Interaction of Copper(II) with Deoxyribonucleic Acid below 30°<sup>*</sup>

Bryan, Sara E.; Frieden, Earl

doi:10.1021/bi00861a012

Cited by 54 publications

(20 citation statements)

References 14 publications

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“…This confirms the conclusion that the loss of the hypochromicity induced by Cu2+ ions a t 25" is due to their interaction with the bases. A similar interaction of Cu2+ with salmon sperm DNA at room temperature was observed by Bryan and Frieden [15]. As revealed by Fig.…”

Section: Xpectrophotometric Titrationsupporting

confidence: 85%

“…Melting Behavior of iiethylated DNA-Cu2+ Complexes Since the role of GC base pairs for Cu2+ binding with DNA was predicted from temperature-denaturation measurements [3,6] and from several other indications [7,8,12,15,16], the chemical modification of GC pairs should influence the melting behavior of the DNA-Cu2+ complex. The results are illustrated in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, much lower Cu2+ concentrations are necessary to induce the transition as shown in Fig.8B at pH 4.06 and 4. 15. The cooperative transition occurs beginning between 0.5 and 1.5 Cu2+/2 DNA-P because of the low stability of highly protonated DNA [31].…”

Section: Xpectrophotometric Titrationmentioning

confidence: 99%

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Influence of Copper2+ Ions on the Denaturation and Spectral Properties of DNA and Methylated DNA

Zimmer¹,

Venner²

1970

Eur J Biochem

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Further indications have been obtained for the complex binding site of Cu2+ with DNA from difference spectra and titration of methylated and non-methylated DNA-Cuz+ complexes.The ultraviolet spectra of GC-rich DNAs show a shift in their absorption maxima of approx. 3 nm in the presence of Cu2+. A more pronounced displacement of the maxima is observed in the difference spectra of DNA-Cue+ complexes depending on the GC content.Methylation of DNA a t N-7 of guanine reduces the afsnity of DNA for Cu2+ association: the destabilizing effect of Cue+ on melting of the double helix is markedly diminished, and difference spectra are less affected by Cuz+.Spectrophotometric titration of various DNAs indicates binding of Cu2+ to the bases a t low ionic strength and at 25". The Cuzf-induced hyperchromic change depends on the GC-content and is affected by specific methylation of guanine residues and by protonation of the GC pairs.The results are compared with literature data, and can be interpreted in terms of a coordination of Cu2+ with GC pairs: a resonable value for the binding of Cu2+ t o DNA at 25" appears to be 1 Cu2f per GC pair.I n the past years several investigators have shown that Cua+ ions may produce a drastic decrease in the helix stability of DNA against thermal denaturation when present at certain Cuz+ concentrations and at low ionic strength [i--8]. These aspects may be of biological interest, since it was shown that carefully isolated DNA samples contained specific amounts of copper and some other metals MATERIALS AND METHODSThe DNA samples used in this work are described elsewhere [17,18]. Nucleosides were purchased from Serva-Entwicklungslabor (Heidelberg), Fluka A. G., and Arcochemie (Berlin). Inorganic chemicals were reagent grade; for low ionic strength solutions sodium salts were used with impurities of divalent meta1 ions below 0.005 Ole.Methylated DNA was prepared according to the method of Pochon and Michelson [i9] with some modification. mi-n-butylamine and dimethyl sulphate were added as a mixture to the precooled DNA solution. The mixture was carefully shaken with ether to separate tri-n-butylamine after reaction;

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Section: Xpectrophotometric Titrationsupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

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Influence of Copper2+ Ions on the Denaturation and Spectral Properties of DNA and Methylated DNA

Zimmer¹,

Venner²

1970

Eur J Biochem

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“…Among the transition metals, copper is an important structural metal in chromatin (23,24) and it can form stable complex with DNA [The association constants are 10 9 M for Cu(I)/DNA and 10 4 M for Cu(II)/DNA] (25)(26)(27). Although debate exists in the literature about the importance of the roles of copper in H 2 O 2 -induced DNA damage in vivo (28,29), most researchers believe that copper plays a significant role in H 2 O 2 -mediated DNA damage (5,23,29,30).…”

Section: Introductionmentioning

confidence: 99%

Identification and Quantification of a Guanine−Thymine Intrastrand Cross-Link Lesion Induced by Cu(II)/H₂O₂/Ascorbate

Hong

Cao

Wang

et al. 2006

Chem. Res. Toxicol.

