Interaction of .DELTA.- and .LAMBDA.-[Ru(phen)2DPPZ]2+ with DNA: A Calorimetric and Equilibrium Binding Study

Haq, Ihtshamul; Lincoln, Per; Suh, Dongchul; Nordén, Bengt; Chowdhry, Babur Z.; Chaires, Jonathan B.

doi:10.1021/ja00122a008

Cited by 512 publications

(471 citation statements)

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“…It must be pointed out that values of s smaller than unity are not uncommon, and similar values have been found for metal complexes-DNA systems with ascertained DNA intercalating interaction [30,31,35], also possibly showing other interaction mechanisms besides DNA-intercalation. However, the physical interpretation of this parameter is still a matter of debate [35].…”

Section: Uv-vis Absorption Spectroscopysupporting

confidence: 59%

“…For example, Haq et al [35] have found two different values of s for the D and K enantiomers of the [Ru(phen) 2 dppz] 2+ complex interacting with DNA. The first value corresponds to a 1:3 complex-to-DNA base pair molar ratio, while the second one occurs at a drug mole fraction of 0.58 for D-[Ru(phen) 2 dppz] 2+ and of 0.68 for K-[Ru(phen) 2 dppz] 2+ .…”

Section: Uv-vis Absorption Spectroscopymentioning

confidence: 99%

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The interaction of native calf thymus DNA with FeIII-dipyrido[3,2-a:2′,3′-c]phenazine

Terenzi

Barone

Silvestri

et al. 2009

Journal of Inorganic Biochemistry

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a b s t r a c tThe mono and bis dipyrido[3,2-a:2 0 ,3 0 -c]phenazine (dppz) adducts of iron(III) chloride, i.e. [Fe(dppz)]Cl 3 and [Fe(dppz) 2 ]Cl 3 , have been synthesized and characterized. The interaction of the Fe III dppz hydrolyzed aquo complex with native calf thymus DNA has been monitored as a function of the metal complex-DNA molar ratio, by variable temperature UV absorption spectrophotometry, circular dichroism (CD) and fluorescence spectroscopy. The results obtained in solution at various ionic strength values give support for a tight intercalative binding of the Fe III dppz cation with DNA. In particular, the appearance of induced CD bands, caused by the addition of Fe III dppz, indicate the existence of a rigid metal complex-DNA-binding leading to dominating chiral organization of Fe III dppz species within the DNA double helix. The trend of selected CD bands with the molar concentration of Fe III dppz emphasizes that the presence of high amounts of metal complex induces also the formation of DNA-Fe III dppz supramolecular aggregates in solution. The analysis of fluorescence measurements allowed us to calculate a value of the intercalative binding constant comparable to that obtained by UV spectrophotometric titration. Finally, the temperature dependence of the absorbance at 258 nm shows that the metal complex strongly increases the DNA melting temperature already at metal complex-DNA molar ratio equal to 0.25 suggesting that metal complex intercalation effectively hinders DNA denaturation. Overall, the results of the present study point out that the Fe III dppz aquo complex has DNA-binding properties analogous to those previously reported for the tris-chelate Fe II (phen) 2 dppz complex (phen = 1,10-phenantroline).

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Section: Uv-vis Absorption Spectroscopysupporting

confidence: 59%

Section: Uv-vis Absorption Spectroscopymentioning

confidence: 99%

The interaction of native calf thymus DNA with FeIII-dipyrido[3,2-a:2′,3′-c]phenazine

Terenzi

Barone

Silvestri

et al. 2009

Journal of Inorganic Biochemistry

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“…This value is also in good agreement with classical measurements of Ru(bpy) 2 dppz 2+ binding, which was determined to be (6.3 6 0.9) Â 10 5 M À1 . 85 Most important, intercalation was clearly demonstrated for Ru(phen) 3 2+ in DNA stretching experiments that showed an increase in contour length upon its binding. 28 …”

Section: Dna Binding In Optical Tweezers Experimentsmentioning

confidence: 97%

“…The basic structures consist of a Ru(II) surrounded by three heterocyclic, aromatic ligands. [84][85][86] Specific structures include Ru(phen) 3 2+ and Ru(bpy) 2 dppz 2+ . Recent AFM studies have determined the binding constant of Ru(bpy) 2 dppz 2+ to be (1.5 6 0.7) Â 10 5 M À1,28 by examining the length of the DNA as a function of ligand concentration.…”

Section: Ruthenium Complexes Studied With Tweezers and Afmmentioning

confidence: 99%

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Mechanisms of DNA binding determined in optical tweezers experiments

McCauley¹,

Williams²

2006

Biopolymers

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The last decade has seen rapid development in single molecule manipulation of RNA and DNA. Measuring the response force for a particular manipulation has allowed the free energies of various nucleic acid structures and configurations to be determined. Optical tweezers represent a class of single molecule experiments that allows the energies and structural dynamics of DNA to be probed up to and beyond the transition from the double helix to its melted single strands. These experiments are capable of high force resolution over a wide dynamic range. Additionally, these investigations may be compared with results obtained when the nucleic acids are in the presence of proteins or other binding ligands. These ligands may bind into the major or minor groove of the double helix, intercalate between bases or associate with an already melted single strand of DNA. By varying solution conditions and the pulling dynamics, energetic and dynamic information may be deduced about the mechanisms of binding to nucleic acids, providing insight into the function of proteins and the utility of drug treatments. © 2006 Wiley Periodicals, Inc. Biopolymers 85:154–168, 2007.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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Mono‐ and polynuclear ruthenium(II) complexes, photo‐probes and reagents for targeted DNA sites

Mesmaeker

Moucheron

Boutonnet

1998

J. Phys. Org. Chem.

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Interaction of .DELTA.- and .LAMBDA.-[Ru(phen)2DPPZ]2+ with DNA: A Calorimetric and Equilibrium Binding Study

Cited by 512 publications

References 0 publications

The interaction of native calf thymus DNA with FeIII-dipyrido[3,2-a:2′,3′-c]phenazine

The interaction of native calf thymus DNA with FeIII-dipyrido[3,2-a:2′,3′-c]phenazine

Mechanisms of DNA binding determined in optical tweezers experiments

Mono‐ and polynuclear ruthenium(II) complexes, photo‐probes and reagents for targeted DNA sites

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