Interaction of aerobic soil bacteria with plutonium(VI)

Abstract

Plutonium / Bacillus sphaericus / Pseudomonas stutzeri / Biosorption / Absorption spectroscopy / ActinidesSummary. We studied the interaction of Pu(VI) with Pseudomonas stutzeri ATCC 17588 and Bacillus sphaericus ATCC 14577, representatives of the main aerobic groups of soil bacteria present in the upper soil layers. The biosorption studies have shown that these soil bacteria accumulate high amounts of Pu(VI). The relative sorption efficiency toward Pu(VI) related to the amount of biomass used decreased with increasing biomass concentration due to increased agglomeration of the bacteria resulting in a decrease of the number of available complexing groups. Spores of Bacillus sphaericus showed a higher biosorption than the vegetative cells at low biomass concentration which decreased significantly with increasing biomass concentration. At higher biomass concentrations (> 0.7 g/L), the vegetative cells of both strains and the spores of B. sphaericus showed comparable sorption efficiencies. Investigations on the pH dependency of the biosorption and extraction studies with 0.01 M EDTA solution have shown that the biosorption of plutonium is a reversible process and the plutonium is bound by surface complexation. Optical absorption spectroscopy showed that one third of the initially present Pu(VI) was reduced to Pu(V) after 24 hours. Kinetic studies and solvent extraction to separate different oxidation states of Pu after contact with the biomass provided further information on the yield and the kinetics of the bacteriamediated reduction. Long-term studies showed that also 16% of Pu(IV) was formed after one month. The slow kinetics of this process indicate that under our experimental conditions the Pu(IV) was not a produced by microbial reduction but seemed to be rather the result of the disproportionation of the formed Pu(V) or autoreduction of Pu(VI).

Interactions of Plutonium with Pseudomonas sp. Strain EPS-1W and Its Extracellular Polymeric Substances

“…Microorganisms are associated with a variety of radioactive materials at U.S. Department of Energy contaminated sites (14,15), and it is apparent that Pu speciation and solubility can be affected by microbial activity (16)(17)(18)(19)(20)(21). Previous studies of Pseudomonas stutzeri and Bacillus sphaericus reported a sorption affinity of nearly 100 g Pu/g dry cell weight for Pu(VI) to the surface of vegetative cells and spores (20).…”

“…Previous studies of Pseudomonas stutzeri and Bacillus sphaericus reported a sorption affinity of nearly 100 g Pu/g dry cell weight for Pu(VI) to the surface of vegetative cells and spores (20). Further investigations of Pu on B. sphaericus by extended X-ray absorption fine-structure (EXAFS) spectroscopy revealed that an insoluble Pu(VI) phosphate complex can form on the cell surface (22).…”

Biology of Pseudomonas stutzeri

“…These siderophores ensure the availability of essential metals, such as cobalt, copper, iron, and nickel (63,64,224,313,345). Furthermore, several P. stutzeri strains have been described due to their high biosorption potential and resistance to metals such as aluminum (422), chromium (12,172), cobalt (172), copper (69,215), germanium (370), lead (215), manganese (172), nickel (272,346), plutonium (255), selenium (157), silver (135), thallium (172), titanium (38), uranium (172), vanadium (172), and zinc (34,172,215).…”

Cs and Pu migration through engineered and natural barriers

“…In addition, the interaction with the bacteria can cause changes in the oxidation state of plutonium under environmental conditions [17,18]. The published material provides much useful information on the mobility of plutonium, but some of these publications are contradictory, especially regarding the oxidation state and speciation of plutonium at low pH values, and also the data obtained from the experiments conducted without the determination of the oxidation state of plutonium.…”