Interaction of citrinin and resveratrol and their effect on Caco-2 cell growth

Bovdisova, Ivana; Grabacka, Maja; Capcarová, Marcela

doi:10.5513/jcea01/17.4.1846

Cited by 2 publications

(1 citation statement)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CIT has been reported to reduce human HCT116 colon cancer cell viability after 36 h exposure at concentrations of 150 and 300 µM, with the identified LC50 being 300 µM [169]. Authors in [168] reported that CIT exposure also resulted in a decrease in Caco-2 cell viability at 399.6 and 999 µM after 48 h exposure. Cell apoptosis was induced by CIT via endoplasmic reticulum stress [169].…”

Section: In-vitro Cell Culture Systemsmentioning

confidence: 99%

In-Vitro Cell Culture for Efficient Assessment of Mycotoxin Exposure, Toxicity and Risk Mitigation

Karrow

Shandilya

et al. 2020

Toxins

View full text Add to dashboard Cite

Mycotoxins are toxic secondary fungal metabolites that commonly contaminate crops and food by-products and thus, animal feed. Ingestion of mycotoxins can lead to mycotoxicosis in both animals and humans, and at subclinical concentrations may affect animal production and adulterate feed and animal by-products. Mycotoxicity mechanisms of action (MOA) are largely unknown, and co-contamination, which is often the case, raises the likelihood of mycotoxin interactions. Mitigation strategies for reducing the risk of mycotoxicity are diverse and may not necessarily provide protection against all mycotoxins. These factors, as well as the species-specific risk of toxicity, collectively make an assessment of exposure, toxicity, and risk mitigation very challenging and costly; thus, in-vitro cell culture models provide a useful tool for their initial assessment. Since ingestion is the most common route of mycotoxin exposure, the intestinal epithelial barrier comprised of epithelial cells (IECs) and immune cells such as macrophages, represents ground zero where mycotoxins are absorbed, biotransformed, and elicit toxicity. This article aims to review different in-vitro IEC or co-culture models that can be used for assessing mycotoxin exposure, toxicity, and risk mitigation, and their suitability and limitations for the safety assessment of animal foods and food by-products.

show abstract

Section: In-vitro Cell Culture Systemsmentioning

confidence: 99%

In-Vitro Cell Culture for Efficient Assessment of Mycotoxin Exposure, Toxicity and Risk Mitigation

Karrow

Shandilya

et al. 2020

Toxins

View full text Add to dashboard Cite

show abstract

DNA damage in rat kidneys and liver upon subchronic exposure to single and combined ochratoxin A and citrinin

Rašić

Želježić

Kopjar

et al. 2019

WMJ

View full text Add to dashboard Cite

The study aimed to check whether ochratoxin A (OTA) and citrinin (CIT) increase DNA damage in the kidney and liver of male Wistar rats (alkaline comet assay), clarify the oxidative nature of DNA damage (hOGG1-modified comet assay), and verify whether resveratrol (RSV) could ameliorate OTA+CIT-induced genotoxicity. Rats were treated orally with OTA (0.125 and 0.250 mg/kg bodyweight (bw)) and CIT (2 mg/kg bw), OTA+CIT combinations and OTA+CIT+RSV (0.250+2+20 mg/kg bw) for 21 days. Both alkaline and hOGG1-modified comet assay showed that DNA damage was more severe in rat kidneys than in liver following mycotoxin treatment. Alkaline comet assay revealed a higher intensity of DNA damage, particularly as measured by tail intensity in the kidneys. Both tail length and tail intensity were OTA dose-dependent, but in combined OTA+CIT treatment these values were similar to CIT alone and lower than in animals treated with single OTA, possibly due to induction of apoptosis. hOGG1-modified comet showed that OTA+CIT evoked greater oxidative DNA damage than single mycotoxins. RSV did not reduce DNA damage measured by alkaline comet assay, but hOGG1-modified comet showed that RSV ameliorated OTA+CIT genotoxicity in the kidneys. Apart from oxidative stress, other mechanisms of DNA damage are involved in OTA and CIT genotoxicity. In rat kidneys RSV can reduce but not overcome oxidative DNA damage induced by combined OTA and CIT.

show abstract

Interaction of citrinin and resveratrol and their effect on Caco-2 cell growth

Cited by 2 publications

References 22 publications

In-Vitro Cell Culture for Efficient Assessment of Mycotoxin Exposure, Toxicity and Risk Mitigation

In-Vitro Cell Culture for Efficient Assessment of Mycotoxin Exposure, Toxicity and Risk Mitigation

DNA damage in rat kidneys and liver upon subchronic exposure to single and combined ochratoxin A and citrinin

Contact Info

Product

Resources

About