Interaction of Gila monster venom with secretin receptors in rat pancreatic membranes

Gillet, Laurent; Robberecht, Patrick; Waelbroeck, Magalì; Camus, Jean Claude; Neef, Philippe De; König, Wolfgang; Christophe, Jean

doi:10.1016/0196-9781(84)90244-4

Cited by 12 publications

(6 citation statements)

References 5 publications

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“…These results confirm our previous finding of an interaction of helodermin with secretin and VIP receptors in rat pancreas [3,181 and VIP receptors in rat hepatic membranes [19].…”

Section: Discussionsupporting

confidence: 82%

“…In 1983 similar observations were made by Amiranoff et al [2] using isolated intestinal glands as a target system, and by ourselves on rat pancreatic plasma membranes [3]. We also…”

supporting

confidence: 59%

“…pancreatic adenylate cyclase activity after occupancy of secretin receptors [3]. In 1984 we purified to homogeneity from the venom a peptide we called helodermin (Hd) [4, 51 whose 35-amino-acid sequence was established by Hoshino et al [6].…”

mentioning

confidence: 99%

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Chemical, immunological and biological properties of peptides like vasoactive‐intestinal‐peptide and peptide‐histidine‐isoleucinamide extracted from the venom of two lizards (Heloderma horridum and Heloderma suspectum)

Vandermeeers¹,

Gourlet²,

Vandermeers‐Piret³

et al. 1987

European Journal of Biochemistry

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1. Having previously isolated helodermin, the major peptide like vasoactive-intestinal-peptide and peptidehistidine-isoleucinamide, from the venom of the lizard Heloderma suspecturn, we decided on a systematic exploration of all (VIP-PHI)-like peptides present in the venom of another lizard of the Helodermatidae family: Heloderma horridurn.2. Six (VIP-PHI)-like peptides (PHHI to 6) were purified to homogeneity from the venom of the lizard H . horridurn with PHH3 and PHH4 representing two minor forms. All peptides cross-reacted in radioimmunoassays for helodermin and PHI but not for VIP. They yielded four fragments (T1 to T4) after trypsin digestion. T1, T2 and T3 showed the same retention time by reverse-phase HPLC and the same amino acid composition; the differences were confined to T4, the C-terminal sequence. PHH5 and PHH6 were found to be identical to synthetic helospectins I and I1 respectively. PHHl and PHH3 probably resulted from a secondary modification of PHHS, while PHH2 and PHH4 derived from PHH6. Thus, the VIP-like peptides, previously called helospectins, are in fact typical of H . horridum venom.3. We confirmed that helodermin is the major (VIP-PHI)-like peptide of the venom of H . suspecturn and observed its absence in H . horridurn venom. Also, we found that positions 8 and 9 of helodermin are occupied by two Glu residues instead of two Gln as previously published. Helospectin-like material was also present in H . suspecturn venom but in very small amount.4. In both venoms all VIP-like peptides were equally potent and efficient when tested for (a) their ability to occupy VIP as well as secretin receptors in rat pancreatic membranes and VIP receptors in rat liver membranes, and (b) the ensuing activation of adenylate cyclase in both membrane preparations.In 1982 Raufman et al. 111 described the presence, in the found that the active material in the venom stimulated rat venom of the lizard Heloderma suspectum (also called Gila monster), of a biologically active material closely related to vasoactive intestinal peptide (VIP), as it inhibited 1251-VIP binding, stimulated cyclic AMP production and promoted amylase secretion in dispersed guinea-pig pancreatic acini. In 1983 similar observations were made by Amiranoff et al. [2] using isolated intestinal glands as a target system, and by ourselves on rat pancreatic plasma membranes [3]. We also

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“…These results confirm our previous finding of an interaction of helodermin with secretin and VIP receptors in rat pancreas [3,181 and VIP receptors in rat hepatic membranes [19].…”

Section: Discussionsupporting

confidence: 82%

“…In 1983 similar observations were made by Amiranoff et al [2] using isolated intestinal glands as a target system, and by ourselves on rat pancreatic plasma membranes [3]. We also…”

supporting

confidence: 59%

See 1 more Smart Citation

Chemical, immunological and biological properties of peptides like vasoactive‐intestinal‐peptide and peptide‐histidine‐isoleucinamide extracted from the venom of two lizards (Heloderma horridum and Heloderma suspectum)

Vandermeeers¹,

Gourlet²,

Vandermeers‐Piret³

et al. 1987

European Journal of Biochemistry

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“…Helodermin displays sequence homologies with peptides of the secretin family (1)(2)(3)(4). Not surprisingly, helodermin exerts secretin-like and vasoactive intestinal peptide (VIP)-like actions in several biological systems (5)(6)(7).…”

mentioning

confidence: 99%

“…45CaC12 (specific radioactivity 4-50 Ci/g) was administered s.c. to fasted rats (see above) at 5 ,uCi/kg (2 x 106 cpm to a 200-g rat. Fifteen minutes later the animals received an s.c. injection with helodermin, VIP, or CT (for doses see Results), and 55 min after the administration of 45Ca they were killed by an overdose of diethyl ether.…”

mentioning

confidence: 99%

Helodermin-like peptides in thyroid C cells: stimulation of thyroid hormone secretion and suppression of calcium incorporation into bone.

