Interaction of Fluorescein with Felodipine: A Spectrofluorometric and Thermodynamic Study

“…The sensitivity/LOD of our proposed method is detailed in other published methods. The LOD value of our method showed a greater sensitivity with a lower detection limit compared with other methods for LOD was in range 1 × 10– 4 mol•L −1 for detection of FL Na + .…”

Section: Resultsmentioning

confidence: 79%

“…Also it deals with the development of a FRET probe sensor system using PVP‐ANPs as‐prepared by a reprecipitation method and characterized to detect FL Na + in aqueous solutions. The present sensing strategy proved to be more selective and superior than already reported methods for FL Na + detection . Finally, based on the fluorescence quenching results, a fluorimetric method was developed for detection of FL Na + from ophthalmic samples.…”

Section: Introductionmentioning

confidence: 76%

Polyvinyl pyrrolidone capped fluorescent anthracene nanoparticles for sensing fluorescein sodium in aqueous solution and analytical application for ophthalmic samples

et al. 2015

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Based on the known complexation ability between polyvinyl pyrrolidone (PVP) and fluorescein sodium (FL Na(+)), fluorescent PVP capped anthracene nanoparticles (PVP-ANPs) were prepared using a reprecipitation method for detection of fluorescein in aqueous solution using the fluorescence resonance energy transfer (FRET) approach. A dynamic light scattering histogram of PVP-ANPs showed narrower particle size distribution and the average particle size was 15 nm. The aggregation-induced enhanced emission (AIEE) of PVP-ANPs was red shifted from its monomer by 1087.22 cm(-1). The maximum emission was seen to occur at 420 nm. The presence of FL Na(+) in the vicinity of PVP-ANPs quenched the fluorescence of PVP-ANPs because of its adsorption on the surface of PVP-ANPs in aqueous suspension. The FL Na(+) and PVP-ANPs were brought close enough, typically to 7.89 nm, which was less than the distance of 10 nm that is required between the energy donor-acceptor molecule for efficient FRET. The quenching results fit into the Stern-Volmer relationship even at temperatures greater than ambient temperatures. The thermodynamic parameters determined from FRET results helped to propose binding mechanisms involving hydrophobic and electrostatic molecular interaction. The fluorescence quenching results were used further to develop an analytical method for estimation of fluorescein sodium from ophthalmic samples available commercially in the market.

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“…Stern–Volmer quenching constants are positively linked with temperature in dynamic quenching while in the case of static quenching reverse trend is observed. Experimental results indicate increase in K sv values with rise in temperature, manifesting that the quenching mechanism between Trp and NORF was likely to occur via dynamic quenching …”

Section: Resultsmentioning

confidence: 89%

Exploration of Fluorescence Quenching Mechanism in Tryptophan Induced by Norfloxacin: Analytical Applications

Mahanwar

Patil

Naik

et al. 2019

Macromolecular Symposia

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Fluorescence quenching mechanism of tryptophan (Trp) by norfloxacin (NORF) was investigated by spectrofluorometric method at physiological pH. Stern−Volmer quenching constant is positively related with temperature shows the dynamic type of quenching. Life time of Trp decreases by the addition of NORF supports dynamic type of quenching. Fluorescence quenching method was successfully applied for direct determination of NORF from pharmaceutical sample.

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Interaction of Fluorescein with Felodipine: A Spectrofluorometric and Thermodynamic Study

Cited by 17 publications

References 26 publications

Effect of temperature and pH on interaction between bovine serum albumin and cetylpyridinium bromide: Fluorescence spectroscopic approach

Effect of temperature and pH on interaction between bovine serum albumin and cetylpyridinium bromide: Fluorescence spectroscopic approach

Polyvinyl pyrrolidone capped fluorescent anthracene nanoparticles for sensing fluorescein sodium in aqueous solution and analytical application for ophthalmic samples

Exploration of Fluorescence Quenching Mechanism in Tryptophan Induced by Norfloxacin: Analytical Applications

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