Interaction of CD14 haplotypes and soluble CD14 on pulmonary function in agricultural workers

LeVan, Tricia D.; Smith, Lynette M.; Heires, Art J.; Mikuls, Ted R.; Meza, Jane L.; Weissenburger-Moser, Lisa; Romberger, Debra J.

doi:10.1186/s12931-017-0532-y

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…Membrane-bound CD14 is primarily made by monocytes, macrophages and granulocytes [15]. It is not normally found in endothelial or epithelial cells, such as the ones used in our study, which may instead depend on sCD14 for TLR4 activation [13]. A previous study indicated that serum is a key component of LPS activation (versus serum-free media) and that in the absence of serum, direct addition of CD14 (also found in serum) is sufficient for LPS stimulation of IL-8 production [3].…”

Section: Higher Serum Concentration Improves Lps Sensitizationmentioning

confidence: 75%

“…According to established literature on LPS stimulation, the efficiency of TLR4 activation by LPS is greatly increased by LPS-binding protein and CD14 [12][13][14]. CD14 is expressed as both a phosphatidylinositol-linked membrane-bound form (mCD14) and as a soluble circulating form (sCD14) [13]. Membrane-bound CD14 is primarily made by monocytes, macrophages and granulocytes [15].…”

Section: Higher Serum Concentration Improves Lps Sensitizationmentioning

confidence: 99%

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Various Factors Affect Lipopolysaccharide Sensitization in Cell Cultures

2020

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Commercially available lipopolysaccharide (LPS) is commonly used in research. Although protocols for its use are well established, we experienced a loss of LPS responsiveness in our cell cultures despite no obvious experimental changes. Our cell lines were stimulated with LPS and the media quantified for LPS responsiveness via an IL-8 ELISA. We discovered that the major cause of signal loss was differences in fetal bovine serum (FBS) formulation and concentration. One FBS formulation was notably better at eliciting an IL-8 signal than the second FBS, and 10% FBS in media was better at inducing LPS responsiveness than lower concentrations. We urge researchers to be aware of inherent variations in seemingly commonplace reagents as they may be unexpected sources of inconsistencies.

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Section: Higher Serum Concentration Improves Lps Sensitizationmentioning

confidence: 75%

Section: Higher Serum Concentration Improves Lps Sensitizationmentioning

confidence: 99%

Various Factors Affect Lipopolysaccharide Sensitization in Cell Cultures

2020

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“…В одному з досліджень встановлено, що при загостреннях ХОЗЛ відбувається підвищення sCD14 у крові -молекули, яка асоціюється зі старшим віком, жіночою статтю, поточним статусом куріння, гіпертензією, тригліцеридами, СРБ та ЦД2 [18,19]. Проте в роботі Shitole SG зазначається про відсутність зв'язку з ЦД2 [20].…”

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Effectiveness of rosuvastatin and pulmonary rehabilitation in patients with concurrent course of asthma, chronic obstructive pulmonary disease and diabetes mellitus type 2