Grunditz¹,

Persson²,

Håkanson³

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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Helodermin is a vasoactive intestinal peptidelike peptide in the salivary gland venom of the lizard Heloderma suspectum. Helodermin-like immunofluorescence was observed in the parafollicular (C) cells in several mammals and in the C cell homologues of the chicken ultimobranchial gland. Thus, helodermin-like peptides coexist with calcitonin. The results of radioimmunoassay agreed with the immunocytochemical rindings. HPLC of rat thyroid extracts revealed one major peak of helodermin-like immunoreactivity, which eluted in a position close to that of lizard helodermin. Helodermin stimulated basal thyroid hormone secretion and colloid droplet formation in conscious mice. The effect of large doses of helodermin was quite long-lasting and the maximal response occurred after 2-6 hr. In addition, helodermin suppressed the incorporation of calcium into bone in conscious rats. The findings suggest that helodermin-like peptides in C cells may be involved in the local regulation of thyroid hormone secretion and in the maintenance of calcium homeostasis.Helodermin is an amidated 35-amino acid peptide isolated from salivary gland venom of the Gila monster lizard (Heloderma suspectum) (1, 2). Helodermin displays sequence homologies with peptides of the secretin family (1-4). Not surprisingly, helodermin exerts secretin-like and vasoactive intestinal peptide (VIP)-like actions in several biological systems (5-7).Immunochemical analyses have revealed small amounts of helodermin-like material in extracts of brain, salivary glands, and intestines of mammals (8,9). Recently, we showed helodermin-like immunoreactivity in cells in thyroid medullary carcinomas and parafollicular (C)-cell adenomas, where the helodermin-like material was found to coexist with calcitonin (CT) (10). The occurrence of helodermin-like immunoreactivity in normal human C cells suggested that helodermin-like peptides are normal constituents of such cells (10).This study confirms the presence of helodermin-like peptides in thyroid C cells of several mammals. Immunoreactive helodermin was determined by RIA and analyzed by HPLC. The effect of synthetic helodermin on thyroid hormone secretion was tested in mice, while its effect on calcium incorporation in bone was studied in rats. MATERIALS AND METHODSTissue Material. Specimens from thyroid glands (at least ten from each species) were collected for immunocytochemistry and RIA from chickens, mice (NMRI), rats (SpragueDawley), hamsters, guinea pigs, rabbits, pigs, cows, and humans, and ultimobranchial glands (UBG) were collected from chickens. In addition, specimens from salivary glands, heart, lungs, gastrointestinal tract (esophagus, stomach, duodenum, jejunum, and colon), liver, pancreas, spleen, and kidneys of rats (200-g body weight) and pigs were collected.Immunocytochemistry. Helodermin antiserum. Antisera were raised in rabbits against unconjugated synthetic helodermin (Peninsula Laboratories). The peptide, 0.5 mg in 1 ml of 0.9% NaCl, was emulsified with 1.0 ml of Freund's complete adjuvant (Difco)...

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Immunoreactive helodermin-like peptides in rat: A new class of mammalian neuropeptides related to secretin and VIP

Robberecht¹,

Graef²,

Woussen³

et al. 1985

Biochemical and Biophysical Research Communications

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Helodermin is a peptide from the venom of the lizard Heloderma suspectum (Gila Monster) showing a high degree of sequence similarity with VIP, PHI and secretin in its N-terminal moiety. The present data support the presence of peptide(s) closely related to helodermin in the brain, gut and salivary glands of rat. In our radioimmunoassays, we routinely used one of the three specific antisera obtained from rabbits that were immunized against lizard helodermin coupled to bovine serum albumin with carbodiimide. Heat- and acid-stable immunoreactive helodermin-like material was more abundant in striatum, hippocampus and anterior pituitary than in cerebral cortex and hypothalamus. High levels of helodermin-like material were also present in salivary glands, duodenum and jejunum. When submitted to gel permeation chromatography on a TSK-G 2000 SW column, the apparent molecular radius of most of the immunoreactive material ranged from 6 to 12 KDa.

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Interaction of Gila monster venom with secretin receptors in rat pancreatic membranes

Cited by 12 publications

References 5 publications

Chemical, immunological and biological properties of peptides like vasoactive‐intestinal‐peptide and peptide‐histidine‐isoleucinamide extracted from the venom of two lizards (Heloderma horridum and Heloderma suspectum)

Chemical, immunological and biological properties of peptides like vasoactive‐intestinal‐peptide and peptide‐histidine‐isoleucinamide extracted from the venom of two lizards (Heloderma horridum and Heloderma suspectum)

Helodermin-like peptides in thyroid C cells: stimulation of thyroid hormone secretion and suppression of calcium incorporation into bone.

Immunoreactive helodermin-like peptides in rat: A new class of mammalian neuropeptides related to secretin and VIP

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