Interaction of CT-DNA with Ruthenium(II) Metallosurfactant Complexes: Synthesis, CMC Determination, Antitumour and Antimicrobial Activities

Abstract

To enhance the application of metalosurfactants in the field of drug delivery, it is essential to acquire the role of surfactants in terms of micellization, hydrophobicity, interaction with nucleic acids, reactive changes with abnormal cells and pathogenic organisms. A new class of two ruthenium(II) metallosurfactant complexes [Ru(DMP)2(CA)Cl](ClO4) (1) and [Ru(DMP)2(CA)2](ClO4)2 (2), where DMP = 2,9-dimethyl[1,10]phenanthroline) and CA = cetyl amine were synthesized and characterized. The critical micelle concentration (CMC) and the thermodynamic parameters of micellization were determined and the variations suggest the expression of hydrophobic interaction in these complexes. The binding affinity of ruthenium(II) metallosurfactant complexes with CT-DNA has been investigated by spectroscopic and viscosity magnitudes. The outcomes expose that the complexes associate with CT-DNA through intercalation mode. Subsequently the complexes were taken for in vitro anticancer and antimicrobial inhibition study against human cervical cancer cell lines (HeLa) and pathogenic microorganisms and found that the complexes exhibited remarkable inhibitory action. The cytotoxic nature of the complexes towards, HeLa cells, was adopted by MTT assay and apoptosis were examined by AO/EB (acridine orange/ethidium bromide) and tryphanblue staining methods showing that complexes affected the viability of the cells significantly.

Biological properties and conformational studies of amphiphilic Pd(II) and Ni(II) complexes bearing functionalized aroylaminocarbo-N-thioylpyrrolinate units

“…In this line, metal complexes incorporating an amphiphilic character (also called metallosurfactants) exhibit very interesting properties, but their biological and medicinal applications have not been fully developed yet [1,2]. One of the most promising areas of interest in medicine is oncology [3][4][5] and infectious diseases [6][7][8] (or both) [9]. The modulation of the hydrophobicity domain, the influence of the strength of the ligand and the metal cation sphere (together with small molecules coordinated in its outer sphere) are crucial points to study the structure-activity relationship (SAR) onto a fixed biological target [10][11][12].…”

Metallo-Liposomes Derived from the [Ru(bpy)3]2+ Complex as Nanocarriers of Therapeutic Agents

“…The incorporation of metal ions into these nanostructures has improved the transfection processes of several nanocarriers [17] and has also showed a decrease in the energy 2 of 19 status in tumor cells that provokes the deactivation of the cell functions and their subsequent death [18]. Metallo-surfactants of Fe(II), Ni(II), Co(II) and Cu(II) are able to provoke changes in the primary and/or secondary structures of the bovine serum albumin protein, BSA, depending on the type of assembly formed [19].…”