Halytska,

Stupnytska

2023

Buk. Med. Herald

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The aim of this study was to assess the effectiveness of rosuvastatin and pulmonary rehabilitation in patients with asthma-COPD overlap (ACO) concurrent with type 2 diabetes (T2D).Material and methods. A total of 26 patients with ACO and T2D were examined, with an average age of 61 [55.6; 67.8]. In addition to standard therapy, patients in the main group were prescribed rosuvastatin and a pulmonary rehabilitation program. Treatment efficacy was assessed using questionnaires (CAT, ACQ, SGRQ), dyspnea severity scale (mMRC), BODE index, pulmonary function, bioimpedance analysis, 6-minute walk test, carbohydrate and lipid metabolism status, levels of 8-isoprostane, sCD14, CRP, stable nitric oxide (NO) metabolites - nitrite and nitrate ions, and endothelin-1 in the blood. Results. In the main group, a significant improvement was observed during the treatment course, including a 40% reduction in the BODE index (p<0.05), a 33.4% decrease in dyspnea on the mMRC scale (p<0.05), a 28.5% reduction in CAT, a 40.8% decrease in ACQ, and a 20.6% decrease in SGRQ. Other notable improvements included a 1.3% decrease in BMI, a 13% reduction in body fat percentage, a 23.5% decrease in visceral fat level, a 16% increase in patients' tolerance to physical activity, a 27.5% increase in FEV1 (p<0.05), an 8.1% decrease in fasting blood glucose (p<0.01), a 20% reduction in triglycerides (p<0.05), a 24.9% decrease in LDL cholesterol, and a 27.2% decrease in total cholesterol (p<0.05). Additionally, there was a 23.8% decrease in CRP levels (p<0.05), a two-fold reduction in blood 8-isoprostane levels (p<0.05), a 58% reduction in serum sCD14 levels, a 59.8% increase in stable nitric oxide metabolites (p<0.05), and a 55% decrease in endothelin-1 levels (p<0.05).Conclusions. The use of rosuvastatin and pulmonary rehabilitation significantly improves the quality of life for patients with the concurrent presentation of COPD, asthma, and T2D. It reduces the severity of dyspnea, enhances tolerance to physical activity, improves pulmonary function, lowers visceral fat levels and body fat percentage, enhances lipid profile, and improves endothelial function, all while concurrently reducing oxidative stress and systemic inflammation without worsening carbohydrate metabolism.

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Evaluating the Roles of sCD14 and sCD14-ST in Diagnosing COPD and Predicting an Acute Exacerbation of COPD

et al. 2022

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Aim To evaluate the roles of plasma soluble cluster of differentiation 14 (sCD14) and sCD14 subtype (sCD14-ST) in the diagnosis of chronic obstructive pulmonary disease (COPD) and in the prediction of an acute exacerbation of COPD (AECOPD). Methods We quantified the levels of white blood cell count (WBC), C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), interleukin (IL)-6, IL-8, sCD14, and sCD14-ST in patients with COPD and healthy controls. The relationships between sCD14 or sCD14-ST and inflammatory markers were analyzed in each group. We used receiver operating characteristics (ROC) curves to evaluate the potential roles of sCD14 and sCD14-ST in the diagnosis of COPD and in predicting AECOPD. Results A total of 62 subjects were recruited, including 15 controls and 47 COPD patients, with the latter including 32 stable COPD and 15 AECOPD. WBC, IL-8, sCD14, and sCD14-ST were significantly higher in COPD than in the controls (all P < 0.05). WBC, CRP, ESR, IL-6, IL-8, sCD14, and sCD14-ST were higher in AECOPD than in the controls (all P < 0.05). In the COPD group, sCD14 levels were positively correlated with WBC, IL-8, and sCD14-ST (P < 0.05), and sCD14-ST levels were positively correlated with WBC and IL-8 (P < 0.05). In the AECOPD group, sCD14 was positively correlated with WBC, CRP, IL-8, and sCD14-ST (P < 0.05); sCD14-ST was positively correlated with WBC, IL-6, and IL-8 (P < 0.05). Discrimination between COPD and controls was tested by calculating areas under the ROC curve (AUCs) for sCD14 and sCD14-ST showing scores of 0.765 (95% CI 0.648–0.883) and 0.735 (95% CI 0.537–0.933) respectively. Similarly, discrimination between AECOPD and controls using sCD14 and sCD14-ST showed scores of 0.862 (95% CI 0.714–1.000) and 0.773 (95% CI 0.587–0.960), respectively. Conclusion Our study suggests that the inflammatory markers sCD14 and sCD14-ST might play an important diagnostic role in COPD and help predict AECOPD.

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Interaction of CD14 haplotypes and soluble CD14 on pulmonary function in agricultural workers

Cited by 5 publications

References 41 publications

Various Factors Affect Lipopolysaccharide Sensitization in Cell Cultures

Various Factors Affect Lipopolysaccharide Sensitization in Cell Cultures

Effectiveness of rosuvastatin and pulmonary rehabilitation in patients with concurrent course of asthma, chronic obstructive pulmonary disease and diabetes mellitus type 2

Evaluating the Roles of sCD14 and sCD14-ST in Diagnosing COPD and Predicting an Acute Exacerbation of COPD

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