136

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Reactive oxygen species (ROS) can be induced by both endogenous and exogenous processes and they can damage biological molecules including nucleic acids. It was shown that X-or γ-ray irradiation of aqueous solutions of DNA, during which · OH is one of the major ROS, can lead to the formation of intrastrand crosslink lesions where the neighboring nucleobases in the same DNA strand are covalently bonded. Previous 32 P-postlabeling studies suggested that the intrastrand crosslink lesions may arise from Fenton reaction, which also induces the formation of · OH; the structures of the proposed intrastrand crosslink lesions, however, have not been determined. Here we showed for the first time that the treatment of calf thymus DNA with Cu(II)/H 2 O 2 /ascorbate could lead to the formation of an intrastrand crosslink lesion, i.e., G^T, where the C8 of guanine is covalently bonded to the neighboring 3′ thymine through its methyl carbon. LC-MS/MS quantification results showed dose-responsive formation of G^T. In addition, the yield of the intrastrand crosslink was approximately three orders of magnitude lower than those of commonly observed single-base lesions, that is, 8-oxo-7,8-dihydro-2′-deoxyguanosine, 5-(hydroxymethyl)-2′-deoxyuridine and 5-formyl-2′-deoxyuridine. The induction of intrastrand crosslink lesion in calf thymus DNA by Fenton reagents in vitro suggests that this type of lesion might be formed endogenously in mammalian cells.

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“…Metal-ion binding to nucleic acid has been studied by several techniques including conductimetry [3][4][5] (Mg 2+ -DNA, Ca 2+ -DNA), UV-Vis spectroscopy [5][6][7][8][9][10][11][12][13][14][15][16] (Mg 2+ -DNA, Ca 2+ -DNA, Cu 2+ -DNA, Mn 2+ -DNA, Ni 2+ -DNA, Cd 2+ -DNA, Hg 2+ -DNA, Cr 3+ -DNA, Zn 2+ -DNA), equilibrium dialysis [17][18][19] (Mg 2+ -DNA, Ca 2+ -DNA, Co 2+ -DNA, Zn 2+ -DNA) and X-ray crystallography [20][21][22][23] (Ca 2+ -DNA, Mg 2+ -DNA, Na + -DNA, Li + -DNA). However, all these techniques present considerable experimental difficulties or limitations.…”

Section: Introductionmentioning

confidence: 99%

Zinc binding to lambda phage DNA studied by voltammetric techniques

Souza¹,

Castro²,

Bloch³

2000

J. Braz. Chem. Soc.

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A interação do zinco com o DNA do fago λ foi investigada utilizando a voltametria de pulso diferencial e a voltametria cíclica. Os métodos baseiam-se no monitoramento direto da corrente de redução e oxidação do zinco na ausência e na presença do DNA viral. Curvas de titulação do Zn 2+ com o DNA do fago λ foram obtidas nos intervalos de concentração 3,57 x 10 -12 a 3,92 x 10 -11 mol L -1 e 6,97 x 10 -12 a 5,56 x 10 -11 mol L -1 . Estes dados foram utilizados para o cálculo da constante de dissociação do complexo e da estequiometria. O mecanismo da reação foi estudado utilizando a voltametria cíclica. Curvas do tipo I (corrente de oxidação do zinco) versus v 1/2 (raiz quadrada da velocidade de varredura) mostraram que o processo oxidação-redução do zinco deixa de ser difusional na presença do DNA do fago λ.Binding of zinc to lambda phage DNA was investigated by differential pulse voltammetry and cyclic voltammetry. These methods rely on the direct monitoring of reduction and oxidation current of zinc in the absence and presence of this virion DNA. Titration graphs of Zn 2+ with DNA were obtained in the concentration ranges from 3.57 x 10 -12 to 3.92 x 10 -11 mol L -1 and 6.97 x 10 -12 to 5.56 x 10 -11 mol L -1 . These data were used to calculate the dissociation constant of the complex and the stoichiometry. The mechanism of this reaction was studied by cyclic voltammetry through curves I (oxidation current of Zn 2+ ) versus v 1/2 (square root of scan rate). These curves showed that the oxidation-reduction process of Zn 2+ was not controlled by diffusion in the presence of lambda phage DNA.

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Interaction of Copper(II) with Deoxyribonucleic Acid below 30°^*

Cited by 54 publications

References 14 publications

Influence of Copper2+ Ions on the Denaturation and Spectral Properties of DNA and Methylated DNA

Influence of Copper2+ Ions on the Denaturation and Spectral Properties of DNA and Methylated DNA

Identification and Quantification of a Guanine−Thymine Intrastrand Cross-Link Lesion Induced by Cu(II)/H₂O₂/Ascorbate

Zinc binding to lambda phage DNA studied by voltammetric techniques

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Interaction of Copper(II) with Deoxyribonucleic Acid below 30°*

Cited by 54 publications

References 14 publications

Influence of Copper2+ Ions on the Denaturation and Spectral Properties of DNA and Methylated DNA

Influence of Copper2+ Ions on the Denaturation and Spectral Properties of DNA and Methylated DNA

Identification and Quantification of a Guanine−Thymine Intrastrand Cross-Link Lesion Induced by Cu(II)/H2O2/Ascorbate

Zinc binding to lambda phage DNA studied by voltammetric techniques

Contact Info

Product

Resources

About

Interaction of Copper(II) with Deoxyribonucleic Acid below 30°^*

Identification and Quantification of a Guanine−Thymine Intrastrand Cross-Link Lesion Induced by Cu(II)/H₂O₂/Ascorbate