Interaction of cells with polymers

Smetana, Karel; Vacı́k, J.; Dvořánková, Barbora

doi:10.1002/masy.19961090114

Cited by 5 publications

(6 citation statements)

References 12 publications

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“…Nevertheless, soft (elastic) P(HEMA‐ co ‐EDMA) I/1 (2% EDMA and NaCl as a porogen) can be recommended as a mouse ES cell support, because out of all the slabs involved in the panel study the highest number of cells colonized the hydrogel. This is in accordance with the earlier observed high growth of epidermal cells containing a pool of epidermal stem cells on the surface of neat PHEMA 34…”

Section: Resultssupporting

confidence: 93%

“…Spreading of cells is essential for their correct attachment and early proliferation 33. In contrast, Smetana found nonstandard and altered adhesion to and spreading of fibroblasts on neat PHEMA and P(HEMA‐ co ‐methacrylic acid) materials in some cases 34. Nevertheless, soft (elastic) P(HEMA‐ co ‐EDMA) I/1 (2% EDMA and NaCl as a porogen) can be recommended as a mouse ES cell support, because out of all the slabs involved in the panel study the highest number of cells colonized the hydrogel.…”

Section: Resultsmentioning

confidence: 96%

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CD4+CD28− T cell expansion in granulomatosis with polyangiitis (Wegener's) is driven by latent cytomegalovirus infection and is associated with an increased risk of infection and mortality

Morgan

Pachnio

Begum

et al. 2011

Arthritis & Rheumatism

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Objective. Expanded populations of CD4؉ CD28؊ T cells with a cytotoxic phenotype have been repeatedly reported in patients with granulomatosis with polyangiitis (Wegener's) (GPA). In healthy individuals expansion of this T cell population follows cytomegalovirus (CMV) infection. We undertook this study to investigate whether CMV infection may be responsible for driving the expansion of CD4؉CD28؊ T cells in GPA patients and how this might relate to clinical features.Methods. Forty-eight GPA patients and 38 agematched healthy donors were included in the study. CMV-specific IgG in serum was detected by enzymelinked immunosorbent assay. Flow cytometric analysis was used to study T cell populations and phenotype. The presence of CMV in renal biopsy tissue from GPA patients was investigated by immunohistochemistry and polymerase chain reaction (PCR). Clinical information was obtained from patient records.Results. Populations of CD4؉CD28؊ T cells were only expanded in CMV-seropositive GPA patients and controls. In CMV-seropositive GPA patients we observed negative correlations between the percentages of CD4؉CD28؊ T cells and both the percentage of naive T cells and the glomerular filtration rate at presentation. There was a significant association between the percentage of CD4؉CD28؊ T cells and risk of infection and mortality. CMV could not be detected in renal tissue by PCR or immunohistochemistry. CMV seropositivity itself was not a risk factor for infection in a cohort of 182 patients with antineutrophil cytoplasmic antibody-associated vasculitis who had been recruited into clinical trials performed by the European Vasculitis Study Group.Conclusion. The expansion of CD4؉CD28؊ T cells in GPA patients is associated with CMV infection and leads to a reduction in the number of naive T cells in peripheral blood. Patients with expanded CD4؉CD28؊ T cells have significantly increased mortality and risk of infection.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 96%

CD4+CD28− T cell expansion in granulomatosis with polyangiitis (Wegener's) is driven by latent cytomegalovirus infection and is associated with an increased risk of infection and mortality

Morgan

Pachnio

Begum

et al. 2011

Arthritis & Rheumatism

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“…This suggests that the deposition of complement protein C3bi is very low and thereby the complement system is not very active on the -COOH functionalized surface. It has also been shown that implants bearing the anionic -COOH group are well tolerated by the immune system, 76 and has resulted in reduced fibrinogen adsorption. 77 The overall negative charge of the -COOH group repels plasma protein and reduces interactions with fibrotic cells.…”

