“…However, as a careful inspection of even raw spectra can unravel certain molecular-level details, the analysis of baseline-corrected and smoothed spectral data was performed. Therefore, obtained spectra were examined in the following spectral ranges: (i) 2890–2820 cm −1 , in which both antisymmetric (ν as CH 2 ) and symmetric (ν s CH 2 ) stretching of methylene groups of DPPC hydrocarbon chains appear, (ii) 1800–1480 cm −1 , in which the band originating from carbonyl stretching of lipid glycerol backbone (νC=O(OR)) appears, along with Amide I ((νC=O(NH)),) and Amide II (δNH)) bands of MBP [ 35 ], (iii) 1490–1430 cm −1 , which contains the signatures of DPPC methylene groups scissoring (γCH 2 ) and the bending of protonated amino moiety (δNH 3 + ) of MBP [ 59 ], (iv) 1335–1275 cm −1 comprising Amide III signature dominated by the bending of secondary amine (δN–H) coupled with stretching of CN (νC–N) and bending of CH (δCH) moiety of MBP [ 60 , 61 ], (v) 1275–1190 cm −1 , which reveals the signatures of phosphate moieties of lipid polar headgroups (ν as PO 2 − ) and bending of secondary amine (δN–H) coupled with stretching of CN moiety (νC–N) and bending of CH moiety (δCH) [ 60 ] of MBP, and (vi) 1015–935 cm −1 containing the signature of a choline group of lipid molecules (ν (a)s C–N(CH 3 ) 3 + ) [ 62 , 63 ]. In the selected ranges, the spectra were smoothed (Savitzky–Golay procedure using the polynomial of a 3rd degree through 10/40 points (256/128 scans) and baseline-corrected (2 points at spectral minima) [ 54 ].…”