Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins

Mizushima, Yutaka; Kobayashi, Mika

doi:10.1111/j.2042-7158.1968.tb09718.x

Cited by 468 publications

(322 citation statements)

References 5 publications

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“…The inflammatory drugs (salicylic acid, phenylbutazone etc) have shown dose dependent ability to thermally induced protein denaturation. 26 Similar results were observed from many reports from plant extract. 27 The extracts may possibly inhibit the release of lysosomal content of neutrophils at the site of inflammation.…”

Section: Anti-inflammatory Assaysupporting

confidence: 81%

Phytochemical analysis and in vitro antioxidant, antimicrobial, anti-inflammatory and cytotoxicity activities of wood rotting fungi, Trametes ochracea

Govindappa¹,

Roshan²,

Nithi³

et al. 2015

Phcog J

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Objective: The present research was aimed to identify the metabolites in the methaolic and hexane extract of Trametes ochracea and evaluated these extracts to know their in vitro biological activities. Materials and methods: Two solvent extracts of T. ochracea was subjected to phytochemical analysis. The two solvent extracts were subjected to in vitro biological activity viz., antioxidant, anti-inflammatory and cytoxicity assays. Results: The methanol extract yielded important phytochemicals viz., saponins, flavonoids, alkaloids, steroids, phenols and tannins compared to hexane. The methanolic extract has shown strong antioxidant activity in all tested methods. The methanol extract was effectively inhibited the heat induced hemolysis, antilipoxygenase activity and also stabilized the membrane, avoided the membrane denaturation, proteinase and xanthine oxidase inhibition.The onion root meristametic cells were inhibited due to toxicity of methanol extract by possessing various cellular abnormalities in various stages of actively growing cells. The yeast cells were dead due to toxicity of methanol extract by possessing cell necrosis and also fragmented the cell DNA. Conclusion: The obtained results clearly indicates that Trametes ochracea methanol extract is having potent phytochemicals, which plays important role in antioxidant, anti-inflammatory, cytotoxicity assays. The further research is needed to identify the exact mechanism is by action of one or combination of active phytochemicals.

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Section: Anti-inflammatory Assaysupporting

confidence: 81%

Phytochemical analysis and in vitro antioxidant, antimicrobial, anti-inflammatory and cytotoxicity activities of wood rotting fungi, Trametes ochracea

Govindappa¹,

Roshan²,

Nithi³

et al. 2015

Phcog J

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“…Denaturation of proteins is a well documented cause of infl ammation in conditions like rheumatoid arthritis [Mizushima and Kobayashi 1968]. Thus protection against protein denaturation, which was the main mechanism of action of NSAIDS postulated by Mizushima and Kobayashi [1968] before the discovery of their inhibitory effect on cyclooxygenase by Vane [1971] may play an important role in the antirheumatic activity of NSAIDS.…”

Section: In-vitro Anti-infl Ammatory Activitymentioning

confidence: 99%

“…The protein denaturation inhibition activity of S. androgynus was determined as like Mizushima and Kobayashi [1968]. 450 μl of 5% BSA was incubated with test sample having different concentrations (or) Diclofenac (100 μg/ml).…”

Section: Anti-infl Ammatory Activitymentioning

confidence: 99%

New-vista in fi nding antioxidant and anti-infl ammatory property of crude protein extract from Sauropus androgynus leaf

Madhu

Manukumar²,

Basavaraju

2014

Acta Sci Pol Technol Aliment

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Aim. This study describes antioxidant and anti-infl ammatory properties of Sauropus androgynus leaf dialysed protein extract. Free radicals are implicated for many diseases including diabetes mellitus, arthritis, cancer, ageing etc. In the treatment of these diseases, antioxidant therapy has gained utmost importance. The main objective of the present study was to reveal the antioxidant and anti-infl ammatory potentiality from S. androgynus leaf dialysed protein extract. Material and methods. The antioxidant and anti-infl ammatory properties of S. androgynus studied using different models viz. hydroxyl radical scavenging, DPPH radical scavenging, reducing power assay, superoxide radical scavenging activity by alkaline DMSO and phosphomolybdenum antioxidant assays and in vitro antiinfl ammatory activity by inhibition of protein denaturation, membrane stabilisation test models carried out. Results. Antioxidant activity was estimated in dose dependent manner showed hydroxyl radical (55.62%), DPPH (50%), reducing power (0.286 Abs), alkaline DMSO (72.51%) and phosphomolybdenum (0.198 Abs) activity high at 50 μg/ml concentration compared to standard curcumin (62.31%), curcumin (56.61%), tannic acid (0.54 Abs), curcumin (75.38%) and vitamin E (0.15 Abs) respectively. In-vitro anti-infl ammatory activity by hypotonic induced model showed maximum protection (74.17%) compared to standard Acetylsalcylic acid (86.88%) at 100 μg/ml concentration and also in protein denaturation model protected protein denaturation maximum (83.60%) compared to standard Diclofenac (86.82%) at 100 μg/ml concentration respectively. Conclusion. The antioxidant property usually studied related to the polyphenols and fl avonoids present in the extract but present fi nding concluded that S. androgynus giving hint even potential proteins can also show responsible action effective against free radical mediated disease.

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“…8,9 The reaction mixture (0.5ml) consisted of 0.45ml bovine serum albumin (5% w/v aqueous solution) and 0.05ml of DSELE (100 , 200 , 400, 800 and 1000µg/ml) or Dexamethasone (100, 200, 400, 800 and 1000µg/ ml) in distilled water. All the above solutions were adjusted to pH 6.3 using 1N HCl.…”

Section: Inhibition Of Protein Denaturationmentioning

confidence: 99%

Anti-Inflammatory Activity of Ethanolic Leaf Extract of Dalbergia Sissoo In Vitro and In Vivo

Hajare¹

2017

ATROA

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Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins

Cited by 468 publications

References 5 publications

Phytochemical analysis and in vitro antioxidant, antimicrobial, anti-inflammatory and cytotoxicity activities of wood rotting fungi, Trametes ochracea

Phytochemical analysis and in vitro antioxidant, antimicrobial, anti-inflammatory and cytotoxicity activities of wood rotting fungi, Trametes ochracea

New-vista in fi nding antioxidant and anti-infl ammatory property of crude protein extract from Sauropus androgynus leaf

Anti-Inflammatory Activity of Ethanolic Leaf Extract of Dalbergia Sissoo In Vitro and In Vivo

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