoculants were selected to study the individual and synergistic effects of bacterial inoculation to confer drought tolerance in maize. Our findings showed that the inoculation with tolerant strains resulted in higher photosynthetic activity (25–39%), maintenance of leaf water status (14–18%) and pigments (27–32%), and stimulation of antioxidant machinery (28–38%) than no inoculation in water-stressed maize seedlings. Moreover, the treatment with bacteria consortia further stimulated the drought protective mechanisms and resulted in higher efficiency of photosynthetic (47–61%) and antioxidant systems (42–62%) than the individual inoculants under water-deficit conditions. We conclude that the inoculation with microbial consortia regulates water uptake, photosynthetic performance, and stress metabolites to minimize drought-induced damages in maize.

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Section: Discussionmentioning

confidence: 54%

Comparative Effects of Individual and Consortia Plant Growth Promoting Bacteria on Physiological and Enzymatic Mechanisms to Confer Drought Tolerance in Maize (Zea mays L.)

Saleem

Nawaz

Hussain

et al. 2021

J Soil Sci Plant Nutr

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“…The increase in yield parameters and biomass of okra plants treated with PBR in normal (S1 and S2) and saline (S3) soil could be attributed to the bacterial strain's plant growth‐promoting properties. Plant growth‐promoting rhizobacteria promote plant growth under stress conditions by improving nutrient uptake, phytohormone activity, ACC deaminase activity which result in lower stress ethylene levels, and by producing siderophores, which improve plant iron uptake (Ferreira et al, 2019; Maqbool et al, 2021). In a previous study, the bacterial strain Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

“…"Ca contents" represents calcium (Ca) contents in plant dry matter of okra. "Na contents" represents sodium (Na) contents in plant dry matter of okra phytohormone activity, ACC deaminase activity which result in lower stress ethylene levels, and by producing siderophores, which improve plant iron uptake (Ferreira et al, 2019;Maqbool et al, 2021). In a previous study, the bacterial strain Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

“…These rhizobacteria associate with the plant roots and help the plants in improving their yield in normal agricultural soil and even in problem soils (metal contaminated soils, nutrient as well as water deficit soils and saline soils) through their stress tolerance mechanisms (Ahmad et al, 2018; Khan et al, 2021; Tahir, Ahmad, et al, 2019; Tahir, Khalid, et al, 2019). Numerous studies have reported the application of plant beneficial rhizobacteria as bioinoculants to various crops in pot as well as in field conditions which has shown a promising role in the growth and yield improvements in normal soils (Din et al 2020; Tahir et al, 2020) as well as under salt stress conditions (Ahmad et al, 2018; Maqbool et al, 2021). Nevertheless, in the presence of multiple success stories, commercialization of the plant beneficial rhizobacterial strains as biofertilizers has always been restricted and has not gained much attention.…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of organic-based carrier material for plant beneficial rhizobacteria application in okra under normal and salt-affected soil conditions

Tahir

Shahid

Nawaz

et al. 2022

Journal of Applied Microbiology

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Aims: Plant beneficial rhizobacteria (PBR) improve salt tolerance and plant yield in vegetable plants by producing 1-aminocyclopropane-1-carboxylate-deaminase, indole-3-acetic acid and phosphate solubilization. Organic-based carrier material is needed to ensure the PBR's uniform application, distribution, survival and functioning in a variety of fields. The PBR also use carbon present in the carrier as food and energy source. The selection of a suitable organic-based carrier material for the application of the PBR in normal and saline soils always has received less attention.The current study compared the PBR suitability of different organic-based carrier materials (biochar, biogas residues [BGRs] and coconut powder) and evaluated their effects on okra productivity under normal and saline soil conditions. Methods and Results:In a pot experiment, the PBR strain Bacillus sp. MR-1/2 (accession number, MG548383) was applied with/or without organic-based carrier materials to okra grown in three different soils: S1 (EC 1.0 dS m −1 ), S2 (EC 3.0 dS m −1 ) and S3 (EC 5.0 dS m −1 ). The experiment was set up in a completely randomized design with five replicates in factorial arrangement. Results indicated that in soil S1, PBR + BGR increased the number of pods per plant, plant dry weight and indole compounds by 64%, 68% and 17% while reduced the electrolyte leakage (ELL), malonaldehyde (MDA) contents and stress ethylene level by 17%, 55% and 38%, respectively over the PBR application without any carrier.

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“…Low synthesis of photosynthetic pigments could be due to a change in structural compounds and gas exchange factors that are blocked under stress conditions [46]. This reduction in chlorophyll content of the S. sesban plant could be due to the activation of chlorophyll and membrane damage [26,66]. On the other hand, the chlorophyll a (60.08%), chlorophyll b (48.38%), and total chlorophyll content (17.65%) were positively improved with the application of strain PM21 (Table 2).…”

Section: Discussionmentioning

confidence: 99%

Phytoremediation of Cadmium Contaminated Soil Using Sesbania sesban L. in Association with Bacillus anthracis PM21: A Biochemical Analysis

et al. 2021

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Sustainable food production to feed nine to 10 billion people by 2050 is one of the greatest challenges we face in the 21st century. Due to anthropogenic activities, cadmium (Cd) contamination is ubiquitous with deleterious effects on plant and soil microbiota. In the current study, the phytoremediation potential of Sesbania sesban L. was investigated in Cd-spiked soil inoculated with Bacillus anthracis PM21. The Cd-spiked soil drastically reduced important plant attributes; however, inoculation of B. anthracis PM21 significantly (p ≤ 0.05) enhanced root length (17.21%), shoot length (15.35%), fresh weight (37.02%), dry weight (28.37%), chlorophyll a (52.79%), chlorophyll b (48.38%), and total chlorophyll contents (17.65%) at the Cd stress level of 200 mg/kg as compared to the respective control. In addition, bacterial inoculation improved superoxide dismutase (11.98%), peroxidase (12.16%), catalase (25.26%), and relative water content (16.66%) whereas it reduced proline content (16.37%), malondialdehyde content (12.67%), and electrolyte leakage (12.5%). Inoculated plants showed significantly (p ≤ 0.05) higher Cd concentration in the S. sesban root (118.6 mg/kg) and shoot (73.4 mg/kg) with a translocation (0.61) and bioconcentration factor (0.36), at 200 mg/kg Cd. Surface characterization of bacteria through Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) predicted the involvement of various functional groups and cell surface morphology in the adsorption of Cd ions. Amplification of the CzcD gene in strain PM21, improved antioxidant activities, and the membrane stability of inoculated S. sesban plants conferred Cd tolerance of strain PM21. In addition, the evaluated bacterial strain B. anthracis PM21 revealed significant plant growth-promoting potential in S. sesban; thus, it can be an effective candidate for phyto-remediation of Cd-polluted soil.

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Interaction of ACC deaminase and antioxidant enzymes to induce drought tolerance in Enterobacter cloacae 2WC2 inoculated maize genotypes

Cited by 18 publications

References 0 publications

Comparative Effects of Individual and Consortia Plant Growth Promoting Bacteria on Physiological and Enzymatic Mechanisms to Confer Drought Tolerance in Maize (Zea mays L.)

Comparative Effects of Individual and Consortia Plant Growth Promoting Bacteria on Physiological and Enzymatic Mechanisms to Confer Drought Tolerance in Maize (Zea mays L.)

Efficacy of organic-based carrier material for plant beneficial rhizobacteria application in okra under normal and salt-affected soil conditions

Phytoremediation of Cadmium Contaminated Soil Using Sesbania sesban L. in Association with Bacillus anthracis PM21: A Biochemical Analysis

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