Interaction of bivalent copper, nickel, manganese ions with native DNA and its monomers

Sorokin, V. A.; Valeev, Vladimir A.; Gladchenko, G. O.; Sysa, I. V.; Blagoi, Yuri P.; Volchok, I. V.

doi:10.1016/0162-0134(95)00176-x

Cited by 40 publications

(42 citation statements)

References 32 publications

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“…1(d)]. 5,13,14,16 In this model, Cu 2ϩ chelates with the N 7 and O 6 of guanine on one strand and the N 3 and O 2 of cytosine on the complementary strand. Formation of this complex requires significant changes in geometry.…”

Section: Introductionmentioning

confidence: 99%

“…It is thought that this interstrand complex affects the angle of the glycosidic bond of guanine from the anti to the syn conformation, thereby switching the type of hydrogen bonding between guanine and cytosine from WatsonCrick to Hoogsteen. 5,14 Although these structural modifications should alter the electronic circular dichroism (ECD) spectra significantly, such changes were not confirmed. 7,16 The possibilities of Cu 2ϩ chelate formation with the N 3 and O 2 of cytosine were shown for crystal and solution structures of metal complexes with cytidine.…”

Section: Introductionmentioning

confidence: 99%

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Vibrational circular dichroism and IR absorption of DNA complexes with Cu²⁺ ions

2003

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Vibrational circular dichroism (VCD) spectroscopy and simultaneous IR absorption measurements are applied to study the interaction of natural calf thymus DNA with Cu2+ ions at room temperature in a Cu2+ concentration range of 0-0.4M (a Cu2+/phosphate molar ratio [Cu]/[P] of 0-0.7). In some important instances, VCD provides more detailed insights than previous IR investigations whereas in several others it leads to the same interpretations. The Cu2+ ions bind to phosphate groups at a low metal concentration. Upon increasing the ion concentration, chelates are formed in which Cu2+ binds to the N7 of guanine (G) and a phosphate group. Detectable only by VCD, significant distortion of most guanine-cytosine (GC) base pairs occurs at a [Cu]/[P] ratio of 0.5 with only a minor affect on adenine-thymine (AT) base pairs, which favors a "sandwich" complex in which a Cu2+ ion is inserted between two adjacent guanines in a GpG sequence. The AT base pairs become significantly distorted when the metal concentration is increased to 0.7 [Cu]/[P]. A number of GC base pairs, which are possibly involved in sandwich complexes, remain stacked and paired even at 0.7 [Cu]/[P], preventing complete strand separation. The DNA secondary structure changes considerably from the standard B-form geometry at a [Cu]/[P] ratio of 0.4 and higher. A further transition to some intermediate conformation that is inconsistent with either the A- or Z-form or a completely denatured state is suggested in agreement with other works. In general, VCD proves to be a reliable indicator of the 3-dimensional structure of the DNA-metal ion complexes, which reveals structural details that cannot be deduced from the IR absorption spectra alone.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Vibrational circular dichroism and IR absorption of DNA complexes with Cu²⁺ ions

2003

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“…Metal-ion binding to nucleic acid has been studied by several techniques including conductimetry [3][4][5] (Mg 2+ -DNA, Ca 2+ -DNA), UV-Vis spectroscopy [5][6][7][8][9][10][11][12][13][14][15][16] (Mg 2+ -DNA, Ca 2+ -DNA, Cu 2+ -DNA, Mn 2+ -DNA, Ni 2+ -DNA, Cd 2+ -DNA, Hg 2+ -DNA, Cr 3+ -DNA, Zn 2+ -DNA), equilibrium dialysis [17][18][19] (Mg 2+ -DNA, Ca 2+ -DNA, Co 2+ -DNA, Zn 2+ -DNA) and X-ray crystallography [20][21][22][23] (Ca 2+ -DNA, Mg 2+ -DNA, Na + -DNA, Li + -DNA). However, all these techniques present considerable experimental difficulties or limitations.…”

Section: Introductionmentioning

confidence: 99%

Zinc binding to lambda phage DNA studied by voltammetric techniques

Souza¹,

Castro²,

Bloch³

2000

J. Braz. Chem. Soc.

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A interação do zinco com o DNA do fago λ foi investigada utilizando a voltametria de pulso diferencial e a voltametria cíclica. Os métodos baseiam-se no monitoramento direto da corrente de redução e oxidação do zinco na ausência e na presença do DNA viral. Curvas de titulação do Zn 2+ com o DNA do fago λ foram obtidas nos intervalos de concentração 3,57 x 10 -12 a 3,92 x 10 -11 mol L -1 e 6,97 x 10 -12 a 5,56 x 10 -11 mol L -1 . Estes dados foram utilizados para o cálculo da constante de dissociação do complexo e da estequiometria. O mecanismo da reação foi estudado utilizando a voltametria cíclica. Curvas do tipo I (corrente de oxidação do zinco) versus v 1/2 (raiz quadrada da velocidade de varredura) mostraram que o processo oxidação-redução do zinco deixa de ser difusional na presença do DNA do fago λ.Binding of zinc to lambda phage DNA was investigated by differential pulse voltammetry and cyclic voltammetry. These methods rely on the direct monitoring of reduction and oxidation current of zinc in the absence and presence of this virion DNA. Titration graphs of Zn 2+ with DNA were obtained in the concentration ranges from 3.57 x 10 -12 to 3.92 x 10 -11 mol L -1 and 6.97 x 10 -12 to 5.56 x 10 -11 mol L -1 . These data were used to calculate the dissociation constant of the complex and the stoichiometry. The mechanism of this reaction was studied by cyclic voltammetry through curves I (oxidation current of Zn 2+ ) versus v 1/2 (square root of scan rate). These curves showed that the oxidation-reduction process of Zn 2+ was not controlled by diffusion in the presence of lambda phage DNA.

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“…[26][27][28][29][30] The N7 position of guanine, which is an easily accessible site in the major groove of the DNA double helix structure, is the preferred metal binding site. 25,31 Many other sites, such as N3 of cytosine and N1 of adenine, were blocked by hydrogen bonding in a [Cu·DNA] complex.…”

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confidence: 99%

Structural Analysis of Cu Binding Site in [Cu(I)·d(CpG)·d(CpG)-2H]^-1Complex

Yujin

Jung²,

Kang³

et al. 2013

Bulletin of the Korean Chemical Society

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Interaction of bivalent copper, nickel, manganese ions with native DNA and its monomers

Cited by 40 publications

References 32 publications

Vibrational circular dichroism and IR absorption of DNA complexes with Cu²⁺ ions

Vibrational circular dichroism and IR absorption of DNA complexes with Cu²⁺ ions

Zinc binding to lambda phage DNA studied by voltammetric techniques

Structural Analysis of Cu Binding Site in [Cu(I)·d(CpG)·d(CpG)-2H]^-1Complex

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Interaction of bivalent copper, nickel, manganese ions with native DNA and its monomers

Cited by 40 publications

References 32 publications

Vibrational circular dichroism and IR absorption of DNA complexes with Cu2+ ions

Vibrational circular dichroism and IR absorption of DNA complexes with Cu2+ ions

Zinc binding to lambda phage DNA studied by voltammetric techniques

Structural Analysis of Cu Binding Site in [Cu(I)·d(CpG)·d(CpG)-2H]-1Complex

Contact Info

Product

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Vibrational circular dichroism and IR absorption of DNA complexes with Cu²⁺ ions

Vibrational circular dichroism and IR absorption of DNA complexes with Cu²⁺ ions

Structural Analysis of Cu Binding Site in [Cu(I)·d(CpG)·d(CpG)-2H]^-1Complex