2002
DOI: 10.1152/ajpheart.00558.2001
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Interaction of albumin with the endothelial cell surface

Abstract: Endothelial cells (EC) are covered with cell-borne proteoglycans and glycoproteins. Blood plasma proteins (e.g., albumin) adsorb to this glycocalyx forming a complex endothelial surface layer (ESL). We determined the molecular mobility of albumin by electron spin resonance (ESR) in the presence and absence of ECs to analyze interactions with the ESL. Albumin was spin labeled with 5- or 12-4,4-dimethyloxazolidine- N-oxyl (DOXYL)-stearic acid yielding information on the mobility of the molecular surface (5-DOXYL… Show more

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Cited by 38 publications
(25 citation statements)
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“…Related to this, protein-facilitated metabolism is probably a consequence of an ionic interaction process between the extracellular protein-drug complex and hepatocyte cell surface, and that process can be very rapid. 13,37 Consequently, this may help the system to reach distributional equilibrium more rapidly and facilitate a homogenous dispersion of the drug, and hence explain why it could be worth converting fu p to fu liver at the whole-organ level to be in agreement with the wellstirred model. In addition, each ionic interaction between the bound drug (plasma protein-drug complex) and the hepatocyte cell surface would result in an additional unbound drug molecule available for uptake, and hence a greater intracellular concentration in vivo (and CL int ).…”
Section: Estimation Of Fu Livermentioning
confidence: 88%
“…Related to this, protein-facilitated metabolism is probably a consequence of an ionic interaction process between the extracellular protein-drug complex and hepatocyte cell surface, and that process can be very rapid. 13,37 Consequently, this may help the system to reach distributional equilibrium more rapidly and facilitate a homogenous dispersion of the drug, and hence explain why it could be worth converting fu p to fu liver at the whole-organ level to be in agreement with the wellstirred model. In addition, each ionic interaction between the bound drug (plasma protein-drug complex) and the hepatocyte cell surface would result in an additional unbound drug molecule available for uptake, and hence a greater intracellular concentration in vivo (and CL int ).…”
Section: Estimation Of Fu Livermentioning
confidence: 88%
“…25,28 This finding led to the "lymph flow paradox": Net capillary filtration rate calculated from tissue-averaged Starling forces based on the almost identical colloid osmotic forces between interstitial and intravascular spaces is much greater than the observed tissue lymph production. 26 But it is not the interstitial colloid that is important: The endothelial glycocalyx has a high colloid osmotic force due to retained plasma proteins, 29,30 whereas directly behind this structure, the protein concentration is normally very low, much lower than in tissue interstitial spaces. 25,26 As a result, the endothelial glycocalyx is the main structure that provides a colloid osmotic gradient and thus prevents tissue edema.…”
Section: Dimension and Physiological Role Of The Endothelial Glycocalyxmentioning
confidence: 99%
“…UNDER PHYSIOLOGICAL CONDITIONS, the endothelial barrier plays an important role in homeostasis and regulates the movement of fluid and solutes across the vessel wall (9,28,34,46,47). The ease of fluid movement across the endothelial barrier is described by the hydraulic conductivity (L p ), and changes in L p likely provide an assessment of barrier exchange function for water-soluble solutes (27).…”
mentioning
confidence: 99%