Section: Discussionmentioning

confidence: 99%

Species and Density of Implant Surface Chemistry Affect the Extent of Foreign Body Reactions

et al. 2008

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Implant-associated fibrotic capsule formation presents a major challenge for the development of long term drug release microspheres and implantable sensors. Since material properties have been shown to affect in vitro cellular responses and also to influence short term in vivo tissue responses, we have thus assumed that the type and density of surface chemical groups would affect the degree of tissue responses to microsphere implants. To test this hypothesis, polypropylene particles with different surface densities of -OH and -COOH groups, along with the polypropylene control (-CH 2 groups) were utilized. The influence of functional groups and their surface densities on tissue reactions were analyzed using a mice subcutaneous implantation model. Our comparative studies included determination and correlation of the extents of fibrotic capsule formation, cell infiltration into the particles and recruitment of CD11b+ inflammatory cells for all of the substrates employed. We have observed major differences among microspheres coated with different surface functionalities. Surfaces with -OH surface groups trigger the strongest responses while -COOH rich surfaces prompt the least tissue reactions. However, variation of the surface density of either functional group has a relatively minor influence on the extent of fibrotic tissue reactions. The present results show that surface functionality can be used as a powerful tool to alter implant-associated fibrotic reactions and, potentially, to improve the efficacy and function of drug delivery microspheres, implantable sensors and tissue engineering scaffolds.

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“…some cases. 34 Nevertheless, soft (elastic) P(HEMA-co-EDMA) I/1 (2% EDMA and NaCl as a porogen) can be recommended as a mouse ES cell support, because out of all the slabs involved in the panel study the highest number of cells colonized the hydrogel. This is in accordance with the earlier observed high growth of epidermal cells containing a pool of epidermal stem cells on the surface of neat PHEMA.…”

Section: Cell Morphologymentioning

confidence: 99%

“…This is in accordance with the earlier observed high growth of epidermal cells containing a pool of epidermal stem cells on the surface of neat PHEMA. 34…”

Section: Cell Morphologymentioning

confidence: 99%

Functional polymer hydrogels for embryonic stem cell support

et al. 2005

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Embryonic stem (ES) cells are pluripotent cells with the ability to differentiate among all embryonic and adult cell lineages. Derivation of human ES cells opened up the way for treatment of many serious disorders by stem cell-based transplantation therapy. One of the most exciting challenges in development of transplantation therapies is to repair the damaged part of the organ or tissue by transplantation of undifferentiated ES cells or their differentiated derivatives within three-dimensional polymer scaffold. This method allows both renewal of structure and restoration of function of the organ. To address this issue, new polymer hydrogels were synthesized and tested. Cationic hydrogel slabs were synthesized by bulk radical copolymerization of 2-hydroxyethyl methacrylate (HEMA) and 2-(dimethylamino)ethyl methacrylate (DMAEMA) with ethylene dimethacrylate (EDMA) or 1-vinyl-2-pyrrolidone (VP) with N,N'-divinylethyleneurea (DVEU) or EDMA in the presence of saccharose or NaCl as a porogen. Swelling studies of the synthesized copolymers showed a high water content in the swollen state. Biocompatibility was studied with the use of feeder-independent mouse ES cell line D3. Cells grown either on the surface or inside synthesized polymer slabs suggest that the tested slabs are not toxic. The ability of ES cells to proliferate was only partially limited in PHEMA slabs crosslinked with EDMA compared with standard culture conditions. When cultured for a limited period of time, ES cells retained their undifferentiated state independently of properties of the hydrogel slabs, presence or absence of surface charges, type of crosslinking agent and matrix (PHEMA or PVP). Notably, prolonged culture in superporous hydrogel slabs initiated ES cell differentiation. Compared with unmodified PHEMA, the number of proliferating ES cells was still lower in the presence of cationic polymers.

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Interaction of cells with polymers

Cited by 5 publications

References 12 publications

CD4+CD28− T cell expansion in granulomatosis with polyangiitis (Wegener's) is driven by latent cytomegalovirus infection and is associated with an increased risk of infection and mortality

CD4+CD28− T cell expansion in granulomatosis with polyangiitis (Wegener's) is driven by latent cytomegalovirus infection and is associated with an increased risk of infection and mortality

Species and Density of Implant Surface Chemistry Affect the Extent of Foreign Body Reactions

Functional polymer hydrogels for embryonic stem cell support

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