Interaction of Co, Mn, Mg and Al with d(GCGTACGC): a spectroscopic study

Rajan, M. T.; Champion, Dominique; Kumar, C. S.; Vishnuvardhan, Daesety; Rao, K. S.; Viswamitra, M. A.

doi:10.1007/bf00996304

Cited by 8 publications

(7 citation statements)

References 17 publications

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“…In general, metal ions such as Cu(II), Co(II), Mn(II) and Mg(II) bind to ds-DNA at both phosphate groups and base residues sites, and Al(III) binds to it at the former site. [36][37][38][39] Al(III) binding to ds-DNA at base residues sites was previously proposed by Karlik et al, 21 and testified to be the case by 27 Al NMR. 24 Our studies have shown that about 53.5% of Al(III) is probably binding to ds-DNA at phosphate groups sites, and the other 46.5% of Al(III) is kinetically united at base residues sites.…”

Section: Al(oh)3 Precipitation Analyzed By Icp-aes and Raman Spectramentioning

confidence: 85%

“…22,41 The 221 nm peak is due to the formation of a hydrogen bond. 37 The peaks at 245.6 and 274.2 nm are ascribed to the base pairs of ds-DNA. 42,43 After the addition of Al(III) from 5.0 × 10 -7 to 1.0 × 10 -5 M, the broad 274.2 nm peak inclined to shift to be more positive, and seemed to split to a shoulder peak at 281 nm.…”

Section: Al(oh)3 Precipitation Analyzed By Icp-aes and Raman Spectramentioning

confidence: 99%

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Electrochemical and Spectrometric Studies of Double-Strand Calf Thymus Gland DNA Denatured by Al(III) at Neutral pH

et al. 2009

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The interaction between double-strand calf thymus gland DNA (ds-DNA) and Al(III) was studied by using differential pulse voltammetry (DPV) at a hanging mercury drop electrode (HMDE), Raman spectrometry and circular dichroism (CD) spectra. It was shown that at neutral pH ds-DNA did not produce any cathodic peak at the HMDE in the potential window from -550 to -2000 mV vs. SCE. However, in the presence of Al(III), a cathodic peak was generated at about -1660 mV, which is ascribed to a reduction of adenine and cytosine residues of single denatured DNA (sd-DNA). It was concluded that ds-DNA was completely denatured to sd-DNA by Al(III) at a neutral pH. The apparent denaturing kinetic velocity constants of ds-DNA by Al(III) were derived from linear increases of the cathodic peak currents with time.3 ≥ 2 × 10 -26 , the precipitation of Al(OH)3 was observed and identified by the Raman spectrum, and inductively coupled plasma atomic emission spectrometry (ICP-AES). CD spectra showed that the B-type of structure conformations of ds-DNA and related sd-DNA did not change with the increment of Al(III) from 5.0 × 10 -7 to 1.0 10 -5 M, but the corresponding absorption strengths increased. The related physiological significances and possible applications of the observations were considered.

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Section: Al(oh)3 Precipitation Analyzed By Icp-aes and Raman Spectramentioning

confidence: 85%

Section: Al(oh)3 Precipitation Analyzed By Icp-aes and Raman Spectramentioning

confidence: 99%

Electrochemical and Spectrometric Studies of Double-Strand Calf Thymus Gland DNA Denatured by Al(III) at Neutral pH

et al. 2009

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“…It was earlier reported that Al did not alter the t m of DNA, and Mg was found to enhance the t m of genomic DNA [10]. Rajan et al [16] reported that Al did not alter the t m of the DNA sequence the metal ions as well as the base sequence of the DNA. The divalent metal ions Mg, Mn and Co with, relatively small ionic radii, strongly interact with the DNA [16].…”

Section: D(gcccatgggc)mentioning

confidence: 98%

“…Rajan et al [16] reported that Al did not alter the t m of the DNA sequence the metal ions as well as the base sequence of the DNA. The divalent metal ions Mg, Mn and Co with, relatively small ionic radii, strongly interact with the DNA [16]. Although these metals are required for a number of biological functions, at higher concentrations they are reported to change the handedness of DNA [1-6].…”

Section: D(gcccatgggc)mentioning

confidence: 98%

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Interaction of Co, Mn, Mg and Al with d(GCCCATGGC) and d(CCGGGCCCGG): a spectroscopic study

Champion

Kumar

Rajan

et al. 1998

Cellular and Molecular Life Sciences (CMLS)

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Spectroscopic study of the interactions of metal (CD) spectra of the oligomers in a concentration-depenions, Co, Mn, Mg and Al with d(GCCCATGGGC) and dent manner. The CD spectra of d(GCCCATGGGC) and d(CCGGGCCCGG) indicated B and Z forms of DNA, d(CCGGGCCCGG) revealed the following. Metal ions Mn, Al and Mg at the lowest concentrations enhanced respectively, in contrast to the A form observed in the the t m of oligomers, whereas Mn and Mg at higher crystal structures. Mg and Co at different ionic strength concentrations decreased the t m . Co enhanced the t m of induced Z-B transition in d(CCGGGCCCGG), while Al oligomers at higher concentrations. The studies have also at higher concentrations induced a Z-A transition. Mn indicated that Mn at lower concentrations displaced EtBr did not induce any transition. This is the first report to fluorescence, Mg and Co at moderate concentrations and show that Al causes structural transitions in sequenceAl only at higher concentrations. Addition of Co, Mn, specific oligomers and has strong binding ability with Mg and Al altered the bands of the circulars dichroism GC-rich euchromatin oligomers.Metal ions are known to be involved in the stabilization of DNA strands and in the catalytic activity of DNA polymerases [1]. Transition metals such as Mn and Co at higher ionic strength are found to be mutagenic and carcinogenic and also change the handedness of their DNA [1 -6]. Al is reported to be associated with neurological disorders [7] and causes cellular toxicity through its interaction with DNA in cells [8 -9]. Many data are available on the effect of metal ions on the melting behaviour of genomic DNA, uDNA, poly (A-T) and poly (G-C) [10 -12]. However, the precise mechanisms of binding of the metal ions to DNA are not yet clearly understood. Metal-binding studies on defined short sequences of DNA are also limited [13][14][15][16] and would be of interest in this connection. Recently, the crystal structures of the two oligomers d(CCGGGCCCGG) and d(GCCCATGGGC) were solved in our laboratory. Both structures crystallize isomorphously and are found to adopt the A-DNA conformation in the crystals. They exhibit interesting changes in their local conformation which are brought about by base sequence and crystal-packing forces. We report here the melting behaviour and spectroscopic analysis of the metal ion interactions of Mn, Co, Mg and Al with the two deoxyoligonucleotides which were carried out to understand their structure in the solution

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The Complexity of Aluminum-DNA Interactions: Relevance to Alzheimer’s and Other Neurological Diseases

Anitha

Rao

2002

Group 13 Chemistry II

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Interaction of Co, Mn, Mg and Al with d(GCGTACGC): a spectroscopic study

Cited by 8 publications

References 17 publications

Electrochemical and Spectrometric Studies of Double-Strand Calf Thymus Gland DNA Denatured by Al(III) at Neutral pH

Electrochemical and Spectrometric Studies of Double-Strand Calf Thymus Gland DNA Denatured by Al(III) at Neutral pH

Interaction of Co, Mn, Mg and Al with d(GCCCATGGC) and d(CCGGGCCCGG): a spectroscopic study

The Complexity of Aluminum-DNA Interactions: Relevance to Alzheimer’s and Other Neurological Diseases

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