Interaction of chlorhexidine with biomembrane models on glass ionomer by using the Langmuir–Blodgett technique

Unraveling the mechanism of octenidine and chlorhexidine on membranes: Does electrostatics matter?

“…Costalonga et al. ( 40 ) observed noticeable CHX activity in various dental surface models, showing that intrinsic interactions of the drug with the hydrophobic part of the lipid membrane led to disruption of the lipid organization at the interphase. Additionally, the secondary structure of the polypeptide model was changed as a result of CHX action.…”

“…Additionally, the secondary structure of the polypeptide model was changed as a result of CHX action. Moreover, the described interactions between lipid and protein moieties are believed to be present in membranes and may have a specific implication for understanding how antiseptics act on the bacterial membrane ( 40 ). Based on the available literature, it can be assumed that the effect of CHX is comprehensive.…”

Interactions of bioactive molecules & nanomaterials with Langmuir monolayers as cell membrane models

“…A similar conclusion was drawn for chlorhexidine, an antimicrobial agent mainly used in dentistry [166], which was made to interact with hybrid monolayers of DPPC and glucose oxidase to mimic the bacterial membrane. The purpose was to identify the membrane active sites…”

“…Adapted from reference[166].The importance of electrostatic interactions in exerting the biocidal activity has been shown for organic molecules possessing a quaternary ammonium group. Microbial cell death is attributed to the exchange of counterions between the cationic surface and negatively charged bacterial membranes, resulting in cell membrane disruption and release of intracellular contents.The biocide, fluorescent polymers possessing alkylammonium units such as poly(phenylene ethynylene) (PPE)-based conjugated polyelectrolytes (CPEs), were investigated in PG monolayers mimicking bacterial membranes [167].…”

Analytical methods for studying drug–biomembrane interactions

“…76 The insertion of the drug caused the surface pressure-area isotherms for a mixed protein-lipid monolayer to be shifted to higher lipid molecular areas, which was the fi rst indication of the action of chlorhexidine in the membrane model. Additional surface infrared spectroscopy analysis suggested there were intrinsic interactions of the drug with the hydrophobic part of the lipid, leading to a disruption of the lipid organization at the interface and to changes in the secondary structure of the protein used.…”