Interaction of BES1 and LBD37 transcription factors modulates brassinosteroid-regulated root forging response under low nitrogen in arabidopsis

Abstract

Brassinosteriod (BR) plays important roles in regulation of plant growth, development and environmental responses. BR signaling regulates multiple biological processes through controlling the activity of BES1/BZR1 regulators. Apart from the roles in the promotion of plant growth, BR is also involved in regulation of the root foraging response under low nitrogen, however how BR signaling regulate this process remains unclear. Here we show that BES1 and LBD37 antagonistically regulate root foraging response under low nitrogen conditions. Both the transcriptional level and dephosphorylated level of BES1, is significant induced by low nitrogen, predominantly in root. Phenotypic analysis showed that BES1 gain-of-function mutant or BES1 overexpression transgenic plants exhibits progressive outgrowth of lateral root in response to low nitrogen and BES1 negatively regulates repressors of nitrate signaling pathway and positively regulates several key genes required for NO3- uptake and signaling. In contrast, BES1 knock-down mutant BES1-RNAi exhibited a dramatical reduction of lateral root elongation in response to low N. Furthermore, we identified a BES1 interacting protein, LBD37, which is a negative repressor of N availability signals. Our results showed that BES1 can inhibit LBD37 transcriptional repression on N-responsive genes. Our results thus demonstrated that BES1-LBD37 module acts critical nodes to integrate BR signaling and nitrogen signaling to modulate the root forging response at LN condition.

Transcriptome analysis of sesame (Sesamum indicum L.) reveal the LncRNAs and mRNAs regulatory network responding to low nitrogen stress

“…More and more evidence indicating that mRNA and lncRNAs play vital roles in stress response [55][56][57]. Although some functional genes such as bHLH, NAC, Dof, and MYB have been proven to participate in the response to low nitrogen stress in plants [58][59][60][61], only a few lncRNAs have been reported to respond to nitrogen stress at a whole transcriptome level, especially in sesame. To investigate low nitrogen treatment affects lncRNA expression in sesame, we analyzed the expression of DELs and DEGs in low nitrogen treatment and normal sesame roots through RNA-seq.…”

Ethylene‐MPK8‐ERF.C1‐PR module confers resistance against Botrytis cinerea in tomato fruit without compromising ripening

“…The split‐luciferase complementary assay was performed as described by Chai et al . (2022). The full‐length coding regions of SlERF.C1 , SlMPK8 , and SlMPK9 were cloned into the pCAMBIA‐1300‐nLuc or pCAMBIA‐1300‐cLUC vectors, respectively.…”

The heat response regulators HSFA1s promote Arabidopsis thermomorphogenesis via stabilizing PIF4 during the day

“…For the gene expression analysis, 5-day-old seedlings grown at 21°C under LD conditions were transferred into 28°C at ZT0 under LD, and the whole seedlings were harvested at ZT8 for RNA extraction. Extraction of total RNA from plant tissues followed previous protocols ( 80 ). Briefly, an RNeasy Mini Kit (Qiagen) was used to extract the total RNA from 50 mg of seedlings.…”

“…The RNA was reverse-transcribed into cDNA using the M-MLV Reverse Transcriptase Kit (Invitrogen) following the manufacturer’s instructions. Gene transcript levels were determined by reverse transcription–mediated quantitative PCR (RT-qPCR) on the CFX96 TM RealTime System (Bio-Rad) using 2 × perfect Start Green qPCR SuperMix (Transgene) as previously described ( 80 ). ACITN2 was used as the internal control.…”