Interaction of Antagonistic Bacteria that Effective to Control Rice Bacterial Leaf Blight Disease with Agricultural Chemicals and Bio-products

Abstract

This research aimed to study the compatibility of antagonistic bacteria that effective to control rice bacterial leaf blight disease, including Bacillus pumilus FDKF5 and Bacillus sp. IKM1 with agricultural chemicals and bio-products. Compatibility test of antagonistic bacteria with pesticides by using poison plate method showed that B. pumilus FDKF5 and Bacillus sp. IKM1 were able grow together with eight pesticides including 5 types of insecticide such as Conbalis (pyridaben 20% WP), Centaur (imidachloprid 70% WG), Posch (carbosulfan 20% W/V EC), Microthiol sulfur (sulfur 80% WG) and Abamectin (abamectin 1.8% W/V EC) and 3 types of fungicide including Terraclor Super-X (etridiazole 6% + quintozene 4% W/V EC), Fundasol 50 (benomyl 50% WP) and Octave (prochloraz 50% WP). However, streptomycin sulfate bactericide was inhibited their growth. Co-culture test of antagonistic bacteria with bio-products by using dual culture technique showed that Trichoderma and Beauveria bio-products could overgrowth on colonies of antagonistic bacteria but did not inhibit their growth. While, Metarhizium bio-products could inhibit the growth of both antagonistic bacteria. Dual culture of antagonistic bacteria with Bacillus bio-product showed that Bacillus sp. IKM1 could grow very well while B. pumilus FDKF5 could grow moderately.